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[177254_at]

Description ZC15.8 /REP_DB=WormBase Gene ID /WP=CE16689 /TR=O18271 /GB=CAB07715.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 22      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 14      6grp_total > 22      4grp_total > 52      6grp_highest_sub > De      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4972
1 2 3 4 range
A 3894.8
(0.9)
P 		5644.9
(3)
P 		3994.3
(1)
P 		673.1
(-3)
P 		4972
B 4823.2
(2.4)
P 		5242.5
(3)
P 		4300.7
(1.7)
P 		752.5
(-3)
P 		4490
C 4244.9
(1.9)
P 		5061.3
(3)
P 		3566.2
(0.9)
P 		698.9
(-3)
P 		4362
avr 4321
(1.7) 		5316.2
(3) 		3953.7
(1.2) 		708.2
(-3) 		4608

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3685
1 2 3 4 5 6 range
A 5024.4
(3)
P 		5004.4
(3)
P 		4409.5
(2)
P 		3062.5
(-0.2)
P 		2756.8
(-0.7)
P 		1388.9
(-3)
P 		3636
B 4414
(2.7)
P 		4494.6
(2.8)
P 		4575.1
(3)
P 		2694.5
(-0.5)
P 		2902.9
(-0.2)
P 		1393.7
(-3)
P 		3181
C 4376.8
(2.9)
P 		4364.6
(2.9)
P 		4416
(3)
P 		2786.5
(-0.2)
P 		2942.4
(0.1)
P 		1339
(-3)
P 		3077
avr 4605.1
(3) 		4621.2
(3) 		4466.9
(2.7) 		2847.8
(-0.3) 		2867.4
(-0.2) 		1373.9
(-3) 		3247
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1440
1 2 3 4 range
A 1418.1
(3)
P 		1225.3
(2.1)
P 		1044.2
(1.3)
P 		98.5
(-3)
P 		1320
B 1538.1
(3)
P 		1294.8
(1.9)
P 		996.6
(0.6)
P 		160.4
(-3)
P 		1378
C 1405.2
(2.8)
P 		1455.2
(3)
P 		1242.9
(2)
P 		117.8
(-3)
P 		1337
avr 1453.8
(3) 		1325.1
(2.4) 		1094.6
(1.4) 		125.6
(-3) 		1328

