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[177264_at]

Description M110.3 /REP_DB=WormBase Gene ID /WP=CE02276 /TR=Q21530 /GB=CAA90260.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 92      6grp_total > 97      4grp_total > 12      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 236.6
(-2.1)
P 		241
(-1.9)
P 		218.7
(-3)
P 		337.1
(3)
P 		118
B 296.7
(0.5)
P 		283.1
(-0.3)
P 		232.7
(-3)
P 		343.5
(3)
P 		111
C 276.5
(-0.4)
P 		247.9
(-2.7)
P 		244
(-3)
P 		317.9
(3)
P 		74
avr 269.9
(-0.7) 		257.3
(-1.5) 		231.8
(-3) 		332.8
(3) 		101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 386
1 2 3 4 5 6 range
A 272.4
(-1.8)
P 		206.1
(-3)
P 		273.6
(-1.8)
P 		320.9
(-1)
P 		459.6
(1.5)
P 		546.3
(3)
P 		340
B 243.3
(-3)
P 		245.7
(-3)
P 		272.3
(-2.5)
P 		365.4
(-0.9)
P 		468
(0.9)
P 		592.2
(3)
P 		349
C 244.6
(-3)
P 		269.2
(-2.5)
P 		253.8
(-2.8)
P 		258.8
(-2.7)
P 		343.6
(-1)
P 		535.2
(3)
P 		291
avr 253.4
(-2.8) 		240.3
(-3) 		266.6
(-2.5) 		315
(-1.6) 		423.7
(0.5) 		557.9
(3) 		318
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 86.1
(3)
P 		52.3
(-2.5)
P 		57.2
(-1.7)
P 		49.3
(-3)
P 		37
B 94.6
(3)
P 		69.9
(-0.6)
P 		53.9
(-3)
P 		73.2
(-0.2)
P 		41
C 91.5
(3)
P 		71.3
(-0.2)
P 		85
(2)
P 		53.6
(-3)
P 		38
avr 90.7
(3) 		64.5
(-1.9) 		65.4
(-1.7) 		58.7
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 50.2
(-3)
P 		73.3
(-1.4)
P 		59.6
(-2.4)
P 		81.4
(-0.9)
P 		138.7
(3)
P 		133
(2.6)
P 		89
B 84.7
(-2.3)
P 		81.8
(-2.4)
P 		66.1
(-3)
P 		96.8
(-1.8)
P 		190.1
(1.8)
P 		220.8
(3)
P 		155
C 90.6
(-1.2)
P 		82.2
(-1.6)
P 		64.6
(-2.4)
P 		52
(-3)
P 		111.2
(-0.2)
P 		179.2
(3)
P 		127
avr 75.2
(-2.4) 		79.1
(-2.2) 		63.4
(-3) 		76.7
(-2.3) 		146.7
(1.4) 		177.7
(3) 		114
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177264_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3330
Sequence Type Exemplar sequence
Representative Public ID M110.3 NCBI
Target Description M110.3 /REP_DB=WormBase Gene ID /WP=CE02276 /TR=Q21530 /GB=CAA90260.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063327(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063327 NCBI Caenorhabditis elegans M110.3 (M110.3) mRNA, complete cds. 11/11 None
SNAP00000006400 ENSEMBL cdna:SNAP chromosome:CEL140:II:8214720:8216349:-1 11/11 None
GENEFINDER00000006411 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8214097:8216349:-1 10/11 None
M110.3 ENSEMBL cdna:known chromosome:CEL140:II:8214720:8216349:-1 gene:M110.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8214716-8216346(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M110.3
Entrez Gene 187472 Entrez gene
SwissProt Q21530 EMBL-EBI
WormBase CE02276 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90260 Hypothetical protein M110.3 [Caenorhabditis elegans] ref|NP_495728.1| M110.3 [Caenorhabditis elegans] 1.0E-112
blast CAA90260 Hypothetical protein M110.3 [Caenorhabditis elegans] ref|NP_495728.1| M110.3 [Caenorhabditis elegans] 1.0E-92
blast CAE57704 Hypothetical protein CBG00710 [Caenorhabditis briggsae] 4.0E-92
blast CAE57704 Hypothetical protein CBG00710 [Caenorhabditis briggsae] 4.0E-76
blast EAA04914 ENSANGP00000016030 [Anopheles gambiae str. PEST] ref|XP_309240.2| ENSANGP00000016030 [Anopheles gambiae str. PEST] 1.0E-11
Protein Domains
Database ID Description E-Value
scop a.2.8.Eukaryotic DNA topoisomerase I, dispensable insert domain All alpha proteins; Long alpha-hairpin; Eukaryotic DNA topoisomerase I, dispensable insert domain; Eukaryotic DNA topoisomerase I, dispensable insert domain 2.29999995231628
Pfam IPR001841 EMBL-EBI Zn-finger, RING 1.0E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000006411 1 211-233
Sequence
Target Sequence 
>C. ELEGANS:177264_AT
aatgcgttaagcttcgttcatccgtgtcgatgtcgaggatctattcactgggttcatcat
caatgcctggccatgtggttttcgaaagcgaatgctgtacaacaagttatgtgtattcag
tgccaaacacgatatcaaaaacaattaacactcaaatcatggaaatcctggacaattcct
cgctttggaatcgataagtttggcttgctggaaattactgttgatttatggataacatgg
agaactgtatccggtttcgttggaatgatgaacggaacaaagggaattttccgagagata
attctatgcaccatgtggaaatgttttgtagcatctggaaggaggttttgctactacgga
aatctcggactaagacttgcttcttcgattttccgagttagtattgatgactatgaagaa
agacgagaaatcaaggcacgacttccaaccgatatgcactggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCGTTAAGCTTCGTTCATCCGT 293 167 88 Antisense
CGTTCATCCGTGTCGATGTCGAGGA 449 247 102 Antisense
GTCGAGGATCTATTCACTGGGTTCA 84 475 119 Antisense
TGGGTTCATCATCAATGCCTGGCCA 116 555 136 Antisense
AATGCCTGGCCATGTGGTTTTCGAA 41 167 149 Antisense
GAAATCCTGGACAATTCCTCGCTTT 117 363 249 Antisense
GAGAACTGTATCCGGTTTCGTTGGA 157 393 327 Antisense
GAGGTTTTGCTACTACGGAAATCTC 191 401 429 Antisense
ACTTGCTTCTTCGATTTTCCGAGTT 202 105 462 Antisense
AGAAATCAAGGCACGACTTCCAACC 630 71 513 Antisense
GACTTCCAACCGATATGCACTGGGA 322 365 527 Antisense
Sequence Source Affymetrix Proprietary Database