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[177311_at]

Description CD4.8 /REP_DB=WormBase Gene ID /WP=CE16956 /TR=O61192 /GB=AAB88346.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 85      4grp_total > 27      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 47.3
(-1)
A 		65
(0.4)
A 		21.7
(-3)
A 		99
(3)
A 		77
B 61.7
(1.4)
A 		66.3
(3)
A 		49.3
(-3)
A 		61.5
(1.3)
P 		17
C 50.3
(-3)
A 		67.9
(2)
A 		65.4
(1.3)
A 		71.4
(3)
P 		21
avr 53.1
(-1.6) 		66.4
(0.9) 		45.5
(-3) 		77.3
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 6.3
(-3)
A 		36.4
(-1.4)
A 		58.8
(-0.2)
P 		87.9
(1.3)
A 		89.5
(1.4)
A 		120
(3)
P 		114
B 26.7
(-3)
A 		35.6
(-2.2)
A 		57.3
(-0.3)
A 		46.3
(-1.3)
A 		83.5
(1.9)
A 		95.8
(3)
M 		69
C 40.3
(-3)
A 		51.1
(-2.1)
A 		47.1
(-2.4)
A 		113.1
(3)
A 		81.3
(0.4)
P 		93
(1.3)
P 		73
avr 24.4
(-3) 		41
(-1.7) 		54.4
(-0.7) 		82.4
(1.4) 		84.8
(1.6) 		102.9
(3) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 17.2
(3)
A 		14.1
(1.4)
A 		5.7
(-3)
A 		14.5
(1.6)
A 		12
B 19.7
(3)
A 		16.4
(0.6)
A 		11.4
(-3)
A 		13.1
(-1.8)
P 		8
C 16.6
(-0.4)
A 		19.5
(1.2)
A 		22.8
(3)
A 		12
(-3)
P 		11
avr 17.8
(3) 		16.7
(1.6) 		13.3
(-2.9) 		13.2
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 1.2
(-3)
A 		13
(-0.5)
A 		12.8
(-0.5)
P 		22.3
(1.5)
A 		27
(2.5)
A 		29.2
(3)
P 		28
B 9.3
(-3)
A 		11.8
(-2.4)
A 		13.9
(-2)
A 		12.3
(-2.3)
A 		33.9
(2.6)
A 		35.7
(3)
M 		26
C 14.9
(-2.1)
A 		15.6
(-1.9)
A 		12
(-3)
A 		22.7
(0.4)
A 		26.3
(1.5)
P 		31.1
(3)
P 		19
avr 8.5
(-3) 		13.5
(-1.7) 		12.9
(-1.9) 		19.1
(-0.3) 		29.1
(2.3) 		32
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177311_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11468
Sequence Type Exemplar sequence
Representative Public ID CD4.8 NCBI
Target Description CD4.8 /REP_DB=WormBase Gene ID /WP=CE16956 /TR=O61192 /GB=AAB88346.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072077(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072077 NCBI Caenorhabditis elegans CD4.8 (CD4.8) mRNA, complete cds. 11/11 None
GENEFINDER00000006239 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5599721:5603924:-1 10/11 None
CD4.8 ENSEMBL cdna:known chromosome:CEL140:V:5601299:5604066:-1 gene:CD4.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000006236 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1402050-1406069(+) 50.65 50.65
chrV:5601298-5604066(-) 98.65 100.0
chrX:1398991-1400602(-) 62.16 62.16
Public Domain and Genome References
Gene Title
Ensembl CD4.8
Entrez Gene 178947 Entrez gene
SwissProt O61192 EMBL-EBI
WormBase CE16956 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504478 CD4.8 [Caenorhabditis elegans] gb|AAB88346.1| Hypothetical protein CD4.8 [Caenorhabditis elegans] 0.0
blast AAA81756 Hypothetical protein ZK402.5 [Caenorhabditis elegans] ref|NP_508261.1| ZK402.5 [Caenorhabditis elegans] 0.0
blast CAB07289 Hypothetical protein T28A8.6 [Caenorhabditis elegans] ref|NP_499792.1| T28A8.6 [Caenorhabditis elegans] 1.0E-120
blast CAB07289 Hypothetical protein T28A8.6 [Caenorhabditis elegans] ref|NP_499792.1| T28A8.6 [Caenorhabditis elegans] 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR006570 EMBL-EBI SPK 2.6E-57
Pfam IPR006570 EMBL-EBI SPK 1.1E-66
Pfam IPR006570 EMBL-EBI SPK 2.6E-57
Pfam IPR006570 EMBL-EBI SPK 1.1E-66
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000006239 1 588-610
Sequence
Target Sequence 
>C. ELEGANS:177311_AT
ataagattatcctatttcccgagcttcgtggagttttcgaggcattcctcatcgcagtca
gtcggaaaatcaaagcagccgcttcaaatgcctctgctatgaaggtcaaggatttccttc
tcaagttcgagtatcttcttcttggattggattcttccgagcttcgagtgatccagcaga
aggtccaagaaaaaatcgatcagccgggattggcagagaaggtttttgaaaaccgtcgac
gtccgcgaaattcttcaaggcctgctctttttcattttaaactaattaaaaaattttttt
tttctgttcattttctccctttgttgatttctactttggagctgcacaatggttttcacg
attcgccaatctgtgtcgtcgatgaaaaacgacgagattccgtattggaagcatcgaaca
ctccagcacaagatgagcttcctcagaatcacaaacggactccacatccaccgaaaggat
tcaaattccctcctcgtgtgttgaaaacggtccgatttgaactggaaaatcgtcagctcc
agccgttcacatcattctggaggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAAGATTATCCTATTTCCCGAGCT 666 25 1538 Antisense
CATTCCTCATCGCAGTCAGTCGGAA 404 217 1580 Antisense
CAAAGCAGCCGCTTCAAATGCCTCT 225 191 1608 Antisense
AAGTTCGAGTATCTTCTTCTTGGAT 423 151 1660 Antisense
GATTCTTCCGAGCTTCGAGTGATCC 483 431 1687 Antisense
GACGTCCGCGAAATTCTTCAAGGCC 525 377 1775 Antisense
TTTTCTCCCTTTGTTGATTTCTACT 406 675 1848 Antisense
GGAGCTGCACAATGGTTTTCACGAT 422 521 1875 Antisense
CGATTCGCCAATCTGTGTCGTCGAT 135 249 1896 Antisense
GAATCACAAACGGACTCCACATCCA 471 327 1983 Antisense
AGCCGTTCACATCATTCTGGAGGAC 185 83 2078 Antisense
Sequence Source Affymetrix Proprietary Database