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[177256_at]

Description Y71G10AR.D /REP_DB=WormBase Gene ID /WP=CE25576 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60870.1
Cluster 6grp_total > 25      4grp_total > 30      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 119.8
(3)
A 		32
(-2.1)
A 		17.3
(-3)
A 		84.1
(0.9)
A 		103
B 116.9
(3)
A 		44.7
(-1.3)
A 		15.1
(-3)
A 		115.2
(2.9)
A 		102
C 98.3
(3)
A 		65.3
(0.8)
A 		8.2
(-3)
A 		91.1
(2.5)
A 		90
avr 111.7
(3) 		47.3
(-0.9) 		13.5
(-3) 		96.8
(2.1) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 72.7
(3)
A 		37.3
(-0.3)
A 		21.1
(-1.7)
A 		7.5
(-3)
A 		10.2
(-2.8)
A 		14.6
(-2.3)
A 		65
B 52.9
(3)
A 		48.5
(1.9)
A 		44.4
(0.9)
A 		39.6
(-0.2)
A 		31.8
(-2.1)
A 		28.3
(-3)
A 		25
C 50.9
(0.5)
A 		78.7
(3)
A 		35.1
(-1)
A 		33.5
(-1.1)
A 		12.5
(-3)
A 		48.7
(0.3)
A 		66
avr 58.8
(3) 		54.8
(2.4) 		33.5
(-0.7) 		26.9
(-1.7) 		18.2
(-3) 		30.5
(-1.2) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 43.6
(3)
A 		6.9
(-2.6)
A 		4.5
(-3)
A 		12.3
(-1.8)
A 		39
B 37.3
(3)
A 		11
(-1.7)
A 		3.5
(-3)
A 		24.5
(0.7)
A 		34
C 32.5
(3)
A 		18.8
(0.2)
A 		2.9
(-3)
A 		15.4
(-0.5)
A 		30
avr 37.8
(3) 		12.2
(-1.5) 		3.6
(-3) 		17.4
(-0.6) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 13.4
(3)
A 		13.3
(2.9)
A 		4.6
(-1.6)
A 		1.9
(-3)
A 		3.1
(-2.4)
A 		3.6
(-2.1)
A 		12
B 18.4
(3)
A 		16.1
(1.3)
A 		10.8
(-2.8)
A 		10.5
(-3)
A 		12.9
(-1.2)
A 		10.5
(-3)
A 		8
C 18.9
(1.5)
A 		24
(3)
A 		8.9
(-1.5)
A 		6.7
(-2.2)
A 		4
(-3)
A 		16.3
(0.7)
A 		20
avr 16.9
(2.5) 		17.8
(3) 		8.1
(-2.1) 		6.4
(-3) 		6.7
(-2.8) 		10.1
(-1.1) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177256_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19244
Sequence Type Exemplar sequence
Representative Public ID Y71G10AR.D NCBI
Target Description Y71G10AR.D /REP_DB=WormBase Gene ID /WP=CE25576 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60870.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067775(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067775 NCBI Caenorhabditis elegans Y71G10AR.1 (Y71G10AR.1) mRNA, complete cds. 11/11 None
GENEFINDER00000002961 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2198267:2208830:1 10/11 None
Y71G10AR.1 ENSEMBL cdna:known chromosome:CEL140:IV:2206369:2208830:1 gene:Y71G10AR.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2206374-2208836(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71G10AR.1
Entrez Gene 190593 Entrez gene
SwissProt Q9N2Y6 EMBL-EBI
WormBase CE25576 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.742.1.S1_AT similar to P3 protein cfa
DROSOPHILA_2:1625902_AT dm
DROSGENOME1:145119_AT dm
HG-U133A:204928_S_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 hs
HG-U133_PLUS_2:204928_S_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 hs
HG-FOCUS:204928_S_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 hs
HG-U133A_2:204928_S_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 hs
HUGENEFL:X12458_RNA1_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 hs
