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[177332_at]

Description CD4.3 /REP_DB=WormBase Gene ID /WP=CE16951 /TR=O44159 /GB=AAB88341.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 37      4grp_highest_8_8 > 32      4grp_high_8_8 > 43      6grp_highest_nonorm > 76      6grp_total > 17      4grp_total > 52      6grp_highest_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 488
1 2 3 4 range
A 1373.5
(3)
P 		1265.7
(1)
P 		1290.8
(1.5)
P 		1048.3
(-3)
P 		325
B 1399.5
(3)
P 		1389.9
(2.7)
P 		1387
(2.7)
P 		1184.6
(-3)
P 		215
C 1461.4
(2)
P 		1536.1
(3)
P 		1249.3
(-0.8)
P 		1084.1
(-3)
P 		452
avr 1411.5
(3) 		1397.2
(2.7) 		1309
(1) 		1105.7
(-3) 		306

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 461
1 2 3 4 5 6 range
A 1360.9
(3)
P 		1175.3
(0)
P 		1071.6
(-1.6)
P 		1018.4
(-2.5)
P 		984.4
(-3)
P 		1242.9
(1.1)
P 		377
B 1407.7
(3)
P 		1149
(-1)
P 		1399.3
(2.9)
P 		1020.2
(-3)
P 		1018.1
(-3)
P 		1166.5
(-0.7)
P 		390
C 1275
(3)
P 		1128.6
(0.3)
P 		1104.1
(-0.1)
P 		1161.2
(0.9)
P 		947
(-3)
P 		1256.7
(2.7)
P 		328
avr 1347.9
(3) 		1151
(-0.2) 		1191.7
(0.4) 		1066.6
(-1.6) 		983.2
(-3) 		1222
(0.9) 		365
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 347
1 2 3 4 range
A 500.1
(3)
P 		274.8
(-0.9)
P 		337.4
(0.2)
P 		153.4
(-3)
P 		347
B 446.3
(3)
P 		343.3
(-0.2)
P 		321.4
(-0.9)
P 		252.4
(-3)
P 		194
C 483.8
(3)
P 		441.6
(2.2)
P 		435.4
(2)
P 		182.8
(-3)
P 		301
avr 476.7
(3) 		353.2
(0.4) 		364.7
(0.6) 		196.2
(-3) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 256
1 2 3 4 5 6 range
A 250.9
(-2.4)
P 		418
(3)
P 		233.4
(-3)
P 		258.3
(-2.2)
P 		297
(-0.9)
P 		302.6
(-0.8)
P 		185
B 489.8
(3)
P 		382.4
(0.1)
P 		339.8
(-1.1)
P 		270.2
(-3)
P 		413.7
(0.9)
P 		435
(1.5)
P 		220
C 472.6
(3)
P 		344.4
(-0.2)
P 		281
(-1.8)
P 		233.5
(-3)
P 		306.4
(-1.2)
P 		420.7
(1.7)
P 		239
avr 404.4
(3) 		381.6
(2.1) 		284.7
(-1.8) 		254
(-3) 		339
(0.4) 		386.1
(2.3) 		150
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177332_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12369
Sequence Type Exemplar sequence
Representative Public ID CD4.3 NCBI
Target Description CD4.3 /REP_DB=WormBase Gene ID /WP=CE16951 /TR=O44159 /GB=AAB88341.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072074(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072074 NCBI Caenorhabditis elegans CD4.3 (CD4.3) mRNA, complete cds. 11/11 None
SNAP00000006232 ENSEMBL cdna:SNAP chromosome:CEL140:V:5596305:5597018:1 11/11 None
GENEFINDER00000006241 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5596305:5597054:1 11/11 None
CD4.3.2 ENSEMBL cdna:known chromosome:CEL140:V:5596301:5597069:1 gene:CD4.3 11/11 None
CD4.3.1 ENSEMBL cdna:known chromosome:CEL140:V:5596304:5597065:1 gene:CD4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5596304-5597018(+) 99.47 100.0
Public Domain and Genome References
Gene Title
Ensembl CD4.3
Entrez Gene 259341 Entrez gene
SwissProt O44159 EMBL-EBI
WormBase CE16951 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1638783_AT mitochondrial ribosomal protein L48 dm
DROSGENOME1:145595_AT mitochondrial ribosomal protein L48 dm
Protein Similarities
Method ID Description E-Value
blast NP_504475 CD4.3 [Caenorhabditis elegans] gb|AAB88341.1| Hypothetical protein CD4.3 [Caenorhabditis elegans] 3.0E-93
blast NP_504475 CD4.3 [Caenorhabditis elegans] gb|AAB88341.1| Hypothetical protein CD4.3 [Caenorhabditis elegans] 4.0E-93
blast CAE64607 Hypothetical protein CBG09363 [Caenorhabditis briggsae] 1.0E-83
blast CAE64607 Hypothetical protein CBG09363 [Caenorhabditis briggsae] 2.0E-83
Sequence
Target Sequence 
>C. ELEGANS:177332_AT
tccttattatctcgcgtgatctcacagaatcgaggactcgcatcgctacgagaaactgtt
ggagagcgtgacgccagtttgctctacgagccgaaatttcaagatagtagagagtttcca
gagtacaacacaattaacgtccgaattcaaggacacgactttggatcgctagaaaaatat
caagcatacattcataaaactgcgaaacgattcggatttaccgtggtggatagttatgct
gtcgctgctcaaacacaaaaagcaatcacctataagccgtactcgacggtttccgaatct
gaaattgatatgtccacatacgatcgcgtcgtccgtctcagtgatgttgccgctccccga
ttctcacttttcagtcaaattattcgagcacatattcccattggagttactatgacggtg
aaagaacacgagaaagttgatgaagactcccgatatattcctgattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTTATTATCTCGCGTGATCTCAC 168 593 31 Antisense
GAATCGAGGACTCGCATCGCTACGA 581 329 57 Antisense
AGAGCGTGACGCCAGTTTGCTCTAC 36 71 93 Antisense
AAGGACACGACTTTGGATCGCTAGA 688 159 179 Antisense
GATAGTTATGCTGTCGCTGCTCAAA 468 425 259 Antisense
CAATCACCTATAAGCCGTACTCGAC 573 181 293 Antisense
CGTACTCGACGGTTTCCGAATCTGA 171 247 308 Antisense
ATATGTCCACATACGATCGCGTCGT 577 21 338 Antisense
CCCGATTCTCACTTTTCAGTCAAAT 237 261 386 Antisense
ATTCGAGCACATATTCCCATTGGAG 392 9 412 Antisense
AAGACTCCCGATATATTCCTGATTT 535 153 473 Antisense
Sequence Source Affymetrix Proprietary Database