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[177285_at]

Description Y54F10AL.A /REP_DB=WormBase Gene ID /WP=CE25440 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59558.1
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 26      4grp_highest_8_8 > 56      4grp_high_8_8 > 34      6grp_highest_nonorm > 50      6grp_total > 26      4grp_total > 45      6grp_highest_sub > 01_02      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Cup      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 423
1 2 3 4 range
A 810
(2.3)
P 		846.7
(3)
P 		544
(-3)
P 		565.1
(-2.6)
P 		303
B 775.9
(3)
P 		515.6
(-1.4)
P 		424.2
(-3)
P 		439.7
(-2.7)
P 		352
C 637.4
(3)
P 		592.8
(1.6)
P 		452.5
(-3)
P 		454.7
(-2.9)
P 		185
avr 741.1
(3) 		651.7
(1) 		473.6
(-3) 		486.5
(-2.7) 		268

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 395
1 2 3 4 5 6 range
A 660.5
(2.4)
P 		694.8
(3)
P 		376.7
(-3)
P 		453.3
(-1.6)
P 		382.1
(-2.9)
P 		564.7
(0.5)
P 		318
B 699.5
(3)
P 		573.6
(1.1)
P 		481.9
(-0.3)
P 		304.1
(-3)
P 		464
(-0.6)
P 		356.8
(-2.2)
P 		395
C 623.5
(2.8)
P 		632.2
(3)
P 		550.7
(1.2)
P 		426.6
(-1.6)
P 		367.6
(-3)
P 		366.9
(-3)
P 		265
avr 661.2
(3) 		633.5
(2.4) 		469.8
(-1.3) 		394.7
(-3) 		404.6
(-2.8) 		429.5
(-2.2) 		267
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 218
1 2 3 4 range
A 294.9
(3)
P 		183.8
(-0.1)
P 		142.2
(-1.3)
P 		82.7
(-3)
P 		212
B 247.4
(3)
P 		127.4
(-1.7)
P 		98.3
(-2.8)
P 		93.7
(-3)
P 		154
C 211
(3)
P 		170.4
(1.2)
P 		157.7
(0.6)
P 		76.7
(-3)
P 		134
avr 251.1
(3) 		160.5
(-0.3) 		132.7
(-1.3) 		84.4
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 167
1 2 3 4 5 6 range
A 121.8
(-1.6)
P 		247.1
(3)
P 		82
(-3)
P 		115
(-1.8)
P 		115.3
(-1.8)
P 		137.5
(-1)
P 		165
B 243.4
(3)
P 		190.9
(1.1)
P 		117
(-1.7)
P 		80.5
(-3)
P 		188.5
(1)
P 		133.1
(-1.1)
P 		163
C 231.1
(3)
P 		192.9
(1.4)
P 		140.2
(-0.8)
P 		85.8
(-3)
P 		118.9
(-1.6)
P 		122.8
(-1.5)
P 		145
avr 198.8
(2.4) 		210.3
(3) 		113.1
(-2) 		93.8
(-3) 		140.9
(-0.6) 		131.1
(-1.1) 		117
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177285_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18907
Sequence Type Exemplar sequence
Representative Public ID Y54F10AL.A NCBI
Target Description Y54F10AL.A /REP_DB=WormBase Gene ID /WP=CE25440 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59558.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065165(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065165 NCBI Caenorhabditis elegans EST (Ever Shorter Telomeres) homolog family member (est-1) (est-1) mRNA, complete cds. 11/11 None
GENEFINDER00000039735 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2473869:2486400:1 11/11 None
Y54F10AL.2a.2 ENSEMBL cdna:known chromosome:CEL140:III:2473869:2486774:1 gene:Y54F10AL.2 11/11 None
Y54F10AL.2a.1 ENSEMBL cdna:known chromosome:CEL140:III:2473869:2486609:1 gene:Y54F10AL.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175367 2 1 NM_065165 177285_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2473868-2486400(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y54F10AL.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK27880 Est (ever shorter telomeres) homolog protein 1, isoform a [Caenorhabditis elegans] ref|NP_497566.3| EST (Ever Shorter Telomeres) homolog family member (est-1) [Caenorhabditis elegans] 0.0
blast CAE69472 Hypothetical protein CBG15668 [Caenorhabditis briggsae] 0.0
blast AAK27881 Est (ever shorter telomeres) homolog protein 1, isoform b [Caenorhabditis elegans] ref|NP_497565.1| EST (Ever Shorter Telomeres) homolog family member (est-1) [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:177285_AT
tagtgccaacgacagtgctcgacgagctgcaatacattgccaaactgagcccactcgact
caaaatccaactcagaagctcacgacccggaacgaatttcgaaagcgaaaatcgctgtgg
cttggctcaaagagcaagcaaaacagaagaccggacatttgtacacactgacaacgaccg
gaaaacgactaccaactctatcgatagttagcgaagatttggaaggacacgagatgacga
caaacgacgacatgatcctgaattcggcgctccgatggtcagaatcccttcccggaagca
ctgctccttctgccaccgaaatttcccaaaaatgtgtgctcatcaccggagaccgaggct
tgacgatcaaagcaatcggaaataatttcccgtgccgcggaatttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAGTGCCAACGACAGTGCTCGACGA 677 653 3080 Antisense
AATACATTGCCAAACTGAGCCCACT 1 171 3110 Antisense
AAATCCAACTCAGAAGCTCACGACC 497 117 3142 Antisense
GCTCACGACCCGGAACGAATTTCGA 502 299 3157 Antisense
GAAAATCGCTGTGGCTTGGCTCAAA 671 353 3186 Antisense
GACCGGACATTTGTACACACTGACA 359 383 3228 Antisense
AAACGACTACCAACTCTATCGATAG 235 127 3262 Antisense
GAATTCGGCGCTCCGATGGTCAGAA 115 325 3339 Antisense
TCAGAATCCCTTCCCGGAAGCACTG 51 623 3358 Antisense
TCATCACCGGAGACCGAGGCTTGAC 233 621 3419 Antisense
TAATTTCCCGTGCCGCGGAATTTCA 334 631 3462 Antisense
Sequence Source Affymetrix Proprietary Database