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[177243_s_at]

Description M162.4 /REP_DB=WormBase Gene ID /WP=CE26683 /TR=Q9XUC7 /GB=CAB05256.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 11      4grp_total > 12      6grp_cgc > 53      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 30.9
(-1.5)
P 		35.7
(-0.1)
A 		25.5
(-3)
A 		46.5
(3)
A 		21
B 28.8
(-1.1)
A 		8.1
(-3)
A 		17.2
(-2.1)
A 		72.1
(3)
P 		64
C 35.4
(0.2)
P 		17.4
(-1.5)
A 		1.3
(-3)
A 		65.5
(3)
P 		64
avr 31.7
(-0.8) 		20.4
(-2.3) 		14.7
(-3) 		61.4
(3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 30.1
(2.5)
A 		13.7
(-0.8)
A 		32.7
(3)
P 		11.8
(-1.2)
A 		29.1
(2.3)
A 		3
(-3)
A 		30
B 19.3
(-1.1)
A 		41.6
(3)
P 		9.4
(-2.9)
A 		16.3
(-1.7)
A 		28.5
(0.6)
P 		9
(-3)
A 		33
C 6
(-2.5)
A 		45.3
(3)
P 		24.4
(0.1)
A 		15
(-1.2)
A 		2.2
(-3)
A 		22.4
(-0.2)
P 		43
avr 18.5
(-1.1) 		33.5
(3) 		22.2
(-0.1) 		14.4
(-2.2) 		19.9
(-0.7) 		11.5
(-3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 11.3
(3)
P 		7.7
(-1.7)
A 		6.7
(-3)
A 		6.8
(-2.9)
A 		5
B 9.2
(0.2)
A 		2
(-3)
A 		4
(-2.1)
A 		15.4
(3)
P 		13
C 11.7
(3)
P 		5
(-0.6)
A 		0.5
(-3)
A 		11
(2.6)
P 		11
avr 10.7
(2.7) 		4.9
(-2) 		3.7
(-3) 		11.1
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 5.6
(0.6)
A 		4.9
(0.1)
A 		7.1
(1.7)
P 		3
(-1.3)
A 		8.8
(3)
A 		0.7
(-3)
A 		8
B 6.7
(-0.7)
A 		13.9
(3)
P 		2.3
(-3)
A 		4.3
(-2)
A 		11.6
(1.8)
P 		3.4
(-2.4)
A 		12
C 2.2
(-2.3)
A 		13.8
(3)
P 		6.2
(-0.5)
A 		3
(-1.9)
A 		0.7
(-3)
A 		7.5
(0.1)
P 		13
avr 4.8
(-1.9) 		10.9
(3) 		5.2
(-1.6) 		3.4
(-3) 		7
(-0.1) 		3.9
(-2.6) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177243_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14758
Sequence Type Exemplar sequence
Representative Public ID M162.4 NCBI
Target Description M162.4 /REP_DB=WormBase Gene ID /WP=CE26683 /TR=Q9XUC7 /GB=CAB05256.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075433(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075433 NCBI Caenorhabditis elegans Serpentine Receptor, class T family member (srt-45) (srt-45) mRNA, complete cds. 11/11 A 
SNAP00000032748 ENSEMBL cdna:SNAP chromosome:CEL140:V:19771732:19779362:1 11/11 None
SNAP00000032749 ENSEMBL cdna:SNAP chromosome:CEL140:V:19782127:19783940:1 11/11 None
GENEFINDER00000032756 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19778121:19785224:1 11/11 None
M162.4 ENSEMBL cdna:pseudogene chromosome:CEL140:V:19778121:19780312:1 gene:M162.4 11/11 A 
M162.3 ENSEMBL cdna:known chromosome:CEL140:V:19782127:19783959:1 gene:M162.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000003508 2/11 Cross Hyb Matching Probes None
SNAP00000003509 1/11 Cross Hyb Matching Probes None
SNAP00000003510 3/11 Cross Hyb Matching Probes None
GENEFINDER00000003570 2/11 Cross Hyb Matching Probes None
GENEFINDER00000003580 3/11 Cross Hyb Matching Probes None
Y57G11C.28 2/11 Cross Hyb Matching Probes None
