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[177247_at]

Description BE10.2 /REP_DB=WormBase Gene ID /WP=CE15565 /TR=O17554 /GB=CAB07547.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 09      6grp_high1_10_10 > 90      6grp_highest_nonorm > 72      6grp_total > 82      4grp_total > 57      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1004
1 2 3 4 range
A 93.9
(-3)
P 		813
(1.7)
P 		1010.7
(3)
P 		97.8
(-3)
A 		917
B 93.9
(-2.4)
P 		816.7
(2.5)
P 		884.8
(3)
P 		6.6
(-3)
A 		878
C 74.3
(-2.9)
A 		867.7
(2.8)
P 		894.6
(3)
P 		58.9
(-3)
A 		836
avr 87.4
(-2.8) 		832.5
(2.3) 		930
(3) 		54.4
(-3) 		876

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 440
1 2 3 4 5 6 range
A 177
(-3)
P 		550.7
(3)
P 		336.8
(-0.4)
P 		448.1
(1.4)
P 		532.9
(2.7)
P 		245.2
(-1.9)
P 		374
B 117.9
(-3)
P 		452.7
(1.7)
P 		357.9
(0.4)
P 		546.2
(3)
P 		528.1
(2.7)
P 		264.6
(-0.9)
P 		428
C 171.2
(-3)
P 		542.4
(2.8)
P 		363.8
(-0)
P 		483.9
(1.9)
P 		557.7
(3)
P 		268
(-1.5)
P 		387
avr 155.4
(-3) 		515.3
(2.6) 		352.8
(0.1) 		492.7
(2.3) 		539.6
(3) 		259.3
(-1.4) 		384
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 310
1 2 3 4 range
A 34.2
(-2.5)
P 		176.5
(0.9)
P 		264.2
(3)
P 		14.3
(-3)
A 		250
B 29.9
(-2.2)
P 		201.7
(2.9)
P 		205
(3)
P 		1.4
(-3)
A 		204
C 24.6
(-2.7)
A 		249.5
(1.8)
P 		311.8
(3)
P 		9.9
(-3)
A 		302
avr 29.6
(-2.5) 		209.2
(1.8) 		260.3
(3) 		8.5
(-3) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 32.6
(-3)
P 		195.8
(3)
P 		73.4
(-1.5)
P 		113.7
(-0)
P 		160.8
(1.7)
P 		59.7
(-2)
P 		163
B 41
(-3)
P 		150.7
(0.8)
P 		86.9
(-1.4)
P 		144.7
(0.6)
P 		214.5
(3)
P 		98.7
(-1)
P 		174
C 63.5
(-3)
P 		165.5
(2.2)
P 		92.6
(-1.5)
P 		97.3
(-1.3)
P 		180.5
(3)
P 		89.7
(-1.7)
P 		117
avr 45.7
(-3) 		170.7
(2.4) 		84.3
(-1.3) 		118.6
(0.1) 		185.3
(3) 		82.7
(-1.4) 		140
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177247_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7083
Sequence Type Exemplar sequence
Representative Public ID BE10.2 NCBI
Target Description BE10.2 /REP_DB=WormBase Gene ID /WP=CE15565 /TR=O17554 /GB=CAB07547.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067263(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067263 NCBI Caenorhabditis elegans BE10.2 (BE10.2) mRNA, complete cds. 10/11 None
GENEFINDER00000027561 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12793371:12797992:-1 10/11 None
BE10.2 ENSEMBL cdna:known chromosome:CEL140:III:12793298:12798030:-1 gene:BE10.2 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027553 3/11 Cross Hyb Matching Probes None
SNAP00000027554 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12793656-12797988(-) 78.0 100.0
Public Domain and Genome References
Gene Title
Ensembl BE10.2
Entrez Gene 176695 Entrez gene
SwissProt O17554 EMBL-EBI
