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[177334_at]

Description M176.1 /REP_DB=WormBase Gene ID /WP=CE12460 /TR=Q21550 /GB=CAB01649.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 43      4grp_total > 34      6grp_cgc > 91      6grp_cgc_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 3.3
(-3)
A 		45.4
(3)
P 		5.2
(-2.7)
A 		27.6
(0.5)
A 		42
B 16.3
(-1.6)
A 		48
(3)
A 		42
(2.1)
A 		6.7
(-3)
A 		41
C 25.3
(-3)
A 		29.6
(-2.4)
A 		31
(-2.2)
A 		66.5
(3)
A 		41
avr 15
(-3) 		41
(3) 		26.1
(-0.4) 		33.6
(1.3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 17.9
(-0.9)
A 		11.4
(-1.9)
A 		44.1
(3)
A 		40.2
(2.4)
A 		3.9
(-3)
A 		10
(-2.1)
A 		40
B 26.2
(0.3)
A 		8.4
(-3)
A 		40.3
(3)
A 		23.5
(-0.2)
A 		21.1
(-0.6)
A 		8.6
(-3)
A 		32
C 46.9
(3)
A 		3.8
(-3)
A 		42.3
(2.4)
A 		6.5
(-2.6)
A 		20.3
(-0.7)
A 		23.4
(-0.3)
A 		43
avr 30.3
(0.9) 		7.9
(-3) 		42.2
(3) 		23.4
(-0.3) 		15.1
(-1.7) 		14
(-1.9) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1.2
(-3)
A 		9.9
(3)
P 		1.4
(-2.9)
A 		4
(-1.1)
A 		9
B 5.2
(-0.8)
A 		11.9
(3)
A 		9.7
(1.7)
A 		1.4
(-3)
A 		11
C 8.4
(-3)
A 		8.5
(-2.8)
A 		10.8
(2.1)
A 		11.2
(3)
A 		3
avr 4.9
(-3) 		10.1
(3) 		7.3
(-0.2) 		5.5
(-2.3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 3.3
(-1.6)
A 		4.1
(-1.1)
A 		9.6
(2.6)
A 		10.2
(3)
A 		1.2
(-3)
A 		2.4
(-2.2)
A 		9
B 9.1
(2.4)
A 		2.8
(-3)
A 		9.8
(3)
A 		6.2
(-0.1)
A 		8.6
(2)
A 		3.2
(-2.7)
A 		7
C 17.4
(3)
A 		1.2
(-3)
A 		10.8
(0.6)
A 		1.3
(-3)
A 		6.6
(-1)
A 		7.8
(-0.6)
A 		16
avr 9.9
(2.8) 		2.7
(-3) 		10.1
(3) 		5.9
(-0.4) 		5.5
(-0.7) 		4.5
(-1.5) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177334_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3780
Sequence Type Exemplar sequence
Representative Public ID M176.1 NCBI
Target Description M176.1 /REP_DB=WormBase Gene ID /WP=CE12460 /TR=Q21550 /GB=CAB01649.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063609(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063609 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-50) (tag-50) mRNA, complete cds. 11/11 None
SNAP00000024999 ENSEMBL cdna:SNAP chromosome:CEL140:II:9412477:9414056:1 11/11 None
M176.1 ENSEMBL cdna:known chromosome:CEL140:II:9412477:9414056:1 gene:M176.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000025016 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9412473-9414053(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-50
Ensembl M176.1
Entrez Gene 187498 Entrez gene
SwissProt Q21550 EMBL-EBI
WormBase CE12460 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01649 Hypothetical protein M176.1 [Caenorhabditis elegans] ref|NP_496010.1| Temporarily Assigned Gene name family member (tag-50) [Caenorhabditis elegans] 1.0E-161
blast CAE57953 Hypothetical protein CBG01012 [Caenorhabditis briggsae] 8.0E-27
blast CAA19457 Hypothetical protein Y17G7B.11 [Caenorhabditis elegans] ref|NP_496567.2| Y17G7B.11 [Caenorhabditis elegans] 7.0E-23
Protein Domains
Database ID Description E-Value
Pfam IPR000698 EMBL-EBI Arrestin 1.0E-8
Pfam IPR000698 EMBL-EBI Arrestin 4.7E-13
Sequence
Target Sequence 
>C. ELEGANS:177334_AT
gaatttcacttctgacacggattgcataaaacccggaacacacaaattcccattttcttt
caaattaccagcagattgtccgccaattgtaccgaatgcaacttatataccaaaagactt
gcaacaaatcaatggagctgtttcgaagaatatcggtgtatttttcaagagtgggataac
tagcttcccacagcgggttttaatcacgggagaagttattccactaactttgctgatcga
caataaatcaacatgcacagttcgagaagtcggagtccggattttcagaattgcaagatt
ttatgctaaagaccaggagaaaatgactcggcagaggaaaataatgatacgaaagagcat
caacgtggaaccaaacaccgaacaacaagaactcatcgaatttaaagttcacgaaaccgt
acaaacttttgaaagtgatcttatcgaagtgaagtatttaatgcatgttgatgtatttac
tacatctgcattcagaggcactttaaattcagttttctcagtaattattggagcttcgcc
cacaatgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTTCACTTCTGACACGGATTGC 658 325 285 Antisense
GGATTGCATAAAACCCGGAACACAC 26 509 303 Antisense
ATTACCAGCAGATTGTCCGCCAATT 679 11 348 Antisense
GTCCGCCAATTGTACCGAATGCAAC 50 477 362 Antisense
ATAACTAGCTTCCCACAGCGGGTTT 492 23 460 Antisense
CCACAGCGGGTTTTAATCACGGGAG 421 271 472 Antisense
ATTCCACTAACTTTGCTGATCGACA 516 7 502 Antisense
AGTCGGAGTCCGGATTTTCAGAATT 415 59 552 Antisense
GTATTTACTACATCTGCATTCAGAG 533 453 757 Antisense
GAGGCACTTTAAATTCAGTTTTCTC 132 405 779 Antisense
TTATTGGAGCTTCGCCCACAATGAC 200 679 809 Antisense
Sequence Source Affymetrix Proprietary Database