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[177222_at]

Description Y53G8AM.C /REP_DB=WormBase Gene ID /WP=CE25412 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59532.1
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 05      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 80      6grp_total > 05      4grp_total > 17      6grp_highest_sub > De      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 466
1 2 3 4 range
A 248.3
(-3)
P 		434.3
(-0.6)
P 		342.3
(-1.8)
P 		714.3
(3)
P 		466
B 341.2
(-2.7)
P 		327.3
(-3)
P 		371.6
(-2)
P 		596.7
(3)
P 		269
C 268.1
(-3)
P 		341.6
(-1.8)
P 		333.8
(-1.9)
P 		638.3
(3)
P 		370
avr 285.9
(-3) 		367.7
(-1.7) 		349.2
(-2) 		649.8
(3) 		364

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 262
1 2 3 4 5 6 range
A 382.9
(3)
P 		278.2
(0.5)
P 		239.5
(-0.4)
P 		182.2
(-1.7)
P 		207.8
(-1.1)
P 		128.4
(-3)
P 		255
B 267.4
(2)
P 		285.1
(3)
P 		272.3
(2.3)
P 		192.1
(-2)
P 		227.9
(-0.1)
P 		173.4
(-3)
P 		112
C 390.1
(3)
P 		276
(0.4)
P 		195.4
(-1.5)
P 		220.6
(-0.9)
P 		239.8
(-0.4)
P 		128.5
(-3)
P 		262
avr 346.8
(3) 		279.8
(1) 		235.7
(-0.3) 		198.3
(-1.4) 		225.2
(-0.6) 		143.4
(-3) 		203
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 90.4
(-2.6)
P 		94.3
(-1.1)
P 		89.5
(-3)
P 		104.6
(3)
P 		15
B 108.8
(0.6)
P 		80.8
(-3)
P 		86.1
(-2.3)
P 		127.2
(3)
P 		46
C 88.7
(-3)
P 		98.2
(-0.9)
P 		116.3
(3)
P 		107.6
(1.1)
P 		28
avr 96
(-1.7) 		91.1
(-3) 		97.3
(-1.3) 		113.1
(3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 70.6
(0.5)
P 		98.9
(3)
P 		52.2
(-1.1)
P 		46.2
(-1.7)
P 		62.7
(-0.2)
P 		31.3
(-3)
P 		68
B 93
(2.7)
P 		94.9
(3)
P 		66.1
(-0.9)
P 		50.9
(-3)
P 		92.6
(2.7)
P 		64.6
(-1.1)
P 		44
C 144.6
(3)
P 		84.2
(-0.6)
P 		49.7
(-2.6)
P 		44.4
(-2.9)
P 		77.6
(-1)
P 		43
(-3)
P 		102
avr 102.7
(3) 		92.7
(1.9) 		56
(-2) 		47.2
(-2.9) 		77.6
(0.3) 		46.3
(-3) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177222_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18936
Sequence Type Exemplar sequence
Representative Public ID Y53G8AM.C NCBI
Target Description Y53G8AM.C /REP_DB=WormBase Gene ID /WP=CE25412 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59532.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065281(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065281 NCBI Caenorhabditis elegans Y53G8AM.8 (Y53G8AM.8) mRNA, complete cds. 9/11 A 
GENEFINDER00000032330 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3267241:3275248:-1 11/11 None
SNAP00000032323 ENSEMBL cdna:SNAP chromosome:CEL140:III:3267296:3271545:-1 9/11 None
Y53G8AM.8.2 ENSEMBL cdna:known chromosome:CEL140:III:3267167:3278687:-1 gene:Y53G8AM.8 9/11 A 
Y53G8AM.8.1 ENSEMBL cdna:known chromosome:CEL140:III:3267185:3278687:-1 gene:Y53G8AM.8 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3267240-3269240(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8AM.8
Entrez Gene 175430 Entrez gene
SwissProt Q9BL70 EMBL-EBI
WormBase CE27282 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29931 Hypothetical protein Y53G8AM.8 [Caenorhabditis elegans] ref|NP_497682.1| Y53G8AM.8 [Caenorhabditis elegans] 0.0
blast AAK29931 Hypothetical protein Y53G8AM.8 [Caenorhabditis elegans] ref|NP_497682.1| Y53G8AM.8 [Caenorhabditis elegans] 1.0E-151
blast CAE69478 Hypothetical protein CBG15679 [Caenorhabditis briggsae] 1.0E-147
blast CAE69478 Hypothetical protein CBG15679 [Caenorhabditis briggsae] 1.0E-129
blast CAE69478 Hypothetical protein CBG15679 [Caenorhabditis briggsae] 5.0E-83
Protein Domains
Database ID Description E-Value
scop a.4.1.Centromere-binding All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Centromere-binding 2.00000002337219E-7
Sequence
Target Sequence 
>C. ELEGANS:177222_AT
gccgagaactcgacatggaatcccatatgcttcaccagcaacccccatccttcttccctc
aaagtgctccagatgagtttgcggcgagcttcttcagttccctaagcggattcccccaac
aaatggttgtccaggaacctccaacgacttcctcgtcgaatcatctccagaatatgatca
attccatgatgacaccgccagtggtggattcccgaatgatgccgagcacctcctcggctg
gagcatcaggagcatcggcttcatcccaagaacccgacggtggcggtgatactatattca
atgtgatgagaagttgtcagattaaagttgctgataaggaagtcagtcatgctttggaca
cattgaggacttatattctcgagcacgacccggcagcgatgagcttgctggtgccgcttc
aggagagattggccaccacttccagtggggctattgggagcaaatatctcggttcatttt
accattatctaaatatttccaactacaaatttatgcaaaaatttatgccaaatatcttcg
cgacatgccatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGAGAACTCGACATGGAATCCCA 192 285 293 Antisense
CATGGAATCCCATATGCTTCACCAG 382 209 306 Antisense
TTCTTCAGTTCCCTAAGCGGATTCC 419 673 382 Antisense
GATTCCCCCAACAAATGGTTGTCCA 326 433 401 Antisense
TTCCTCGTCGAATCATCTCCAGAAT 67 701 441 Antisense
TCAATTCCATGATGACACCGCCAGT 197 633 470 Antisense
GGTGGATTCCCGAATGATGCCGAGC 167 499 495 Antisense
ACCTCCTCGGCTGGAGCATCAGGAG 255 87 520 Antisense
GAGCATCAGGAGCATCGGCTTCATC 158 389 533 Antisense
ATCTCGGTTCATTTTACCATTATCT 358 33 758 Antisense
CAAATATCTTCGCGACATGCCATCC 585 193 821 Antisense
Sequence Source Affymetrix Proprietary Database