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[177323_at]

Description ZC8.1 /REP_DB=WormBase Gene ID /WP=CE07577 /TR=Q23086 /GB=AAB52622.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 13      4grp_total > 30      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 90.6
(3)
P 		12.5
(-3)
A 		38
(-1)
A 		84.7
(2.5)
A 		78
B 149.2
(3)
P 		47.9
(-1.7)
A 		19
(-3)
A 		37.3
(-2.2)
A 		130
C 94.8
(1)
P 		58.7
(-0.6)
A 		4.8
(-3)
A 		140.9
(3)
P 		136
avr 111.5
(3) 		39.7
(-1.7) 		20.6
(-3) 		87.6
(1.4) 		91

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 119
1 2 3 4 5 6 range
A 81.4
(3)
A 		75.8
(2.6)
A 		58.7
(1.2)
A 		38.5
(-0.4)
A 		50.6
(0.6)
A 		4.9
(-3)
A 		77
B 124.3
(3)
P 		93.7
(1.4)
A 		47.8
(-1)
A 		72
(0.3)
A 		37.9
(-1.5)
A 		9.4
(-3)
A 		115
C 41.8
(-1.5)
P 		98.4
(3)
P 		87.3
(2.1)
A 		81.9
(1.7)
A 		44.8
(-1.3)
A 		23.2
(-3)
A 		75
avr 82.5
(2.5) 		89.3
(3) 		64.6
(1.1) 		64.1
(1) 		44.4
(-0.5) 		12.5
(-3) 		77
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 33
(3)
P 		2.7
(-3)
A 		9.9
(-1.6)
A 		12.4
(-1.1)
A 		30
B 47.6
(3)
P 		11.8
(-2)
A 		4.4
(-3)
A 		8
(-2.5)
A 		43
C 31.4
(3)
P 		16.9
(0.1)
A 		1.7
(-3)
A 		23.8
(1.5)
P 		30
avr 37.3
(3) 		10.5
(-2) 		5.3
(-3) 		14.7
(-1.2) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 15
(0.2)
A 		27
(3)
A 		12.8
(-0.3)
A 		9.8
(-1)
A 		15.3
(0.3)
A 		1.2
(-3)
A 		26
B 43.3
(3)
P 		31.2
(1.2)
A 		11.6
(-1.8)
A 		19.1
(-0.6)
A 		15.4
(-1.2)
A 		3.5
(-3)
A 		40
C 15.5
(-0.9)
P 		30
(3)
P 		22.2
(0.9)
A 		16.5
(-0.6)
A 		14.5
(-1.2)
A 		7.8
(-3)
A 		22
avr 24.6
(1.9) 		29.4
(3) 		15.5
(-0.3) 		15.1
(-0.4) 		15.1
(-0.4) 		4.2
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177323_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17181
Sequence Type Exemplar sequence
Representative Public ID ZC8.1 NCBI
Target Description ZC8.1 /REP_DB=WormBase Gene ID /WP=CE07577 /TR=Q23086 /GB=AAB52622.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076450(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076450 NCBI Caenorhabditis elegans ZC8.1 (ZC8.1) mRNA, complete cds. 11/11 None
SNAP00000023546 ENSEMBL cdna:SNAP chromosome:CEL140:X:5005455:5006962:1 11/11 None
GENEFINDER00000023567 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5005455:5006962:1 11/11 None
ZC8.1 ENSEMBL cdna:known chromosome:CEL140:X:5005251:5006962:1 gene:ZC8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5005251-5006963(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZC8.1
Entrez Gene 191049 Entrez gene
SwissProt Q23086 EMBL-EBI
WormBase CE07577 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6694 steroid biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3854 3-beta-hydroxy-delta5-steroid dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52622 Hypothetical protein ZC8.1 [Caenorhabditis elegans] ref|NP_508851.1| ZC8.1 [Caenorhabditis elegans] 0.0
blast AAB52622 Hypothetical protein ZC8.1 [Caenorhabditis elegans] ref|NP_508851.1| ZC8.1 [Caenorhabditis elegans] 1.0E-174
blast CAE68677 Hypothetical protein CBG14581 [Caenorhabditis briggsae] 1.0E-110
blast CAE68677 Hypothetical protein CBG14581 [Caenorhabditis briggsae] 2.0E-90
blast AAL11106 Hypothetical protein ZC449.6 [Caenorhabditis elegans] ref|NP_508852.1| ZC449.6 [Caenorhabditis elegans] 1.0E-77
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508851.1 1 354-373
ZC8.1 1 354-373
SNAP00000023546 1 300-319
GENEFINDER00000023567 1 300-319
Sequence
Target Sequence 
>C. ELEGANS:177323_AT
gaagagagtctgtatcgtgtgcgagtagtggagtcaactgtgcaaaagcattcgccctcg
ctgatcagatgcttcaaaatccggacggattgcatggaagggcctactacattttggacg
gagaaaacgtgggacaattccaattttggacaccattagtccttgcgctgggaaaacaac
caccatcgttctatactccgtacgactttataaaagccgttgtaccatattttcaaaacg
tgtgctacggagtattcaaattaccgccacttctcacaaagtttgagctctccattttag
cagttgataacacgtatagcattgagagggctcgtcgcgagttaggatatgaaccggaac
cctgtgtcatgacagatgttgcaaaatactatcaagatctggaagagaatgaagtagcta
cagcgactacagaatggaaatctgcaatttccatcctcatcgtattcatttcaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAGAGTCTGTATCGTGTGCGAG 438 339 632 Antisense
CAACTGTGCAAAAGCATTCGCCCTC 419 187 666 Antisense
GACGGATTGCATGGAAGGGCCTACT 78 377 715 Antisense
GGGCCTACTACATTTTGGACGGAGA 322 455 731 Antisense
CACCATTAGTCCTTGCGCTGGGAAA 210 201 782 Antisense
AAACAACCACCATCGTTCTATACTC 179 127 805 Antisense
GAGTATTCAAATTACCGCCACTTCT 484 397 881 Antisense
GTTTGAGCTCTCCATTTTAGCAGTT 319 451 912 Antisense
AGCATTGAGAGGGCTCGTCGCGAGT 70 79 949 Antisense
ATGAACCGGAACCCTGTGTCATGAC 29 45 980 Antisense
CCATCCTCATCGTATTCATTTCAAT 446 271 1082 Antisense
Sequence Source Affymetrix Proprietary Database