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[177258_at]

Description M162.3 /REP_DB=WormBase Gene ID /WP=CE18893 /TR=Q9XUC8 /GB=CAB05255.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 02      6grp_cgc > 91      6grp_cgc_sub > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 211
1 2 3 4 range
A 5
(-2.9)
A 		2.7
(-3)
A 		6.7
(-2.9)
A 		212
(3)
P 		209
B 26.7
(-2.2)
A 		3.1
(-3)
A 		6.9
(-2.9)
A 		173.8
(3)
P 		171
C 3.1
(-2.9)
A 		2.3
(-2.9)
A 		1.2
(-3)
A 		96
(3)
P 		95
avr 11.6
(-2.7) 		2.7
(-3) 		4.9
(-2.9) 		160.6
(3) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 5.1
(-2.8)
A 		3.4
(-3)
A 		66.8
(3)
A 		4
(-2.9)
A 		4.8
(-2.9)
A 		4.9
(-2.9)
A 		63
B 1.8
(-3)
A 		24
(3)
A 		6.9
(-1.6)
A 		2.3
(-2.9)
A 		4.6
(-2.2)
A 		19.4
(1.8)
A 		22
C 2.8
(-2.5)
A 		4.3
(-1.4)
A 		3.4
(-2)
A 		4.7
(-1.1)
A 		2.1
(-3)
A 		10.2
(3)
A 		8
avr 3.2
(-3) 		10.6
(-1) 		25.7
(3) 		3.7
(-2.9) 		3.8
(-2.8) 		11.5
(-0.8) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 1.8
(-2.8)
A 		0.6
(-3)
A 		1.8
(-2.8)
A 		31
(3)
P 		30
B 8.5
(-1.7)
A 		0.8
(-3)
A 		1.6
(-2.9)
A 		37
(3)
P 		36
C 1
(-2.8)
A 		0.7
(-2.9)
A 		0.4
(-3)
A 		16.2
(3)
P 		16
avr 3.8
(-2.3) 		0.7
(-3) 		1.3
(-2.9) 		28.1
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 0.9
(-3)
A 		1.2
(-2.9)
A 		14.6
(3)
A 		1
(-3)
A 		1.5
(-2.7)
A 		1.2
(-2.9)
A 		14
B 0.6
(-3)
A 		8
(3)
A 		1.7
(-2.1)
A 		0.6
(-3)
A 		1.9
(-1.9)
A 		7.2
(2.4)
A 		7
C 1
(-2.3)
A 		1.3
(-1.7)
A 		0.9
(-2.6)
A 		0.9
(-2.6)
A 		0.7
(-3)
A 		3.4
(3)
A 		3
avr 0.8
(-3) 		3.5
(0.3) 		5.7
(3) 		0.8
(-3) 		1.4
(-2.3) 		3.9
(0.8) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177258_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12602
Sequence Type Exemplar sequence
Representative Public ID M162.3 NCBI
Target Description M162.3 /REP_DB=WormBase Gene ID /WP=CE18893 /TR=Q9XUC8 /GB=CAB05255.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075433(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075433 NCBI Caenorhabditis elegans Serpentine Receptor, class T family member (srt-45) (srt-45) mRNA, complete cds. 11/11 A 
M162.3 ENSEMBL cdna:known chromosome:CEL140:V:19782127:19783959:1 gene:M162.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000003508 1/11 Cross Hyb Matching Probes None
GENEFINDER00000003570 1/11 Cross Hyb Matching Probes None
GENEFINDER00000003580 1/11 Cross Hyb Matching Probes None
SNAP00000032749 8/11 Cross Hyb Matching Probes None
GENEFINDER00000032756 8/11 Cross Hyb Matching Probes None
Y57G11C.28 1/11 Cross Hyb Matching Probes None
Y57G11C.30 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14929614-14941839(+) 90.33 90.33
chrV:19782126-19783959(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srt-45
Ensembl M162.3
Entrez Gene 187487 Entrez gene
SwissProt Q9XUC8 EMBL-EBI
WormBase CE18893 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05255 Hypothetical protein M162.3 [Caenorhabditis elegans] ref|NP_507834.1| Serpentine Receptor, class T family member (srt-45) [Caenorhabditis elegans] 0.0
blast T23774 hypothetical protein M162.4 - Caenorhabditis elegans 1.0E-143
blast CAE68187 Hypothetical protein CBG13847 [Caenorhabditis briggsae] 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 1.1E-82
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507834.1 7 36-58,71-93,108-130,151-173,201-223,244-266,270-292
M162.3 7 36-58,71-93,108-130,151-173,201-223,244-266,270-292
Sequence
Target Sequence 
>C. ELEGANS:177258_AT
ctacatttgttggtttacacccacaactgcgtataattctgtatttatggcatggattcc
tgatccattatctgaggaagtacccagtaaagaggcagctgcaatgtacaaacacaccat
tctaccgtggaacaattggatttttgtggcatgcatgttcattttattttctgtgtattt
tataatggtaaaacggatggcgaagggacagaaatcaaaggcttccagatcaattttcat
tcaatgctccataatttgcttcttcaataccggaactgctttggtctacaacgcgctagc
aattgtaacccctgctgaatggattcttgcatttggacaaatatgttggacatgtaatca
tgcaagtcctgctattatatatgtctctttaaatagcactatccgcagggaattcttgaa
aattgtgttccgaaacactttggcaccgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACATTTGTTGGTTTACACCCACA 203 227 498 Antisense
ACACCCACAACTGCGTATAATTCTG 87 111 514 Antisense
GAGGCAGCTGCAATGTACAAACACA 345 405 589 Antisense
CAAACACACCATTCTACCGTGGAAC 475 189 606 Antisense
TTTGCTTCTTCAATACCGGAACTGC 578 667 752 Antisense
ACCGGAACTGCTTTGGTCTACAACG 152 87 766 Antisense
CAACGCGCTAGCAATTGTAACCCCT 49 187 786 Antisense
GTAACCCCTGCTGAATGGATTCTTG 103 463 802 Antisense
GTAATCATGCAAGTCCTGCTATTAT 286 463 851 Antisense
ATAGCACTATCCGCAGGGAATTCTT 613 21 890 Antisense
GTGTTCCGAAACACTTTGGCACCGG 569 485 922 Antisense
Sequence Source Affymetrix Proprietary Database