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[177269_at]

Description Y54E10BR.G /REP_DB=WormBase Gene ID /WP=CE22469 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59554.1
Cluster 6grp_total > 75      4grp_total > 02      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1330
1 2 3 4 range
A 197.8
(-2.4)
P 		144.8
(-3)
A 		266.7
(-1.7)
P 		702.1
(3)
P 		557
B 211.5
(-2.7)
P 		137.2
(-3)
A 		181.5
(-2.8)
A 		1430.8
(3)
P 		1294
C 257.9
(-1.3)
P 		193.7
(-2)
A 		100.8
(-3)
A 		671.6
(3)
P 		571
avr 222.4
(-2.5) 		158.6
(-3) 		183
(-2.8) 		934.8
(3) 		776

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 143.3
(-0.8)
A 		175.8
(2.8)
P 		169.6
(2.1)
A 		124.1
(-3)
A 		177.2
(3)
M 		155.1
(0.5)
A 		53
B 170.9
(-1.3)
P 		203
(0.8)
A 		143
(-3)
M 		238.9
(3)
M 		182
(-0.6)
P 		191.7
(0)
P 		96
C 174.8
(2.3)
P 		77.6
(-3)
P 		175.3
(2.3)
A 		187.3
(3)
P 		125.6
(-0.4)
M 		180.3
(2.6)
P 		110
avr 163
(-0.9) 		152.1
(-3) 		162.6
(-1) 		183.4
(3) 		161.6
(-1.2) 		175.7
(1.5) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 274
1 2 3 4 range
A 72
(0.4)
P 		31.4
(-3)
A 		69.7
(0.2)
P 		102.8
(3)
P 		71
B 67.5
(-2.3)
P 		33.9
(-3)
A 		42.1
(-2.8)
A 		304.9
(3)
P 		271
C 85.4
(0.9)
P 		55.7
(-1.4)
A 		35.1
(-3)
A 		113.2
(3)
P 		78
avr 75
(-1.4) 		40.3
(-3) 		49
(-2.6) 		173.6
(3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 26.4
(-3)
A 		62.5
(3)
P 		36.9
(-1.3)
A 		31.5
(-2.2)
A 		53.5
(1.5)
M 		37.8
(-1.1)
A 		36
B 59.5
(0.8)
P 		67.6
(2)
A 		34.7
(-3)
M 		63.3
(1.4)
M 		74
(3)
P 		71.5
(2.6)
P 		39
C 64.8
(3)
P 		23.7
(-3)
P 		44.6
(0.1)
A 		37.7
(-1)
P 		40.7
(-0.5)
M 		60.4
(2.4)
P 		41
avr 50.2
(0.9) 		51.3
(1.2) 		38.7
(-3) 		44.2
(-1.2) 		56.1
(2.8) 		56.6
(3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177269_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18758
Sequence Type Exemplar sequence
Representative Public ID Y54E10BR.G NCBI
Target Description Y54E10BR.G /REP_DB=WormBase Gene ID /WP=CE22469 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59554.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058689
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000038975 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3015746:3021423:1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_058689 5/11 Cross Hyb Matching Probes A 
SNAP00000038968 5/11 Cross Hyb Matching Probes None
Y54E10BR.4 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3018719-3021423(+) 54.44 96.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59552 Hypothetical protein Y54E10BR.4 [Caenorhabditis elegans] ref|NP_491090.1| Y54E10BR.4 [Caenorhabditis elegans] 1.0E-119
blast CAE60563 Hypothetical protein CBG04192 [Caenorhabditis briggsae] 1.0E-100
blast AAW25869 SJCHGC06824 protein [Schistosoma japonicum] 6.0E-28
Sequence
Target Sequence 
>C. ELEGANS:177269_AT
tttgccatacattttcaattccggcaactttcccatttgccggaaattttcaattccggt
aattttcccatttaccgatttgccggatttgccggaaatttgcgttttttgcaaatttcc
aatttgccgggaattttcaattccagaaagttgccaatttgccggaaattttaaattccg
ccaattttcacatttgccggaaactttcgattccggcaatttgccgatgtgccatacatt
tttaattctgtgtgccgtatccgtcgacgaggatggaaatcaacagcttcatatcaattg
gaatcgaatggcaaatcgacgtggcctagtctacacattggccaatccaaaaggtggaaa
acacgcaacaaatgagcgccttttcgaagatcaaccaatgcctcacatgagaatggcaaa
aattcatatggatccacttgcggatggcccattttctgtgcatgacgtcacttctagatc
tgcaatgctcggagttcgaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGCCATACATTTTCAATTCCGGC 488 667 132 Antisense
GGCAACTTTCCCATTTGCCGGAAAT 177 501 154 Antisense
AATTCCGCCAATTTTCACATTTGCC 662 177 305 Antisense
TTCACATTTGCCGGAAACTTTCGAT 290 641 318 Antisense
ATTTGCCGATGTGCCATACATTTTT 469 17 350 Antisense
TTTTTAATTCTGTGTGCCGTATCCG 620 673 370 Antisense
GCCGTATCCGTCGACGAGGATGGAA 45 285 385 Antisense
TATGGATCCACTTGCGGATGGCCCA 390 655 558 Antisense
GCCCATTTTCTGTGCATGACGTCAC 422 285 578 Antisense
ATGACGTCACTTCTAGATCTGCAAT 468 45 593 Antisense
GATCTGCAATGCTCGGAGTTCGAAC 266 419 608 Antisense
Sequence Source Affymetrix Proprietary Database