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[177308_at]

Description M195.4 /REP_DB=WormBase Gene ID /WP=CE03517 /TR=Q21564 /GB=CAA91292.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 73      4grp_total > 20      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 43.8
(-3)
A 		81.8
(3)
A 		58.2
(-0.7)
A 		75
(1.9)
A 		38
B 60.4
(3)
A 		56.4
(2.6)
A 		6.9
(-3)
A 		49.2
(1.7)
A 		54
C 56.7
(0.5)
A 		10.1
(-3)
A 		51.4
(0.1)
A 		89.2
(3)
P 		79
avr 53.6
(-0.3) 		49.4
(-1) 		38.8
(-3) 		71.1
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 3.6
(-3)
A 		26.4
(-0.8)
A 		66.1
(3)
M 		41.3
(0.6)
A 		42.6
(0.7)
A 		36.7
(0.2)
A 		63
B 35.4
(-0.3)
A 		30.5
(-1.5)
A 		23.9
(-3)
A 		41.2
(1)
A 		49.6
(3)
M 		28.2
(-2)
A 		26
C 19.6
(-3)
A 		61.9
(3)
A 		34.6
(-0.9)
A 		35.5
(-0.7)
A 		37.6
(-0.4)
A 		32
(-1.2)
M 		42
avr 19.5
(-3) 		39.6
(2.1) 		41.5
(2.5) 		39.3
(2) 		43.3
(3) 		32.3
(0.2) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 16
(1.4)
A 		17.8
(3)
A 		15.2
(0.7)
A 		11
(-3)
A 		7
B 19.2
(3)
A 		13.9
(1.2)
A 		1.6
(-3)
A 		10.5
(0)
A 		18
C 18.8
(3)
A 		2.9
(-3)
A 		17.9
(2.7)
A 		15
(1.6)
P 		16
avr 18
(3) 		11.5
(-3) 		11.6
(-2.9) 		12.2
(-2.4) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 0.7
(-3)
A 		9.4
(0.8)
A 		14.4
(3)
M 		10.5
(1.3)
A 		12.9
(2.3)
A 		8.9
(0.6)
A 		14
B 12.3
(-0.3)
A 		10.2
(-1.2)
A 		5.8
(-3)
A 		10.9
(-0.9)
A 		20.1
(3)
M 		10.5
(-1)
A 		14
C 7.3
(-2.9)
A 		18.9
(3)
A 		8.8
(-2.1)
A 		7.1
(-3)
A 		12.2
(-0.4)
A 		10.7
(-1.2)
M 		12
avr 6.8
(-3) 		12.8
(1.3) 		9.7
(-0.9) 		9.5
(-1) 		15.1
(3) 		10
(-0.7) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177308_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3009
Sequence Type Exemplar sequence
Representative Public ID M195.4 NCBI
Target Description M195.4 /REP_DB=WormBase Gene ID /WP=CE03517 /TR=Q21564 /GB=CAA91292.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063361(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063361 NCBI Caenorhabditis elegans M195.4 (M195.4) mRNA, complete cds. 11/11 None
SNAP00000004362 ENSEMBL cdna:SNAP chromosome:CEL140:II:8376533:8377279:1 11/11 None
GENEFINDER00000004365 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8376533:8377273:1 11/11 None
M195.4 ENSEMBL cdna:known chromosome:CEL140:II:8376533:8377279:1 gene:M195.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8376529-8377276(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M195.4
Entrez Gene 187499 Entrez gene
SwissProt Q21564 EMBL-EBI
WormBase CE03517 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91292 Hypothetical protein M195.4 [Caenorhabditis elegans] ref|NP_495762.1| M195.4 [Caenorhabditis elegans] 4.0E-41
blast CAA91292 Hypothetical protein M195.4 [Caenorhabditis elegans] ref|NP_495762.1| M195.4 [Caenorhabditis elegans] 1.0E-40
blast AAY55902 Hypothetical protein ZK1248.20 [Caenorhabditis elegans] ref|NP_001022498.1| ZK1248.20 [Caenorhabditis elegans] 4.0E-22
blast NP_500694 E03H12.7 [Caenorhabditis elegans] gb|AAC47982.1| Hypothetical protein E03H12.7 [Caenorhabditis elegans] 4.0E-20
Sequence
Target Sequence 
>C. ELEGANS:177308_AT
tgcaaccggacaactacttcatccttgctcagaaaccaatcactgaaccatctgcaagtg
tcatgggtcaagttaaaaatgatgtaaagaaggaagaaccaaaaaatgaagaaaaaaaac
caggcgatgaaaataaaaatcctgctgcagtcggcgctgaaggagctggagccgctgaga
aaccgcaggacaaatcacagaaaccgttcccagagattaagatgccaacggagagtatga
aaaagaagaagaatactgataatgagattgacaaaaggaagaaaatgaagactggtttct
accaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCAACCGGACAACTACTTCATCCT 232 577 14 Antisense
ACTTCATCCTTGCTCAGAAACCAAT 119 103 29 Antisense
GAAACCAATCACTGAACCATCTGCA 440 359 45 Antisense
ATCTGCAAGTGTCATGGGTCAAGTT 316 33 63 Antisense
TAAAAATCCTGCTGCAGTCGGCGCT 196 641 147 Antisense
TGCAGTCGGCGCTGAAGGAGCTGGA 217 577 159 Antisense
GAAGGAGCTGGAGCCGCTGAGAAAC 623 333 172 Antisense
GAGCCGCTGAGAAACCGCAGGACAA 79 387 182 Antisense
GACAAATCACAGAAACCGTTCCCAG 53 367 202 Antisense
ACCGTTCCCAGAGATTAAGATGCCA 300 87 216 Antisense
GAAAATGAAGACTGGTTTCTACCAA 318 353 294 Antisense
Sequence Source Affymetrix Proprietary Database