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[177224_at]

Description DY3.6 /REP_DB=WormBase Gene ID /WP=CE15750 /TR=O45323 /GB=CAB09415.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 86      6grp_total > 95      4grp_total > 03      6grp_cgc > 88      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3167
1 2 3 4 range
A 945.4
(-2)
P 		555.7
(-2.9)
P 		491.7
(-3)
P 		3207.8
(3)
P 		2716
B 775.1
(-2.5)
P 		662.2
(-2.7)
P 		500.4
(-3)
P 		3571.7
(3)
P 		3071
C 1060.6
(-1.9)
P 		475.2
(-3)
P 		571
(-2.8)
P 		3641.7
(3)
P 		3167
avr 927
(-2.2) 		564.4
(-2.9) 		521
(-3) 		3473.7
(3) 		2953

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 563
1 2 3 4 5 6 range
A 776.7
(-1.6)
P 		891.7
(-0.4)
P 		646.9
(-3)
P 		994.1
(0.7)
P 		1209.4
(3)
P 		1061.1
(1.4)
P 		563
B 985.5
(0.3)
P 		927.8
(-1)
P 		837.6
(-3)
P 		1048.5
(1.7)
P 		980.7
(0.2)
P 		1104.6
(3)
P 		267
C 857.5
(-0.8)
P 		978.1
(1.7)
P 		749.3
(-3)
P 		1041.4
(3)
P 		1031.7
(2.8)
P 		887.6
(-0.2)
P 		292
avr 873.2
(-0.7) 		932.5
(0.4) 		744.6
(-3) 		1028
(2.2) 		1073.9
(3) 		1017.8
(2) 		329
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 645
1 2 3 4 range
A 344.2
(0.8)
P 		120.6
(-3)
P 		128.5
(-2.9)
P 		469.6
(3)
P 		349
B 247.2
(-1.8)
P 		163.6
(-2.6)
P 		116
(-3)
P 		761.1
(3)
P 		645
C 351.1
(-0.3)
P 		136.6
(-3)
P 		199
(-2.2)
P 		614.1
(3)
P 		478
avr 314.2
(-0.8) 		140.3
(-3) 		147.8
(-2.9) 		614.9
(3) 		475