WormBase CE30592 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605432_AT similar to BE10.2 cfa
CANINE:1596646_AT similar to BE10.2 cfa
CANINE_2:CFA.14573.1.A1_AT similar to BE10.2 cfa
CANINE_2:CFA.14573.1.A1_S_AT similar to BE10.2 cfa
CANINE_2:CFA.1794.1.S1_AT similar to BE10.2 cfa
CANINE_2:CFA.1794.1.S1_S_AT similar to BE10.2 cfa
CANINE_2:CFAAFFX.4490.1.S1_AT similar to BE10.2 cfa
CHICKEN:GGAAFFX.6820.1.S1_AT similar to RIKEN cDNA A530016O06 gene gga
HU35KSUBA:HUM_ALU_AT FLJ16237 protein hs
HU35KSUBB:HUM_ALU_AT FLJ16237 protein hs
HU35KSUBC:HUM_ALU_AT FLJ16237 protein hs
HU35KSUBD:HUM_ALU_AT FLJ16237 protein hs
HUGENEFL:HUM_ALU_AT FLJ16237 protein hs
HC-G110:HUM_ALU_AT FLJ16237 protein hs
HG-U95D:74001_AT FLJ16237 protein hs
HG-U95B:42127_AT FLJ16237 protein hs
HG-U95C:55139_AT FLJ16237 protein hs
HU35KSUBD:RC_AA399949_AT FLJ16237 protein hs
RAE230B:1381574_AT similar to putative protein, with at least 6 transmembrane domains, of ancient origin (58.5 kD) (3N884) (predicted) rn
RAT230_2:1381574_AT similar to putative protein, with at least 6 transmembrane domains, of ancient origin (58.5 kD) (3N884) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB07547 Hypothetical protein BE10.2 [Caenorhabditis elegans] ref|NP_499664.2| BE10.2 [Caenorhabditis elegans] 0.0
blast CAE69532 Hypothetical protein CBG15741 [Caenorhabditis briggsae] 0.0
blast BAC30271.1 unnamed protein product [Mus musculus] 3.0E-70
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499664.2 5 56-75,366-388,393-415,420-442,452-471
BE10.2 5 56-75,366-388,393-415,420-442,452-471
GENEFINDER00000027561 5 56-75,366-388,393-415,420-442,452-471
Sequence
Target Sequence 
>C. ELEGANS:177247_AT
acctccaattccatgctctttgggacattttaattttcaaaggattcacaaaagatgtga
aaggagagcccatgtttcctggagttgtgaacaaattgaaagcaaccgtattcccgccgg
gctggttcccaggggttcctgtcaccccgttctttcattggatgagcatggttaatccag
ctcacggagtacctgagccagagaaacctgttctcagatacagcccacctgcgaggatcc
tagtgaaagtttacgtggcatcgtcattcttgttgttgttggctatattcttccattttg
aatacgaccggaatcatttgagctacttggattgtacagtcaagattgcatactttgtgg
ttacgatgcaatgttttggagcattttttgatatgaaatggtatgcccggtacattgaaa
ttgctcgttgttgtggagttctcatctattacggagtactcatgttcgatcatattggtg
caggaactcatcgtctttttgtcatttcactgcatatcatggctattgcattgtggacga
ctgatgttttggtggaggtaatagagttgcctacctgtccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCTCCAATTCCATGCTCTTTGGGA 53 89 872 Antisense
AGAGCCCATGTTTCCTGGAGTTGTG 330 69 936 Antisense
ACCCCGTTCTTTCATTGGATGAGCA 685 81 1015 Antisense
GGTTAATCCAGCTCACGGAGTACCT 317 501 1041 Antisense
AAACCTGTTCTCAGATACAGCCCAC 674 125 1075 Antisense
CCCACCTGCGAGGATCCTAGTGAAA 134 259 1095 Antisense
TGGCATCGTCATTCTTGTTGTTGTT 148 547 1127 Antisense
TGCCCGGTACATTGAAATTGCTCGT 374 583 1275 Antisense
ATTGGTGCAGGAACTCATCGTCTTT 162 5 1345 Antisense
CATCGTCTTTTTGTCATTTCACTGC 47 213 1360 Antisense
GGTAATAGAGTTGCCTACCTGTCCG 41 503 1428 Antisense
Sequence Source Affymetrix Proprietary Database