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[177253_at]

Description R186.1 /REP_DB=WormBase Gene ID /WP=CE12870 /TR=Q22009 /GB=CAB01439.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 46      6grp_high1_10_10 > 64      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 80      6grp_total > 54      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 206
1 2 3 4 range
A 137
(-1.2)
P 		194.8
(0.5)
P 		75.3
(-3)
P 		281.5
(3)
P 		206
B 174.9
(-2.2)
P 		159.1
(-3)
P 		160.2
(-2.9)
P 		276.8
(3)
P 		118
C 159.9
(-1.9)
P 		144.5
(-3)
P 		147.3
(-2.8)
P 		230.9
(3)
P 		86
avr 157.3
(-1.7) 		166.1
(-1.3) 		127.6
(-3) 		263.1
(3) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 234.9
(3)
P 		194.2
(0.4)
P 		231.2
(2.8)
P 		208.9
(1.4)
P 		139.6
(-3)
P 		168.6
(-1.2)
P 		95
B 185.6
(1.1)
P 		125.8
(-3)
P 		192.2
(1.6)
P 		209.6
(2.8)
P 		146
(-1.6)
P 		212.9
(3)
P 		87
C 197.6
(-0.4)
P 		160.5
(-1.9)
P 		282.6
(3)
P 		134
(-3)
P 		167.2
(-1.7)
P 		233.9
(1)
P 		149
avr 206
(0.9) 		160.2
(-2.3) 		235.3
(3) 		184.2
(-0.6) 		150.9
(-3) 		205.1
(0.9) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 49.9
(3)
P 		42.3
(1.5)
P 		19.7
(-3)
P 		41.2
(1.3)
P 		30
B 55.8
(2.1)
P 		39.3
(-2.4)
P 		37.1
(-3)
P 		59
(3)
P 		22
C 52.9
(3)
P 		41.5
(-1.9)
P 		51.4
(2.4)
P 		38.9
(-3)
P 		14
avr 52.9
(3) 		41
(-1.3) 		36.1
(-3) 		46.4
(0.7) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 43.3
(-2.5)
P 		69.1
(3)
P 		50.3
(-1)
P 		53
(-0.4)
P 		42.1
(-2.8)
P 		41
(-3)
P 		28
B 64.6
(0.6)
P 		41.9
(-3)
P 		46.7
(-2.2)
P 		55.5
(-0.8)
P 		59.3
(-0.2)
P 		79.4
(3)
P 		38
C 73.2
(2.4)
P 		49
(-0.4)
P 		71.9
(2.3)
P 		26.9
(-3)
P 		54.1
(0.2)
P 		78.3
(3)
P 		51
avr 60.4
(1.4) 		53.3
(-0.7) 		56.3
(0.2) 		45.1
(-3) 		51.8
(-1.1) 		66.2
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177253_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13635
Sequence Type Exemplar sequence
Representative Public ID R186.1 NCBI
Target Description R186.1 /REP_DB=WormBase Gene ID /WP=CE12870 /TR=Q22009 /GB=CAB01439.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073842(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073842 NCBI Caenorhabditis elegans R186.1 (R186.1) mRNA, complete cds. 11/11 A 
SNAP00000011786 ENSEMBL cdna:SNAP chromosome:CEL140:V:12964316:12965910:-1 11/11 None
GENEFINDER00000011793 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12964316:12965910:-1 11/11 None
R186.1 ENSEMBL cdna:known chromosome:CEL140:V:12964190:12965926:-1 gene:R186.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12964315-12965910(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R186.1
Entrez Gene 179780 Entrez gene
SwissProt Q22009 EMBL-EBI
WormBase CE12870 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:252981_AT expressed protein at
CANINE_2:CFA.18005.1.S1_S_AT similar to Ser/Thr-rich protein T10 in DGCR region cfa
CANINE_2:CFAAFFX.21929.1.S1_AT similar to Ser/Thr-rich protein T10 in DGCR region cfa
CANINE_2:CFAAFFX.21930.1.S1_S_AT similar to Ser/Thr-rich protein T10 in DGCR region cfa
DROSOPHILA_2:1634925_AT dm
DROSGENOME1:153477_AT dm
CHICKEN:GGAAFFX.13043.1.S1_S_AT similar to MGC68627 protein gga
CHICKEN:GGAAFFX.1358.2.S1_S_AT similar to MGC68627 protein gga
