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[177227_at]

Description M88.3 /REP_DB=WormBase Gene ID /WP=CE01038 /TR=Q21600 /GB=CAA84333.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 05      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 41.1
(-0.7)
A 		5
(-3)
A 		13
(-2.5)
A 		100.6
(3)
A 		96
B 59.1
(3)
A 		31.8
(-1.4)
A 		34.1
(-1)
A 		22
(-3)
A 		37
C 18.6
(-2.4)
A 		5.9
(-3)
A 		14.1
(-2.6)
A 		125.8
(3)
A 		120
avr 39.6
(-0.8) 		14.2
(-3) 		20.4
(-2.5) 		82.8
(3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 15.7
(-2.7)
A 		12.3
(-3)
A 		54.1
(0.9)
A 		58.4
(1.2)
A 		49.6
(0.4)
A 		77.4
(3)
A 		65
B 9.8
(-2.9)
A 		22.6
(-1.5)
A 		9
(-3)
A 		11.9
(-2.7)
A 		64.2
(3)
A 		61.1
(2.7)
A 		55
C 37.3
(-1.1)
A 		67.2
(1.1)
A 		10.1
(-3)
A 		38.7
(-1)
A 		82.8
(2.2)
A 		94.5
(3)
A 		84
avr 20.9
(-3) 		34
(-1.6) 		24.4
(-2.6) 		36.3
(-1.4) 		65.5
(1.7) 		77.7
(3) 		57
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 15
(3)
A 		1.1
(-3)
A 		3.4
(-2)
A 		14.7
(2.9)
A 		14
B 18.8
(3)
A 		7.9
(-1.6)
A 		7.9
(-1.6)
A 		4.7
(-3)
A 		14
C 6.2
(-1.6)
A 		1.7
(-3)
A 		4.9
(-2)
A 		21.2
(3)
A 		20
avr 13.3
(2.9) 		3.6
(-3) 		5.4
(-1.9) 		13.5
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 2.9
(-3)
A 		4.4
(-2.4)
A 		11.8
(0.3)
A 		14.8
(1.5)
A 		15
(1.5)
A 		18.9
(3)
A 		16
B 3.4
(-2.7)
A 		7.5
(-1.7)
A 		2.2
(-3)
A 		3.2
(-2.7)
A 		26.1
(3)
A 		22.8
(2.2)
A 		24
C 13.8
(-0.7)
A 		20.5
(0.7)
A 		2.6
(-3)
A 		7.8
(-1.9)
A 		26.8
(2)
A 		31.6
(3)
A 		29
avr 6.7
(-2.6) 		10.8
(-1.3) 		5.5
(-3) 		8.6
(-2) 		22.6
(2.4) 		24.4
(3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177227_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6431
Sequence Type Exemplar sequence
Representative Public ID M88.3 NCBI
Target Description M88.3 /REP_DB=WormBase Gene ID /WP=CE01038 /TR=Q21600 /GB=CAA84333.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065520(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065520 NCBI Caenorhabditis elegans M88.3 (M88.3) mRNA, complete cds. 11/11 None
SNAP00000031340 ENSEMBL cdna:SNAP chromosome:CEL140:III:4543588:4544939:-1 11/11 None
GENEFINDER00000031350 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4543588:4544939:-1 11/11 None
M88.3 ENSEMBL cdna:known chromosome:CEL140:III:4543588:4544939:-1 gene:M88.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026086 1/11 Cross Hyb Matching Probes None
GENEFINDER00000026096 3/11 Cross Hyb Matching Probes None
B0285.11 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4543587-4544939(-) 100.0 100.0
chrIII:4350793-4352134(-) 93.15 93.15
Public Domain and Genome References
Gene Title
Ensembl M88.3
Entrez Gene 187471 Entrez gene
SwissProt Q21600 EMBL-EBI
WormBase CE01038 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84333 Hypothetical protein M88.3 [Caenorhabditis elegans] ref|NP_497921.1| M88.3 [Caenorhabditis elegans] 1.0E-171
blast CAE68188 Hypothetical protein CBG13848 [Caenorhabditis briggsae] 5.0E-89
blast CAE74568 Hypothetical protein CBG22332 [Caenorhabditis briggsae] 4.0E-60
Sequence
Target Sequence 
>C. ELEGANS:177227_AT
gtctccgtgcccgtgaaatgaaatctcgtggaattgatgtcaaatcagcttcatctgcat
cgagccgtacgagtccttctccacatgaaagttctgaggagacggattgtgctgtcgggt
cggtgatggacaacctttccaatgcaaagggagacttccggctgatttatccattgcttc
aggaaatgattggtgtaaagctgtatgttttggagcagctggatattctcggagttcaac
acaacgttgtcgaattggaagtacaatcgaccacaaaagtcaacagagtgatcacttcgc
ttcttcgcgaatacttccaaacgttgccacctgatcaatcgggagtactgtaccacgtgg
aattggagcctggatggtcacgtagagttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTCCGTGCCCGTGAAATGAAATC 312 471 560 Antisense
TTCATCTGCATCGAGCCGTACGAGT 592 685 609 Antisense
GAGTCCTTCTCCACATGAAAGTTCT 7 399 630 Antisense
AGGAGACGGATTGTGCTGTCGGGTC 82 57 656 Antisense
GGGTCGGTGATGGACAACCTTTCCA 500 495 676 Antisense
TTCCGGCTGATTTATCCATTGCTTC 366 699 715 Antisense
GCAGCTGGATATTCTCGGAGTTCAA 98 315 774 Antisense
ACAGAGTGATCACTTCGCTTCTTCG 164 109 842 Antisense
CGCTTCTTCGCGAATACTTCCAAAC 464 253 857 Antisense
GTTGCCACCTGATCAATCGGGAGTA 242 439 882 Antisense
GAGCCTGGATGGTCACGTAGAGTTC 268 385 925 Antisense
Sequence Source Affymetrix Proprietary Database