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[177320_s_at]

Description Y54E10BR.H /REP_DB=WormBase Gene ID /WP=CE22470 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59555.1
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 97      4grp_highest_8_8 > 22      4grp_high_8_8 > 07      6grp_highest_nonorm > 73      6grp_total > 97      4grp_total > 01      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 285
1 2 3 4 range
A 420.3
(-0.6)
P 		367.7
(-3)
P 		410.4
(-1.1)
P 		500
(3)
P 		132
B 387.8
(-2.8)
P 		379.5
(-3)
P 		439.8
(-1.7)
P 		653
(3)
P 		274
C 468.4
(-1.2)
P 		387.7
(-3)
P 		440.1
(-1.8)
P 		650
(3)
P 		262
avr 425.5
(-1.7) 		378.3
(-3) 		430.1
(-1.6) 		601
(3) 		223

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 327
1 2 3 4 5 6 range
A 441.1
(-2.7)
P 		463.1
(-2.3)
P 		427.1
(-3)
P 		552.9
(-0.5)
P 		578.8
(0.1)
P 		724.6
(3)
P 		298
B 463.6
(-2.6)
P 		440.4
(-3)
P 		474
(-2.4)
P 		456.9
(-2.7)
P 		584.6
(-0.2)
P 		754.5
(3)
P 		314
C 512.8
(-1.7)
P 		449.7
(-3)
P 		533.4
(-1.2)
P 		579
(-0.3)
P 		691.3
(2.1)
P 		734.3
(3)
P 		285
avr 472.5
(-2.6) 		451.1
(-3) 		478.2
(-2.4) 		529.6
(-1.4) 		618.2
(0.5) 		737.8
(3) 		287
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 153
(3)
P 		79.8
(-2.5)
P 		107.3
(-0.4)
P 		73.2
(-3)
P 		80
B 123.7
(1)
P 		93.7
(-3)
P 		101.9
(-1.9)
P 		139.2
(3)
P 		46
C 155.1
(3)
P 		111.5
(-2.7)
P 		153.4
(2.8)
P 		109.6
(-3)
P 		46
avr 143.9
(3) 		95
(-3) 		120.9
(0.2) 		107.3
(-1.5) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 81.3
(-3)
P 		164.7
(2.3)
P 		93
(-2.3)
P 		140.2
(0.7)
P 		174.7
(2.9)
P 		176.4
(3)
P 		95
B 161.3
(-1.3)
P 		146.6
(-1.9)
P 		115.1
(-3)
P 		121
(-2.8)
P 		237.5
(1.4)
P 		281.3
(3)
P 		166
C 190.1
(0.4)
P 		137.2
(-2)
P 		135.8
(-2.1)
P 		116.4
(-3)
P 		223.7
(2)
P 		245.8
(3)
P 		129
avr 144.2
(-1.5) 		149.5
(-1.3) 		114.6
(-3) 		125.9
(-2.4) 		212
(1.9) 		234.5
(3) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177320_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18732
Sequence Type Exemplar sequence
Representative Public ID Y54E10BR.H NCBI
Target Description Y54E10BR.H /REP_DB=WormBase Gene ID /WP=CE22470 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59555.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058690(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058690 NCBI Caenorhabditis elegans Y54E10BR.3 (Y54E10BR.3) mRNA, complete cds. 11/11 None
SNAP00000038969 ENSEMBL cdna:SNAP chromosome:CEL140:I:3021665:3031129:1 11/11 None
GENEFINDER00000038979 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3023146:3028164:1 11/11 None
Y54E10BR.3 ENSEMBL cdna:known chromosome:CEL140:I:3021650:3028385:1 gene:Y54E10BR.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3021664-3028164(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E10BR.3
Entrez Gene 171875 Entrez gene
SwissProt Q9N3D1 EMBL-EBI
WormBase CE22470 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59555 Hypothetical protein Y54E10BR.3 [Caenorhabditis elegans] ref|NP_491091.1| Y54E10BR.3 [Caenorhabditis elegans] 1.0E-123
blast AAF59555 Hypothetical protein Y54E10BR.3 [Caenorhabditis elegans] ref|NP_491091.1| Y54E10BR.3 [Caenorhabditis elegans] 1.0E-117
blast AAF59555 Hypothetical protein Y54E10BR.3 [Caenorhabditis elegans] ref|NP_491091.1| Y54E10BR.3 [Caenorhabditis elegans] 2.0E-97
blast CAE60564 Hypothetical protein CBG04193 [Caenorhabditis briggsae] 4.0E-70
blast CAE60564 Hypothetical protein CBG04193 [Caenorhabditis briggsae] 7.0E-70
blast CAE60564 Hypothetical protein CBG04193 [Caenorhabditis briggsae] 5.0E-49
blast XP_850302 PREDICTED: similar to Zinc finger protein 364 (Rabring 7) [Canis familiaris] 1.0E-12
blast XP_623158 PREDICTED: similar to Ring finger protein 126 [Apis mellifera] 1.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR001841 EMBL-EBI Zn-finger, RING 2.0E-11
Pfam IPR001841 EMBL-EBI Zn-finger, RING 2.0E-11
Sequence
Target Sequence 
>C. ELEGANS:177320_S_AT
gcagcagcaaccacatccaaatgtgacattctcattccaaatgcccggtggaattggtgt
tcagattcatgcacatactgccggaggtggtggtggtaacaatccagatggaatgccatt
tgggatggaaattggggaacgtgctgatttggagtctgcaatgcaggagattctggctca
atttcaggctgcgggtggtgaaggtggacaacgtcaaggtggattcacagaagctgaagt
gaaagagtatcttccaatgaaaaaagtgacacaggagcatattgataatggagcacagtg
tactacgtgttttgatacattcaagttggatgaggatgttggtgcactcgactgtaatca
cattttccatcgtccgtgcattgagccgtggcttaagactaagaattcgtgtccggtgtg
ccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGCAGCAACCACATCCAAATGTG 430 313 426 Antisense
ATTCTCATTCCAAATGCCCGGTGGA 231 11 453 Antisense
GATTCATGCACATACTGCCGGAGGT 401 429 489 Antisense
GGGAACGTGCTGATTTGGAGTCTGC 465 493 560 Antisense
GATTCTGGCTCAATTTCAGGCTGCG 402 431 594 Antisense
CAGGCTGCGGGTGGTGAAGGTGGAC 544 207 610 Antisense
GGACAACGTCAAGGTGGATTCACAG 681 525 631 Antisense
GGAGCACAGTGTACTACGTGTTTTG 39 521 715 Antisense
ATGTTGGTGCACTCGACTGTAATCA 58 47 761 Antisense
ACTGTAATCACATTTTCCATCGTCC 587 97 776 Antisense
TAAGAATTCGTGTCCGGTGTGCCGT 699 637 825 Antisense
Sequence Source Affymetrix Proprietary Database