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 271
1 2 3 4 5 6 range
A 143.2
(-2.9)
P 		317.1
(1.7)
P 		140.9
(-3)
P 		252.1
(-0)
P 		364.9
(3)
P 		258.3
(0.1)
P 		224
B 342.9
(1)
P 		308.8
(0)
P 		203.4
(-3)
P 		277.7
(-0.9)
P 		398.4
(2.6)
P 		411.9
(3)
P 		209
C 317.8
(2.3)
P 		298.5
(1.5)
P 		190.7
(-3)
P 		209.4
(-2.2)
P 		333.8
(3)
P 		297.1
(1.5)
P 		143
avr 268
(-0.1) 		308.1
(1.2) 		178.3
(-3) 		246.4
(-0.8) 		365.7
(3) 		322.4
(1.6) 		187
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177224_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1436
Sequence Type Exemplar sequence
Representative Public ID DY3.6 NCBI
Target Description DY3.6 /REP_DB=WormBase Gene ID /WP=CE15750 /TR=O45323 /GB=CAB09415.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059975(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059975 NCBI Caenorhabditis elegans MAFBx (F-box protein) homolog family member (mfb-1) (mfb-1) mRNA, complete cds. 11/11 None
SNAP00000022839 ENSEMBL cdna:SNAP chromosome:CEL140:I:8788390:8791583:-1 11/11 None
GENEFINDER00000022845 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8788390:8793511:-1 11/11 None
DY3.6 ENSEMBL cdna:known chromosome:CEL140:I:8787862:8793540:-1 gene:DY3.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8788320-8793442(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mfb-1
Ensembl DY3.6
Entrez Gene 183975 Entrez gene
SwissProt O45323 EMBL-EBI
WormBase CE15750 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.3053.1.A1_AT similar to F-box only protein 25 isoform 2 cfa
CANINE_2:CFAAFFX.15956.1.S1_S_AT similar to F-box only protein 25 isoform 2 cfa
DROSOPHILA_2:1640228_AT dm
DROSGENOME1:148597_AT dm
CHICKEN:GGA.10623.1.S1_S_AT similar to F-box only protein 25 isoform 1; F-box protein Fbx25; F-box only protein 25 gga
CHICKEN:GGAAFFX.24941.1.S1_AT similar to F-box only protein 25 isoform 1; F-box protein Fbx25; F-box only protein 25 gga
CHICKEN:GGAAFFX.24941.1.S1_S_AT similar to F-box only protein 25 isoform 1; F-box protein Fbx25; F-box only protein 25 gga
HG-U133_PLUS_2:225591_AT F-box protein 25 hs
HG-U133B:225591_AT F-box protein 25 hs
HU35KSUBC:RC_AA463859_AT F-box protein 25 hs
HU35KSUBD:RC_T98785_AT F-box protein 25 hs
HU35KSUBD:RC_H82839_S_AT F-box protein 25 hs
U133_X3P:HS.81001.0.A1_3P_S_AT F-box protein 25 hs
HG-U95C:65955_AT F-box protein 25 hs
HU35KSUBB:RC_Z38624_AT F-box protein 25 hs
U133_X3P:HS.7063.0.S1_3P_AT F-box protein 25 hs
HG-U133_PLUS_2:232254_AT F-box protein 25 hs
HG-U133B:232254_AT F-box protein 25 hs
HG-U95B:47838_AT F-box protein 25 hs
HG-U95C:60544_AT F-box protein 25 hs
HG-U95D:72796_AT F-box protein 25 hs
HU35KSUBB:RC_T91839_AT F-box protein 25 hs
HU35KSUBC:RC_N30562_AT F-box protein 25 hs
MG-U74BV2:112330_AT F-box only protein 25 mm
MG-U74BV2:165369_F_AT F-box only protein 25 mm
MOE430A:1417535_AT F-box only protein 25 mm
MOUSE430A_2:1417535_AT F-box only protein 25 mm
MOUSE430_2:1417535_AT F-box only protein 25 mm
MU19KSUBA:TC17534_AT F-box only protein 25, mRNA (cDNA clone MGC:25973 IMAGE:4240953) mm
MU19KSUBA:TC21666_AT F-box only protein 25, mRNA (cDNA clone MGC:25973 IMAGE:4240953) mm
MU19KSUBA:TC21666_G_AT F-box only protein 25, mRNA (cDNA clone MGC:25973 IMAGE:4240953) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4190 aspartic-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB09415 Hypothetical protein DY3.6 [Caenorhabditis elegans] ref|NP_492376.1| MAFBx (F-box protein) homolog family member (mfb-1) [Caenorhabditis elegans] 0.0
blast CAE66972 Hypothetical protein CBG12367 [Caenorhabditis briggsae] 0.0
blast CAB09415 Hypothetical protein DY3.6 [Caenorhabditis elegans] ref|NP_492376.1| MAFBx (F-box protein) homolog family member (mfb-1) [Caenorhabditis elegans] 1.0E-142
blast CAE66972 Hypothetical protein CBG12367 [Caenorhabditis briggsae] 1.0E-134
blast NP_001014261 hypothetical protein LOC364637 [Rattus norvegicus] gb|AAH82088.1| Hypothetical LOC364637 [Rattus norvegicus] sp|Q641X7|FBX25_RAT F-box only protein 6.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 8.3E-4
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 8.3E-4
Sequence
Target Sequence 
>C. ELEGANS:177224_AT
atctcttttgggtattctcagtgccatttgcttccgatcatcaaacgaagatgttaacgt
gtcaactgccaaagatctagtcaaacagttcggtaacgggttggataatgtgaacgtgtg
tggatctccacaattagtgtctcgtcatcatcaaactgcctcatcacttcttgatctcat
atctgaaaataatgttcgaacggctgctgatgccgatgatgaaagtgctcttacattctt
tgatcttccgagagagattgttcttctaattcttcgtcgtctccctgatcataattccct
tttggagactgcccaagttcatgaagctcttcaagacatgataaatggcgaggacaaaat
ttggaaatccctttgcacatttcacttccaagaatatcaaatcaaacaacagaaaacctc
aggaaaatcgtggcgtcaagcctttttcgacctcaaaaaatatcacgggtgccgtgaact
ctacgctgatctcattcacatttgttgccattgcaaagctcttttctggaaaagtcttgg
acatccatgtgtccgagagacaactgcaccatctgttagagtcactcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCTCTTTTGGGTATTCTCAGTGCC 376 33 531 Antisense
TGCCATTTGCTTCCGATCATCAAAC 551 585 552 Antisense
GGATCTCCACAATTAGTGTCTCGTC 167 513 652 Antisense
GTGCTCTTACATTCTTTGATCTTCC 576 479 755 Antisense
GAGATTGTTCTTCTAATTCTTCGTC 389 397 784 Antisense
CCCTGATCATAATTCCCTTTTGGAG 631 259 813 Antisense
GACTGCCCAAGTTCATGAAGCTCTT 184 375 837 Antisense
GAAATCCCTTTGCACATTTCACTTC 667 361 894 Antisense
ATCGTGGCGTCAAGCCTTTTTCGAC 224 35 957 Antisense
TGAACTCTACGCTGATCTCATTCAC 698 573 1005 Antisense
CTGCACCATCTGTTAGAGTCACTCC 603 235 1094 Antisense
Sequence Source Affymetrix Proprietary Database