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1442
1 2 3 4 5 6 range
A 926.2
(-0.6)
P 		1779.8
(3)
P 		960.3
(-0.4)
P 		776.7
(-1.2)
P 		831.9
(-0.9)
P 		338.1
(-3)
P 		1442
B 1535.9
(3)
P 		1496.1
(2.8)
P 		1111
(0.5)
P 		713.7
(-1.9)
P 		1179.4
(0.9)
P 		519.7
(-3)
P 		1016
C 1622.3
(3)
P 		1331.8
(1.5)
P 		1124
(0.5)
P 		560.4
(-2.4)
P 		952.1
(-0.4)
P 		448.2
(-3)
P 		1174
avr 1361.5
(2) 		1535.9
(3) 		1065.1
(0.4) 		683.6
(-1.6) 		987.8
(0) 		435.3
(-3) 		1101
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177254_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14441
Sequence Type Exemplar sequence
Representative Public ID ZC15.8 NCBI
Target Description ZC15.8 /REP_DB=WormBase Gene ID /WP=CE16689 /TR=O18271 /GB=CAB07715.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075510(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075510 NCBI Caenorhabditis elegans Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-94) (pqn-94) mRNA, complete cds. 11/11 None
SNAP00000015574 ENSEMBL cdna:SNAP chromosome:CEL140:V:20276780:20288136:-1 11/11 None
GENEFINDER00000015583 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20276750:20287583:-1 11/11 None
ZC15.8.1 ENSEMBL cdna:known chromosome:CEL140:V:20276709:20277912:-1 gene:ZC15.8 11/11 None
ZC15.8.2 ENSEMBL cdna:known chromosome:CEL140:V:20276721:20277914:-1 gene:ZC15.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20276749-20277905(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pqn-94
Ensembl ZC15.8
Entrez Gene 180328 Entrez gene
SwissProt O18271 EMBL-EBI
WormBase CE16689 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07714 Hypothetical protein ZC15.7 [Caenorhabditis elegans] ref|NP_507912.1| ZC15.7 [Caenorhabditis elegans] 0.0
blast CAE67437 Hypothetical protein CBG12929 [Caenorhabditis briggsae] 0.0
blast AAQ09555 inv-like protein [Hemicentrotus pulcherrimus] 7.0E-50
blast CAA09388 inversin [Mus musculus] 2.0E-44
blast CAB07715 Hypothetical protein ZC15.8 [Caenorhabditis elegans] ref|NP_507911.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-94) [Caenorhabditis elegans] 5.0E-15
blast CAE67420 Hypothetical protein CBG12908 [Caenorhabditis briggsae] 2.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR002110 EMBL-EBI Ankyrin 0.11
Pfam IPR002110 EMBL-EBI Ankyrin 0.34
Pfam IPR002110 EMBL-EBI Ankyrin 0.12
Pfam IPR002110 EMBL-EBI Ankyrin 1.2E-9
Pfam IPR002110 EMBL-EBI Ankyrin 7.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 1.6E-4
Pfam IPR002110 EMBL-EBI Ankyrin 9.0E-9
Pfam IPR002110 EMBL-EBI Ankyrin 1.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 34.0
Pfam IPR002110 EMBL-EBI Ankyrin 0.61
Pfam IPR002110 EMBL-EBI Ankyrin 2.7E-5
Pfam IPR002110 EMBL-EBI Ankyrin 0.11
Pfam IPR002110 EMBL-EBI Ankyrin 0.34
Pfam IPR002110 EMBL-EBI Ankyrin 0.12
Pfam IPR002110 EMBL-EBI Ankyrin 1.2E-9
Pfam IPR002110 EMBL-EBI Ankyrin 7.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 1.6E-4
Pfam IPR002110 EMBL-EBI Ankyrin 1.4E-5
Pfam IPR002110 EMBL-EBI Ankyrin 1.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 34.0
Pfam IPR002110 EMBL-EBI Ankyrin 0.61
Pfam IPR002110 EMBL-EBI Ankyrin 2.7E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000015583 5 13-35,263-285,661-683,718-735,805-827
Sequence
Target Sequence 
>C. ELEGANS:177254_AT
cgtattcgcccaataccaacagcaacagtaccagcaacagccatacaacaatcagcagta
ttcaaacaatcaataccaaaccacccgaggatacaatcaacaacaacagtacggacaaac
taaccaattcggacaaacgacaactccacgatacactagccaatcaatgtacggaacaac
acaatcgggccgacaattcgatcaggccggaaatcaggtgttcttcaactctgtcggaca
atcctcagcagttctttcgctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTATTCGCCCAATACCAACAGCAA 455 247 51 Antisense
AGTACCAGCAACAGCCATACAACAA 335 61 77 Antisense
TCAATACCAAACCACCCGAGGATAC 352 633 120 Antisense
ATTCGGACAAACGACAACTCCACGA 170 9 177 Antisense
CAACTCCACGATACACTAGCCAATC 18 189 191 Antisense
GGAACAACACAATCGGGCCGACAAT 122 529 223 Antisense
CGGGCCGACAATTCGATCAGGCCGG 96 245 236 Antisense
GATCAGGCCGGAAATCAGGTGTTCT 424 421 250 Antisense
TCAGGTGTTCTTCAACTCTGTCGGA 645 621 264 Antisense
ACTCTGTCGGACAATCCTCAGCAGT 486 99 278 Antisense
ACAATCCTCAGCAGTTCTTTCGCTG 321 113 288 Antisense
Sequence Source Affymetrix Proprietary Database