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[177218_at]

Description Y53G8AM.F /REP_DB=WormBase Gene ID /WP=CE25415 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59535.1
Cluster 6grp_total > 18      4grp_total > 45      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 244
1 2 3 4 range
A 191
(3)
P 		123.2
(0.5)
A 		40.7
(-2.6)
A 		29.8
(-3)
A 		161
B 248.7
(3)
P 		113
(-0.3)
A 		43.7
(-2)
A 		5
(-3)
A 		244
C 222.7
(3)
P 		108.1
(-0.6)
A 		56
(-2.2)
A 		30.4
(-3)
A 		192
avr 220.8
(3) 		114.8
(-0.2) 		46.8
(-2.2) 		21.7
(-3) 		199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 456
1 2 3 4 5 6 range
A 457.8
(3)
P 		58.8
(-2.3)
A 		36.4
(-2.6)
A 		8.7
(-3)
A 		8.7
(-3)
A 		38.9
(-2.6)
A 		449
B 319.2
(3)
P 		79.3
(-1.5)
A 		23.8
(-2.6)
A 		50.5
(-2.1)
A 		2
(-3)
A 		6.3
(-2.9)
A 		317
C 232.4
(3)
P 		81.5
(-1)
P 		76.7
(-1.1)
A 		39.1
(-2.1)
A 		5.7
(-3)
A 		4.5
(-3)
A 		228
avr 336.5
(3) 		73.2
(-1.8) 		45.6
(-2.3) 		32.8
(-2.5) 		5.5
(-3) 		16.6
(-2.8) 		331
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 69.5
(3)
P 		26.7
(-0.9)
A 		10.6
(-2.4)
A 		4.4
(-3)
A 		65
B 79.3
(3)
P 		27.9
(-0.9)
A 		10.1
(-2.3)
A 		1.1
(-3)
A 		78
C 73.7
(3)
P 		31.1
(-0.7)
A 		19.5
(-1.7)
A 		5.1
(-3)
A 		69
avr 74.2
(3) 		28.6
(-0.9) 		13.4
(-2.2) 		3.5
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 110
1 2 3 4 5 6 range
A 84.4
(3)
P 		20.9
(-1.6)
A 		7.9
(-2.6)
A 		2.2
(-3)
A 		2.6
(-3)
A 		9.5
(-2.5)
A 		82
B 111.1
(3)
P 		26.4
(-1.6)
A 		5.8
(-2.7)
A 		13.4
(-2.3)
A 		0.8
(-3)
A 		2.3
(-2.9)
A 		110
C 86.2
(3)
P 		24.9
(-1.3)
P 		19.5
(-1.7)
A 		7.9
(-2.5)
A 		1.8
(-3)
A 		1.5
(-3)
A 		85
avr 93.9
(3) 		24.1
(-1.5) 		11.1
(-2.4) 		7.8
(-2.6) 		1.7
(-3) 		4.4
(-2.8) 		92
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177218_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18925
Sequence Type Exemplar sequence
Representative Public ID Y53G8AM.F NCBI
Target Description Y53G8AM.F /REP_DB=WormBase Gene ID /WP=CE25415 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59535.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065277(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065277 NCBI Caenorhabditis elegans Y53G8AM.5 (Y53G8AM.5) mRNA, complete cds. 11/11 None
SNAP00000032321 ENSEMBL cdna:SNAP chromosome:CEL140:III:3256719:3258384:-1 11/11 None
GENEFINDER00000032327 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3256719:3258384:-1 11/11 None
Y53G8AM.5 ENSEMBL cdna:known chromosome:CEL140:III:3256719:3258384:-1 gene:Y53G8AM.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3256718-3258384(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8AM.5
Entrez Gene 190237 Entrez gene
SwissProt Q9N3H0 EMBL-EBI
WormBase CE25415 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29926 Hypothetical protein Y53G8AM.5 [Caenorhabditis elegans] ref|NP_497678.1| Y53G8AM.5 [Caenorhabditis elegans] 1.0E-135
blast CAA92485 Hypothetical protein T20D3.2 [Caenorhabditis elegans] ref|NP_501640.1| T20D3.2 [Caenorhabditis elegans] 1.0E-81
blast CAE74046 Hypothetical protein CBG21698 [Caenorhabditis briggsae] 2.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR009673 EMBL-EBI Protein of unknown function DUF1261 1.6E-60
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497678.1 1 5-27
Y53G8AM.5 1 5-27
SNAP00000032321 1 5-27
GENEFINDER00000032327 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:177218_AT
taccgtacacccttccaagtgatgttatccaaacaattgcaaccaaggaaactcgaaaga
gcaatacgtgccaaaatattcagctgaactattgccagaaccaattcaacactttcctcg
gaatcgacgacgcaattacttggcgtgatggatcatcatttttcgaagctgtggagtcgt
atttggatatgaatgtgactgagctcatgaaagtttgcaacgtccgcactcaattctacc
agtgcctgggaagcagctactactcgtgtatcaatcttgaatatctcgccggacaatcaa
attctgacttgggaaatgccttcgactacgtcaaaaccttccgcggactcgagtggatgt
gcagcggcggttatcgtgaagtaatcaatcactggagctgtcttaaggatttcccatcct
ccgattcctataaagcctgcactcaaaccttcaattcagcagtttctacctccaactact
gcccagccgtcgaacaagccggagcctgcttgagtaatgcttatcacgctacttgcggag
ccaacgctggacactacggatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACCGTACACCCTTCCAAGTGATGT 353 649 74 Antisense
CAGAACCAATTCAACACTTTCCTCG 394 205 169 Antisense
TGAAAGTTTGCAACGTCCGCACTCA 170 573 281 Antisense
GGAAGCAGCTACTACTCGTGTATCA 472 531 322 Antisense
ATCAATCTTGAATATCTCGCCGGAC 185 27 343 Antisense
TTGGGAAATGCCTTCGACTACGTCA 229 709 382 Antisense
TCCGATTCCTATAAAGCCTGCACTC 376 595 493 Antisense
GCCTGCACTCAAACCTTCAATTCAG 127 283 508 Antisense
AGCAGTTTCTACCTCCAACTACTGC 314 77 531 Antisense
GCTTATCACGCTACTTGCGGAGCCA 575 303 592 Antisense
AGCCAACGCTGGACACTACGGATGC 20 83 612 Antisense
Sequence Source Affymetrix Proprietary Database