U133_X3P:G10938005_3P_A_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 hs
HG-U95AV2:40360_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 hs
MU11KSUBB:MSA.1266.0_S_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 mm
MG-U74AV2:103218_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 mm
MOE430A:1451227_A_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 mm
MOUSE430A_2:1451227_A_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 mm
MOUSE430_2:1451227_A_AT solute carrier family 10 (sodium/bile acid cotransporter family), member 3 mm
RG-U34B:RC_AA957554_AT similar to protein P3 rn
RAE230A:1377359_AT similar to protein P3 rn
RAT230_2:1377359_AT similar to protein P3 rn
Protein Similarities
Method ID Description E-Value
blast CAE68204 Hypothetical protein CBG13872 [Caenorhabditis briggsae] 0.0
blast AAF60870 Hypothetical protein Y71G10AR.1 [Caenorhabditis elegans] ref|NP_500176.1| Y71G10AR.1 [Caenorhabditis elegans] 1.0E-115
blast AAF60870 Hypothetical protein Y71G10AR.1 [Caenorhabditis elegans] ref|NP_500176.1| Y71G10AR.1 [Caenorhabditis elegans] 1.0E-99
blast CAE68204 Hypothetical protein CBG13872 [Caenorhabditis briggsae] 2.0E-89
blast AAF48454 CG11655-PA [Drosophila melanogaster] gb|AAL48132.1| RH04535p [Drosophila melanogaster] ref|NP_573024.1| CG11655-PA [Drosophila melanogaster] 3.0E-52
blast AAF48454 CG11655-PA [Drosophila melanogaster] gb|AAL48132.1| RH04535p [Drosophila melanogaster] ref|NP_573024.1| CG11655-PA [Drosophila melanogaster] 8.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR002657 EMBL-EBI Bile acid:sodium symporter 3.3E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500176.1 4 21-38,53-75,82-104,146-168
Y71G10AR.1 4 21-38,53-75,82-104,146-168
GENEFINDER00000002961 8 269-288,308-330,337-359,369-391,446-468,473-495,502-521,536-558
Sequence
Target Sequence 
>C. ELEGANS:177256_AT
aaattgctagtcaacttgtcaccctgattcttccacttctcatcggaatagccatcaaga
agttcaagccaacgtgggccgcaaaggcccggaagattatgcggccatttgtgatcttcg
ttatgatttttgtggtgatcttcggatcgcttaccaatctctacatgttccgaatgctca
ccggcccggcgttgatcggaggattggctctcccgtggtgtggctttatgttcggctgtt
tcgcggctcttttaacaaaacgaaagcctgaagatgtgactgcaattgccgtagaaactg
gaattcagaatactggaattgcgattctcttgttaaaagcatcttttggtcaaccggatg
ctgatattggatcacttattcctgtaattgtggctgcattcactccgggaccacttttac
tcggcgcagctgtacatttgacatttaaatggatgagaaatcgaaggaatatgaagtgtc
ccgacacggattccatcgaaaaacaaggagttgagctcatcgatgcctcaaagctcacgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATTGCTAGTCAACTTGTCACCCT 647 115 71 Antisense
TCCACTTCTCATCGGAATAGCCATC 370 589 102 Antisense
AAGTTCAAGCCAACGTGGGCCGCAA 524 153 130 Antisense
ATTATGCGGCCATTTGTGATCTTCG 311 11 166 Antisense
GTGATCTTCGGATCGCTTACCAATC 466 483 205 Antisense
CTTACCAATCTCTACATGTTCCGAA 194 223 220 Antisense
TGATCGGAGGATTGGCTCTCCCGTG 1 567 263 Antisense
TGTGGCTTTATGTTCGGCTGTTTCG 484 553 289 Antisense
TTACTCGGCGCAGCTGTACATTTGA 447 681 487 Antisense
GAAGTGTCCCGACACGGATTCCATC 641 335 543 Antisense
CTCATCGATGCCTCAAAGCTCACGG 616 227 586 Antisense
Sequence Source Affymetrix Proprietary Database