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[177281_at]

Description ZK6.6 /REP_DB=WormBase Gene ID /WP=CE18443 /TR=O61867 /GB=AAG45573.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 42      4grp_total > 41      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 70.5
(-3)
A 		221.2
(3)
P 		213.9
(2.7)
P 		136.1
(-0.4)
A 		151
B 31.8
(-3)
A 		234.7
(3)
P 		203
(2.1)
P 		71
(-1.8)
A 		203
C 21.3
(-3)
A 		193.6
(2.3)
P 		214.9
(3)
P 		104.6
(-0.4)
A 		194
avr 41.2
(-3) 		216.5
(3) 		210.6
(2.8) 		103.9
(-0.9) 		175

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 188
1 2 3 4 5 6 range
A 105.9
(0.9)
A 		105.7
(0.9)
P 		154
(3)
A 		119.7
(1.5)
A 		101.7
(0.8)
A 		13.5
(-3)
A 		141
B 107.3
(-2.1)
A 		169.4
(1.3)
A 		201.2
(3)
P 		112
(-1.9)
A 		91.3
(-3)
A 		107
(-2.1)
A 		110
C 106.2
(-0.9)
A 		140.8
(1.4)
M 		165.7
(3)
A 		101.8
(-1.2)
A 		78.7
(-2.8)
P 		75
(-3)
A 		91
avr 106.5
(-0.7) 		138.6
(1.1) 		173.6
(3) 		111.2
(-0.5) 		90.6
(-1.6) 		65.2
(-3) 		108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 25.7
(-2)
A 		48
(1.7)
P 		55.9
(3)
P 		19.9
(-3)
A 		36
B 10.1
(-3)
A 		58
(3)
P 		47
(1.6)
P 		15.1
(-2.4)
A 		48
C 7
(-3)
A 		55.7
(1.3)
P 		74.9
(3)
P 		17.6
(-2.1)
A 		68
avr 14.3
(-3) 		53.9
(2.3) 		59.3
(3) 		17.5
(-2.6) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 19.5
(-0.2)
A 		37.6
(3)
P 		33.5
(2.3)
A 		30.4
(1.7)
A 		30.7
(1.8)
A 		3.3
(-3)
A 		34
B 37.3
(-1.3)
A 		56.4
(3)
A 		48.9
(1.3)
P 		29.7
(-3)
A 		37.1
(-1.3)
A 		39.9
(-0.7)
A 		27
C 39.4
(2)
A 		43
(3)
M 		42.2
(2.8)
A 		20.5
(-3)
A 		25.5
(-1.7)
P 		25.1
(-1.8)
A 		23
avr 32.1
(-0.6) 		45.7
(3) 		41.5
(1.9) 		26.9
(-1.9) 		31.1
(-0.8) 		22.8
(-3) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177281_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15613
Sequence Type Exemplar sequence
Representative Public ID ZK6.6 NCBI
Target Description ZK6.6 /REP_DB=WormBase Gene ID /WP=CE18443 /TR=O61867 /GB=AAG45573.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070785(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070785 NCBI Caenorhabditis elegans ZK6.6 (ZK6.6) mRNA, complete cds. 10/11 None
GENEFINDER00000041452 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:388550:391360:1 9/11 None
ZK6.6 ENSEMBL cdna:known chromosome:CEL140:V:389588:391360:1 gene:ZK6.6 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041437 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:388847-391360(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK6.6
Entrez Gene 191207 Entrez gene
SwissProt O61867 EMBL-EBI
WormBase CE36079 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG45573 Hypothetical protein ZK6.6 [Caenorhabditis elegans] ref|NP_503186.2| ZK6.6 [Caenorhabditis elegans] 0.0
blast CAE58263 Hypothetical protein CBG01365 [Caenorhabditis briggsae] 0.0
blast AAG45570 Hypothetical protein ZK6.8 [Caenorhabditis elegans] ref|NP_503183.1| ZK6.8 [Caenorhabditis elegans] 1.0E-178
blast CAE58263 Hypothetical protein CBG01365 [Caenorhabditis briggsae] 1.0E-150
blast AAG45570 Hypothetical protein ZK6.8 [Caenorhabditis elegans] ref|NP_503183.1| ZK6.8 [Caenorhabditis elegans] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 7.3E-101
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503186.2 7 42-64,102-124,134-156,169-191,211-233,246-263,267-289
ZK6.6 7 42-64,102-124,134-156,169-191,211-233,246-263,267-289
GENEFINDER00000041452 12 13-33,62-84,91-108,113-135,148-170,200-222,260-282,292-314,327-349,369-391,404-421,425-447
Sequence
Target Sequence 
>C. ELEGANS:177281_AT
aaaatgttcaagttggctccgaccttcttgctcaccggaatgtacacggcattttgggtg
tccatttacccaaccagtctgactttcaacatgcacaactcaaaaatgatatatcttcct
gcaatttacggattcggagttggagttggcgaaactattatgggcattatcatctccaca
ctgagcaagcgcatcaaagatttcggtttgaagccaacgatgctcattggaagtgtgctc
accaccgtcttctgttttgtagctcttctctcaactccgtttgacgctacagtaacccca
agccacgagcaacccttgctctttcagccaaggcgaccaagttacagtatttctgattgc
aataatcggaggaattccctgggctcatgcttcatcttcttcctgtccccattcctcaac
ctctaccactacgtcatcggaatcccattgctctgcgtactgtcgtgtattttatttttc
cgccaggccagacagact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAATGTTCAAGTTGGCTCCGACCT 361 131 730 Antisense
CCTTCTTGCTCACCGGAATGTACAC 695 267 752 Antisense
CATTTACCCAACCAGTCTGACTTTC 430 213 792 Antisense
GTCTGACTTTCAACATGCACAACTC 541 471 806 Antisense
TATGGGCATTATCATCTCCACACTG 30 657 888 Antisense
TCATTGGAAGTGTGCTCACCACCGT 417 619 953 Antisense
CCCTTGCTCTTTCAGCCAAGGCGAC 344 259 1042 Antisense
AATCGGAGGAATTCCCTGGGCTCAT 332 167 1093 Antisense
TACGTCATCGGAATCCCATTGCTCT 170 649 1159 Antisense
GCTCTGCGTACTGTCGTGTATTTTA 681 299 1179 Antisense
ATTTTTCCGCCAGGCCAGACAGACT 602 13 1203 Antisense
Sequence Source Affymetrix Proprietary Database