U133_X3P:HS.308154.0.A1_3P_AT hypothetical protein DKFZp761P1121 hs
HG-U95E:75974_AT hypothetical protein DKFZp761P1121 hs
HG-U95E:77436_AT hypothetical protein DKFZp761P1121 hs
HG-U95E:89857_AT hypothetical protein DKFZp761P1121 hs
HG-U133_PLUS_2:235396_AT Hypothetical protein DKFZp761P1121 hs
HG-U133B:235396_AT Hypothetical protein DKFZp761P1121 hs
HG-U95E:75128_AT Hypothetical protein DKFZp761P1121 hs
MU11KSUBB:X74504_S_AT DNA segment, Chr 16, human D22S680E, expressed mm
MG-U74AV2:95025_AT DNA segment, Chr 16, human D22S680E, expressed mm
MOE430A:1448900_AT DNA segment, Chr 16, human D22S680E, expressed mm
MOUSE430A_2:1448900_AT DNA segment, Chr 16, human D22S680E, expressed mm
MOUSE430_2:1448900_AT DNA segment, Chr 16, human D22S680E, expressed mm
MOUSE430_2:1446626_AT DNA segment, Chr 16, human D22S680E, expressed (D16H22S680E), mRNA mm
MOE430B:1446626_AT DNA segment, Chr 16, human D22S680E, expressed (D16H22S680E), mRNA mm
RG-U34A:RC_AA891032_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
RG-U34A:RC_AA799663_G_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
RG-U34A:RC_AA799663_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
RG-U34B:RC_AI010662_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
RG-U34C:RC_AI103854_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
RAE230A:1386503_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
RAT230_2:1386503_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
RAE230A:1372044_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
RAT230_2:1372044_AT similar to Ser/Thr-rich protein T10 in DGCR region (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB01439 Hypothetical protein R186.1 [Caenorhabditis elegans] ref|NP_506243.1| R186.1 [Caenorhabditis elegans] 1.0E-158
blast CAE64708 Hypothetical protein CBG09494 [Caenorhabditis briggsae] 1.0E-118
blast CAB60443 Hypothetical protein Y80D3A.9 [Caenorhabditis elegans] ref|NP_507692.1| Y80D3A.9 [Caenorhabditis elegans] 2.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR008551 EMBL-EBI Protein of unknown function DUF833 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:177253_AT
gagtcaaagaacttacatgcaccaagtagaggaggaatagtgaacgagtttttgaatgct
aatgatacttcgaaaatgatagagtcattgaagaaatgtgcatcaaaatacaatggtttt
caattagttgcagttgaaaaaaactccaccggtttgtatgaagttcgaacgttggccaat
caacaagtggacgaaattgaagtttgtcaactgaaggatgagtaccatgtcgtctcaaat
agtccacctacaaaaccgtatcaaaaggcagtccaaggcaaaaaacttctgagagaacac
cttgaaaatagtgaccaattcagtgttgatcaaatttttgagaagttactttcgattgca
aaaaatacgacacaatggtatccagatgctcagctacagtatcaaacacagaatgtagaa
gagtacaatcgtccactctctgcgatatttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTCAAAGAACTTACATGCACCAA 615 399 235 Antisense
ACTCCACCGGTTTGTATGAAGTTCG 382 97 377 Antisense
TGAAGTTCGAACGTTGGCCAATCAA 270 567 393 Antisense
TGAAGGATGAGTACCATGTCGTCTC 573 575 446 Antisense
CATGTCGTCTCAAATAGTCCACCTA 309 209 460 Antisense
TAGTCCACCTACAAAACCGTATCAA 272 653 474 Antisense
ACCGTATCAAAAGGCAGTCCAAGGC 495 87 489 Antisense
TAGTGACCAATTCAGTGTTGATCAA 117 653 543 Antisense
AATGGTATCCAGATGCTCAGCTACA 162 163 608 Antisense
GATGCTCAGCTACAGTATCAAACAC 45 413 619 Antisense
ATCGTCCACTCTCTGCGATATTTAT 436 35 662 Antisense
Sequence Source Affymetrix Proprietary Database