Y57G11C.30 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19778120-19779362(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srt-45
Ensembl M162.4
M162.3
Entrez Gene 187487 Entrez gene
SwissProt Q9XUC8 EMBL-EBI
WormBase CE18893 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05255 Hypothetical protein M162.3 [Caenorhabditis elegans] ref|NP_507834.1| Serpentine Receptor, class T family member (srt-45) [Caenorhabditis elegans] 0.0
blast T23774 hypothetical protein M162.4 - Caenorhabditis elegans 1.0E-162
blast CAB05255 Hypothetical protein M162.3 [Caenorhabditis elegans] ref|NP_507834.1| Serpentine Receptor, class T family member (srt-45) [Caenorhabditis elegans] 1.0E-152
blast T23774 hypothetical protein M162.4 - Caenorhabditis elegans 1.0E-145
blast T23774 hypothetical protein M162.4 - Caenorhabditis elegans 1.0E-143
blast CAB05255 Hypothetical protein M162.3 [Caenorhabditis elegans] ref|NP_507834.1| Serpentine Receptor, class T family member (srt-45) [Caenorhabditis elegans] 1.0E-143
blast CAB05255 Hypothetical protein M162.3 [Caenorhabditis elegans] ref|NP_507834.1| Serpentine Receptor, class T family member (srt-45) [Caenorhabditis elegans] 1.0E-140
blast CAE68187 Hypothetical protein CBG13847 [Caenorhabditis briggsae] 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 1.1E-82
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 4.5E-75
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 4.3E-41
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 4.3E-23
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 6.9E-71
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 4.3E-41
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 4.3E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507834.1 7 36-58,71-93,108-130,151-173,201-223,244-266,270-292
M162.3 7 36-58,71-93,108-130,151-173,201-223,244-266,270-292
SNAP00000032748 6 65-87,100-122,137-159,180-202,236-253,274-293
SNAP00000032749 5 36-58,71-93,108-130,151-173,197-219
GENEFINDER00000032756 11 41-63,70-92,97-119,140-162,192-209,230-249,312-334,341-363,368-390,411-433,453-475
Sequence
Target Sequence 
>C. ELEGANS:177243_S_AT
gtatccaacgtttacttatgtggctggaggaattgcattaaatacctggtgcatggcgtg
tgcagctaccatgtccctattttccaatcggataataagtattggcttcaggaagtatgc
agatgtaatcgagaagaaactcgcctatacttcaatcagttttgtacttttctatggatt
ctacatctgttggttttcacctacaattgtgttcaattcagtatttatggcatggattcc
tgatccattatctgaggaagtacccagtaaagaggcagctgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATCCAACGTTTACTTATGTGGCT 512 455 318 Antisense
CGTTTACTTATGTGGCTGGAGGAAT 394 241 326 Antisense
TACTTATGTGGCTGGAGGAATTGCA 617 643 330 Antisense
GGCTTCAGGAAGTATGCAGATGTAA 395 537 421 Antisense
AAGAAACTCGCCTATACTTCAATCA 195 153 451 Antisense
ACTTCAATCAGTTTTGTACTTTTCT 296 103 466 Antisense
GTTTTGTACTTTTCTATGGATTCTA 136 449 476 Antisense
GTATTTATGGCATGGATTCCTGATC 152 453 538 Antisense
GGATTCCTGATCCATTATCTGAGGA 225 509 551 Antisense
TCCATTATCTGAGGAAGTACCCAGT 628 589 561 Antisense
AGTACCCAGTAAAGAGGCAGCTGCA 69 63 576 Antisense
Sequence Source Affymetrix Proprietary Database