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[177245_at]

Description M153.3 /REP_DB=WormBase Gene ID /WP=CE03513 /TR=Q21546 /GB=CAA91945.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 53      4grp_total > 40      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 61.8
(0.1)
A 		42.3
(-3)
A 		62.7
(0.2)
A 		80.1
(3)
A 		38
B 26.6
(-3)
A 		50.2
(-0.3)
A 		78.3
(3)
A 		40.4
(-1.4)
A 		52
C 59.8
(-3)
A 		123.9
(3)
A 		106.2
(1.3)
A 		80.3
(-1.1)
A 		64
avr 49.4
(-3) 		72.1
(1.1) 		82.4
(3) 		66.9
(0.2) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 29.4
(-1.9)
A 		44
(-0.8)
A 		77.1
(1.8)
A 		39.2
(-1.2)
A 		91.9
(3)
A 		16.1
(-3)
A 		76
B 76.6
(0.5)
A 		58
(-0.6)
A 		120.9
(3)
A 		15.6
(-3)
A 		72.7
(0.3)
A 		42.5
(-1.5)
A 		105
C 54
(-0.8)
A 		86
(3)
A 		57.7
(-0.3)
A 		78.2
(2.1)
A 		44.3
(-1.9)
A 		35.1
(-3)
A 		51
avr 53.3
(-0.5) 		62.7
(0.5) 		85.2
(3) 		44.3
(-1.5) 		69.6
(1.3) 		31.2
(-3) 		54
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 22.5
(3)
A 		9.2
(-3)
A 		16.4
(0.2)
A 		11.7
(-1.9)
A 		13
B 8.5
(-3)
A 		12.4
(-0.6)
A 		18.1
(3)
A 		8.6
(-2.9)
A 		10
C 19.8
(-1.4)
A 		35.6
(2.6)
A 		37
(3)
A 		13.5
(-3)
A 		24
avr 16.9
(-0.3) 		19.1
(0.7) 		23.8
(3) 		11.3
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 5.4
(-2.6)
A 		15.6
(-0.1)
A 		16.8
(0.3)
A 		9.9
(-1.5)
A 		27.7
(3)
A 		3.9
(-3)
A 		24
B 26.7
(2.3)
A 		19.3
(0.6)
A 		29.4
(3)
A 		4.1
(-3)
A 		29.6
(3)
A 		15.8
(-0.2)
A 		26
C 20
(0.4)
A 		26.2
(3)
A 		14.7
(-1.8)
A 		15.7
(-1.3)
A 		14.3
(-1.9)
A 		11.7
(-3)
A 		15
avr 17.4
(0.2) 		20.4
(1.5) 		20.3
(1.5) 		9.9
(-3) 		23.9
(3) 		10.5
(-2.7) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177245_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16076
Sequence Type Exemplar sequence
Representative Public ID M153.3 NCBI
Target Description M153.3 /REP_DB=WormBase Gene ID /WP=CE03513 /TR=Q21546 /GB=CAA91945.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077630(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077630 NCBI Caenorhabditis elegans M153.3 (M153.3) mRNA, complete cds. 11/11 None
GENEFINDER00000043670 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12144035:12148171:1 11/11 None
M153.3 ENSEMBL cdna:known chromosome:CEL140:X:12142840:12145861:1 gene:M153.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043666 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12142839-12145861(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M153.3
Entrez Gene 187484 Entrez gene
SwissProt Q21546 EMBL-EBI
WormBase CE03513 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91945 Hypothetical protein M153.3 [Caenorhabditis elegans] ref|NP_510031.1| M153.3 [Caenorhabditis elegans] 0.0
blast CAA91943 Hypothetical protein M153.1 [Caenorhabditis elegans] ref|NP_510032.1| M153.1 [Caenorhabditis elegans] 1.0E-151
blast CAE69881 Hypothetical protein CBG16221 [Caenorhabditis briggsae] 1.0E-147
blast CAA91945 Hypothetical protein M153.3 [Caenorhabditis elegans] ref|NP_510031.1| M153.3 [Caenorhabditis elegans] 1.0E-115
blast CAE69882 Hypothetical protein CBG16222 [Caenorhabditis briggsae] 1.0E-106
blast CAE69883 Hypothetical protein CBG16223 [Caenorhabditis briggsae] 1.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.038
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.28
Pfam IPR000304 EMBL-EBI Delta 1-pyrroline-5-carboxylate reductase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:177245_AT
ttcaaccaaccccacgatatcagtggccgaacaccatgttgtgtggatagactaacaaca
cttacttgtggaaggatgagactgaacacaccggcaaaatttgctaaacgatgtgcgaat
gatcccgatttcgcacttattcagtgttgctcttcgtgctttgacagaagaagctctttg
agcagtgaaaagtacaatttggtagtgaggagacatttggaggatccaatgaaagctaca
tgcatagatagacgaggaggagactggtgccaaaaaatggcaaacagagagaactattgg
caagacgcaagtttcagcacaattggtggttgtggtgcttttccgtcggcattccgagag
tgtcgaaatacatgtggatattgttcagcaacaatgcaaatgactgttgttaaatatgac
tatcgactggcaacaagcacaccacgatgtgatattaaaacatattttgccaggacggat
gttgaaatgaaaaagcgatacaatccggctaagaaagaattcgacccacttgcccgacta
atatggaaaaggccaaaagacaccgattacttcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAACCAACCCCACGATATCAGTG 628 681 595 Antisense
ATCAGTGGCCGAACACCATGTTGTG 580 27 613 Antisense
AGGATGAGACTGAACACACCGGCAA 353 57 667 Antisense
GAATGATCCCGATTTCGCACTTATT 638 331 711 Antisense
CACTTATTCAGTGTTGCTCTTCGTG 413 197 728 Antisense
GGCAAGACGCAAGTTTCAGCACAAT 466 533 893 Antisense
AATTGGTGGTTGTGGTGCTTTTCCG 294 155 915 Antisense
CTTTTCCGTCGGCATTCCGAGAGTG 708 219 932 Antisense
TCGACTGGCAACAAGCACACCACGA 527 601 1017 Antisense
ACCCACTTGCCCGACTAATATGGAA 263 91 1118 Antisense
GGCCAAAAGACACCGATTACTTCGT 546 539 1145 Antisense
Sequence Source Affymetrix Proprietary Database