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[177265_s_at]

Description Y54E10BR.A /REP_DB=WormBase Gene ID /WP=CE22463 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59548.1
Cluster 6grp_high1_10_10 > 76      6grp_total > 77      4grp_total > 22      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 124.8
(2.9)
A 		3.6
(-3)
A 		48.5
(-0.8)
A 		126.6
(3)
A 		123
B 170.1
(3)
A 		52
(-1.8)
A 		22.6
(-3)
A 		130.5
(1.4)
A 		148
C 100.2
(0.1)
A 		46.9
(-2.3)
A 		31.5
(-3)
A 		162.6
(3)
A 		131
avr 131.7
(2.5) 		34.2
(-3) 		34.2
(-3) 		139.9
(3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 120.3
(-0.5)
A 		78.8
(-3)
A 		132.4
(0.2)
P 		125.4
(-0.2)
P 		106.8
(-1.3)
P 		178
(3)
P 		99
B 117.9
(-3)
P 		183.4
(-0.3)
P 		139.6
(-2.1)
P 		156.3
(-1.4)
P 		191.9
(0.1)
P 		261.3
(3)
P 		143
C 119.3
(-3)
P 		143.2
(-1.9)
P 		129.7
(-2.5)
A 		121.5
(-2.9)
P 		129.2
(-2.5)
P 		244.7
(3)
P 		125
avr 119.2
(-3) 		135.1
(-2.1) 		133.9
(-2.2) 		134.4
(-2.2) 		142.6
(-1.7) 		228
(3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 45.4
(3)
A 		0.8
(-3)
A 		12.7
(-1.4)
A 		18.5
(-0.6)
A 		45
B 54.2
(3)
A 		12.8
(-2.1)
A 		5.2
(-3)
A 		27.8
(-0.2)
A 		49
C 33.2
(3)
A 		13.5
(-2.3)
A 		11
(-3)
A 		27.4
(1.4)
A 		22
avr 44.3
(3) 		9
(-3) 		9.6
(-2.9) 		24.6
(-0.3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 22.2
(-3)
A 		28
(-1.4)
A 		28.8
(-1.1)
P 		31.8
(-0.3)
P 		32.2
(-0.2)
P 		43.3
(3)
P 		21
B 41
(-2.3)
P 		61
(-0.4)
P 		33.9
(-3)
P 		41.4
(-2.3)
P 		78
(1.2)
P 		97.4
(3)
P 		64
C 44.2
(-0.9)
P 		43.7
(-1)
P 		33
(-2.1)
A 		24.4
(-3)
P 		41.8
(-1.2)
P 		81.9
(3)
P 		58
avr 35.8
(-2.4) 		44.2
(-1.3) 		31.9
(-3) 		32.5
(-2.9) 		50.7
(-0.3) 		74.2
(3) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177265_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18693
Sequence Type Exemplar sequence
Representative Public ID Y54E10BR.A NCBI
Target Description Y54E10BR.A /REP_DB=WormBase Gene ID /WP=CE22463 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59548.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058695(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058695 NCBI Caenorhabditis elegans Y54E10BR.8 (Y54E10BR.8) mRNA, complete cds. 11/11 None
SNAP00000038973 ENSEMBL cdna:SNAP chromosome:CEL140:I:3035239:3059072:-1 11/11 None
GENEFINDER00000038977 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3052129:3059072:-1 9/11 None
Y54E10BR.8 ENSEMBL cdna:known chromosome:CEL140:I:3052066:3059147:-1 gene:Y54E10BR.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3052128-3058969(-) 99.17 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E10BR.8
Entrez Gene 171880 Entrez gene
SwissProt Q9N3C6 EMBL-EBI
WormBase CE27284 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF59548 Hypothetical protein Y54E10BR.8 [Caenorhabditis elegans] ref|NP_491096.1| Y54E10BR.8 [Caenorhabditis elegans] 0.0
blast AAF59549 Modulation of locomotion defective protein 5 [Caenorhabditis elegans] ref|NP_491095.3| Modulation Of locomotion Defective family member (mod-5) [Caenorhabditis elegans] 0.0
blast AAK84832 serotonin reuptake transporter [Caenorhabditis elegans] 0.0
blast CAE60568 Hypothetical protein CBG04197 [Caenorhabditis briggsae] 0.0
blast CAE60570 Hypothetical protein CBG04199 [Caenorhabditis briggsae] 1.0E-151
blast AAF59548 Hypothetical protein Y54E10BR.8 [Caenorhabditis elegans] ref|NP_491096.1| Y54E10BR.8 [Caenorhabditis elegans] 1.0E-128
blast CAE60570 Hypothetical protein CBG04199 [Caenorhabditis briggsae] 1.0E-87
blast XP_531880 PREDICTED: similar to Zinc finger protein 208 [Canis familiaris] 1.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 1.4E-4
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 4.1E-6
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 3.5E-6
Pfam IPR000175 EMBL-EBI Sodium:neurotransmitter symporter 1.0E-126
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 4.1E-6
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 1.1E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000038973 11 503-525,546-568,638-660,669-691,714-736,756-778,810-832,852-874,884-906,923-945,965-987
Sequence
Target Sequence 
>C. ELEGANS:177265_S_AT
aatgtggtggtcaacgcgacgcaacgggattctccggaacaagaggattgttccacgagc
ggagtcgcatttgcagatggtcagacgaatagtggaaaaatgggaagattcagttgtgat
cggtgttctaggacatttaaataccaatccaaactggatgaacatcgtcgaacacatctc
ggagtaaaaccatttcaatgtcactactgtacccgccaattctctcaacgtggagctctt
aaaacacacatgagattacatacaggagaacgtccatttgtatgtcaatgggaatgtgga
aaacaatttgcttcttcatctgcaaaaagtcatcatgaaaagacacatagtggagagcga
ccgtatatttgtaatgtttgtggaaaaagtttcacgaagaattcacatgttattcggcat
cttaagaacattcacaatcgagaactggctcaacaggatgcaatgctacgtggcgacgat
gtccaatcgaccgatgaatctttaaatattggctccgcgcgaagtcagatgtccgatata
gagccacgtggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGTGGTGGTCAACGCGACGCAAC 72 163 592 Antisense
CGACGCAACGGGATTCTCCGGAACA 204 251 608 Antisense
GTTCCACGAGCGGAGTCGCATTTGC 321 441 641 Antisense
TCAGTTGTGATCGGTGTTCTAGGAC 697 623 701 Antisense
GAACATCGTCGAACACATCTCGGAG 323 351 751 Antisense
GAAAACAATTTGCTTCTTCATCTGC 522 355 890 Antisense
AATTCACATGTTATTCGGCATCTTA 60 177 991 Antisense
GGATGCAATGCTACGTGGCGACGAT 269 513 1047 Antisense
TGGCGACGATGTCCAATCGACCGAT 600 543 1062 Antisense
CTCCGCGCGAAGTCAGATGTCCGAT 178 235 1104 Antisense
GATGTCCGATATAGAGCCACGTGGC 376 409 1119 Antisense
Sequence Source Affymetrix Proprietary Database