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[177223_s_at]

Description Y53G8AM.C /REP_DB=WormBase Gene ID /WP=CE25412 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59532.1
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 04      4grp_highest_8_8 > 04      4grp_high_8_8 > 38      6grp_highest_nonorm > 81      6grp_total > 24      4grp_total > 33      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 165
1 2 3 4 range
A 211.3
(-3)
P 		373.8
(3)
P 		307.2
(0.5)
P 		371.3
(2.9)
P 		163
B 247.4
(-3)
P 		323.5
(0.6)
P 		372.6
(3)
P 		341
(1.5)
P 		125
C 249.6
(-3)
P 		376.4
(3)
P 		318.8
(0.3)
P 		300.5
(-0.6)
P 		127
avr 236.1
(-3) 		357.9
(3) 		332.9
(1.8) 		337.6
(2) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 174
1 2 3 4 5 6 range
A 309.4
(2.6)
P 		264.7
(0.1)
P 		316
(3)
P 		230.7
(-1.8)
P 		228.6
(-2)
P 		210.1
(-3)
P 		106
B 358.9
(3)
P 		327.3
(1.9)
P 		184.7
(-3)
P 		210
(-2.1)
P 		247.1
(-0.9)
P 		196.9
(-2.6)
P 		174
C 249.5
(-0.6)
P 		323.4
(3)
P 		302.7
(2)
P 		227.8
(-1.7)
P 		200.7
(-3)
P 		200.8
(-3)
P 		123
avr 305.9
(3) 		305.1
(3) 		267.8
(0.8) 		222.8
(-1.8) 		225.5
(-1.7) 		202.6
(-3) 		103
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 76.9
(2.1)
P 		81.1
(3)
P 		80.3
(2.8)
P 		54.3
(-3)
P 		27
B 78.9
(-0.3)
P 		79.9
(0.2)
P 		86.3
(3)
P 		72.7
(-3)
P 		14
C 82.6
(0.2)
P 		108.2
(2.7)
P 		111.1
(3)
P 		50.7
(-3)
P 		60
avr 79.5
(0.6) 		89.7
(2.5) 		92.6
(3) 		59.2
(-3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 57
(-2.2)
P 		94.2
(3)
P 		68.8
(-0.5)
P 		58.5
(-2)
P 		69
(-0.5)
P 		51.1
(-3)
P 		43
B 124.9
(3)
P 		109
(1.8)
P 		44.9
(-3)
P 		55.6
(-2.2)
P 		100.4
(1.2)
P 		73.4
(-0.9)
P 		80
C 92.5
(2.3)
P 		98.7
(3)
P 		77
(0.5)
P 		45.8
(-3)
P 		64.9
(-0.8)
P 		67.2
(-0.6)
P 		53
avr 91.5
(1.8) 		100.6
(3) 		63.6
(-1.7) 		53.3
(-3) 		78.1
(0.1) 		63.9
(-1.7) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177223_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18936
Sequence Type Exemplar sequence
Representative Public ID Y53G8AM.C NCBI
Target Description Y53G8AM.C /REP_DB=WormBase Gene ID /WP=CE25412 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59532.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065281(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065281 NCBI Caenorhabditis elegans Y53G8AM.8 (Y53G8AM.8) mRNA, complete cds. 11/11 A 
GENEFINDER00000032330 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3267241:3275248:-1 11/11 None
SNAP00000032323 ENSEMBL cdna:SNAP chromosome:CEL140:III:3267296:3271545:-1 11/11 None
Y53G8AM.8.2 ENSEMBL cdna:known chromosome:CEL140:III:3267167:3278687:-1 gene:Y53G8AM.8 11/11 A 
Y53G8AM.8.1 ENSEMBL cdna:known chromosome:CEL140:III:3267185:3278687:-1 gene:Y53G8AM.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3267240-3269240(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8AM.8
Entrez Gene 175430 Entrez gene
SwissProt Q9BL70 EMBL-EBI
WormBase CE27282 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29931 Hypothetical protein Y53G8AM.8 [Caenorhabditis elegans] ref|NP_497682.1| Y53G8AM.8 [Caenorhabditis elegans] 0.0
blast AAK29931 Hypothetical protein Y53G8AM.8 [Caenorhabditis elegans] ref|NP_497682.1| Y53G8AM.8 [Caenorhabditis elegans] 1.0E-151
blast CAE69478 Hypothetical protein CBG15679 [Caenorhabditis briggsae] 1.0E-147
blast CAE69478 Hypothetical protein CBG15679 [Caenorhabditis briggsae] 1.0E-129
blast CAE69478 Hypothetical protein CBG15679 [Caenorhabditis briggsae] 5.0E-83
Protein Domains
Database ID Description E-Value
scop a.4.1.Centromere-binding All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Centromere-binding 2.00000002337219E-7
Sequence
Target Sequence 
>C. ELEGANS:177223_S_AT
tcccaagaacccgacggtggcggtgatactatattcaatgtgatgagaagttgtcagatt
aaagttgctgataaggaagtcagtcatgctttggacacattgaggacttatattctcgag
cacgacccggcagcgatgagcttgctggtgccgcttcaggagagattggccaccacttcc
agtggggctattg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCAAGAACCCGACGGTGGCGGTG 387 599 556 Antisense
CGACGGTGGCGGTGATACTATATTC 288 251 567 Antisense
GAGAAGTTGTCAGATTAAAGTTGCT 624 389 600 Antisense
GGAAGTCAGTCATGCTTTGGACACA 35 533 630 Antisense
TGGACACATTGAGGACTTATATTCT 37 549 647 Antisense
GGACTTATATTCTCGAGCACGACCC 506 521 659 Antisense
ACCCGGCAGCGATGAGCTTGCTGGT 549 89 680 Antisense
GCAGCGATGAGCTTGCTGGTGCCGC 184 313 685 Antisense
TTGCTGGTGCCGCTTCAGGAGAGAT 186 697 697 Antisense
CTTCAGGAGAGATTGGCCACCACTT 649 221 709 Antisense
GCCACCACTTCCAGTGGGGCTATTG 442 279 724 Antisense
Sequence Source Affymetrix Proprietary Database