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[177248_at]

Description R186.2 /REP_DB=WormBase Gene ID /WP=CE12872 /TR=Q22011 /GB=CAB01441.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 51      4grp_total > 28      6grp_cgc > 83      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 28.4
(0.7)
A 		32
(1.3)
A 		5.2
(-3)
A 		42.5
(3)
A 		37
B 13.4
(-1)
A 		23.1
(0.8)
A 		2.7
(-3)
A 		35.3
(3)
A 		33
C 24.7
(-0.7)
A 		48.5
(2.1)
A 		5.5
(-3)
A 		55.9
(3)
A 		50
avr 22.2
(-0.4) 		34.5
(1.5) 		4.5
(-3) 		44.6
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 42.6
(1.1)
A 		32.6
(-1.1)
A 		50.9
(3)
A 		37.9
(0.1)
A 		41.4
(0.9)
A 		24
(-3)
A 		27
B 59.8
(2)
P 		34.1
(-0.7)
A 		69.6
(3)
A 		52.1
(1.2)
A 		26.4
(-1.5)
A 		12
(-3)
A 		58
C 10.4
(-3)
A 		47.6
(0.7)
A 		52.8
(1.2)
A 		71
(3)
A 		31.1
(-1)
A 		26.1
(-1.4)
A 		61
avr 37.6
(-0.3) 		38.1
(-0.2) 		57.8
(3) 		53.7
(2.3) 		33
(-1) 		20.7
(-3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 10.4
(3)
A 		7
(0.7)
A 		1.4
(-3)
A 		6.2
(0.2)
A 		9
B 4.3
(0.2)
A 		5.7
(1.4)
A 		0.6
(-3)
A 		7.5
(3)
A 		7
C 8.2
(0.1)
A 		14
(3)
A 		1.9
(-3)
A 		9.4
(0.7)
A 		12
avr 7.6
(2) 		8.9
(3) 		1.3
(-3) 		7.7
(2.1) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 7.9
(-1.1)
A 		11.6
(2.2)
A 		11.1
(1.7)
A 		9.6
(0.4)
A 		12.5
(3)
A 		5.8
(-3)
A 		7
B 20.8
(3)
P 		11.3
(-0.5)
A 		16.9
(1.6)
A 		13.8
(0.4)
A 		10.7
(-0.7)
A 		4.5
(-3)
A 		16
C 3.8
(-3)
A 		14.5
(3)
A 		13.4
(2.4)
A 		14.3
(2.9)
A 		10.1
(0.5)
A 		8.7
(-0.3)
A 		11
avr 10.8
(0.6) 		12.5
(2) 		13.8
(3) 		12.6
(2) 		11.1
(0.8) 		6.3
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177248_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13742
Sequence Type Exemplar sequence
Representative Public ID R186.2 NCBI
Target Description R186.2 /REP_DB=WormBase Gene ID /WP=CE12872 /TR=Q22011 /GB=CAB01441.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073843(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073843 NCBI Caenorhabditis elegans Serpentine Receptor, class D (delta) family member (srd-35) (srd-35) mRNA, complete cds. 11/11 None
GENEFINDER00000011799 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12967254:12968591:1 11/11 None
R186.2 ENSEMBL cdna:known chromosome:CEL140:V:12966966:12968591:1 gene:R186.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011787 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12966965-12968591(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srd-35
Ensembl R186.2
Entrez Gene 187918 Entrez gene
SwissProt Q22011 EMBL-EBI
WormBase CE12872 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01441 Hypothetical protein R186.2 [Caenorhabditis elegans] ref|NP_506244.1| Serpentine Receptor, class D (delta) family member (srd-35) [Caenorhabditis elegans] 0.0
blast CAB01441 Hypothetical protein R186.2 [Caenorhabditis elegans] ref|NP_506244.1| Serpentine Receptor, class D (delta) family member (srd-35) [Caenorhabditis elegans] 1.0E-139
blast CAE64707 Hypothetical protein CBG09493 [Caenorhabditis briggsae] 4.0E-95
blast CAE64707 Hypothetical protein CBG09493 [Caenorhabditis briggsae] 2.0E-71
blast CAE61422 Hypothetical protein CBG05290 [Caenorhabditis briggsae] 3.0E-41
blast CAE61422 Hypothetical protein CBG05290 [Caenorhabditis briggsae] 5.0E-32
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506244.1 7 23-45,52-74,107-126,146-165,200-222,246-268,283-305
R186.2 7 23-45,52-74,107-126,146-165,200-222,246-268,283-305
GENEFINDER00000011799 5 10-30,50-69,104-126,150-172,187-209
Sequence
Target Sequence 
>C. ELEGANS:177248_AT
ttggaaactcgtcagatctctcttcctattctacattccatgtttggtttctatgtgttg
cgcttttgtagtcgttttgcgaaacgtattaccaaacgaggaactaaataaagaaagttt
ttctgatacgagaatagaatttactaaaattgggaacatgaaattgggtgaattaccaaa
tagaattaactttgcaatgattgcttatgcaatttactgttctcctattatcaccttttg
gtttcattggaaaactaacaagtccttgaacaatgctttgtcaggctcatcgccgtacct
tcgttatcacgcgaaaaatgttatgatgggaattacaattcaagttgttatgcattttat
tttctacattccctttttcacattgtattcttattcacttctcacaggaacaaaaatctt
cgttcaacagtttttcatggcaatgtccccgaatcttgctgcatctttcgatccactcat
caatctgtacttcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGAAACTCGTCAGATCTCTCTTC 282 707 429 Antisense
TCTTCCTATTCTACATTCCATGTTT 57 619 449 Antisense
TGTTTGGTTTCTATGTGTTGCGCTT 296 559 469 Antisense
TGTTGCGCTTTTGTAGTCGTTTTGC 642 559 484 Antisense
GCTTATGCAATTTACTGTTCTCCTA 298 305 631 Antisense
GTTCTCCTATTATCACCTTTTGGTT 520 425 647 Antisense
AACAATGCTTTGTCAGGCTCATCGC 235 135 697 Antisense
GTATTCTTATTCACTTCTCACAGGA 527 451 813 Antisense
TTTTTCATGGCAATGTCCCCGAATC 461 675 859 Antisense
CGAATCTTGCTGCATCTTTCGATCC 196 253 878 Antisense
ATCCACTCATCAATCTGTACTTCGT 539 39 899 Antisense
Sequence Source Affymetrix Proprietary Database