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[177215_s_at]

Description Y45G5AM.B /REP_DB=WormBase Gene ID /WP=CE26399 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23359.1
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      6grp_highest_nonorm > 93      6grp_total > 77      4grp_total > 04      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 313
1 2 3 4 range
A 273.1
(-1.2)
P 		183.8
(-3)
M 		217.1
(-2.3)
M 		473.7
(3)
P 		290
B 247.2
(-1.7)
P 		177.1
(-3)
M 		197.3
(-2.6)
A 		490.1
(3)
P 		313
C 344.6
(0.3)
P 		192.9
(-3)
A 		204.1
(-2.8)
P 		467.1
(3)
P 		274
avr 288.3
(-0.9) 		184.6
(-3) 		206.2
(-2.6) 		477
(3) 		292

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 694
1 2 3 4 5 6 range
A 144.8
(-3)
P 		218.8
(-2.2)
P 		285.1
(-1.5)
P 		274.6
(-1.6)
P 		375.2
(-0.5)
P 		690.8
(3)
P 		546
B 266.9
(-2.7)
P 		239.8
(-3)
P 		266.5
(-2.7)
P 		269.9
(-2.6)
P 		403.2
(-1)
P 		734.1
(3)
P 		494
C 286
(-2.7)
P 		255.8
(-3)
P 		319.9
(-2.3)
P 		319.9
(-2.3)
P 		400
(-1.5)
P 		838.6
(3)
P 		583
avr 232.6
(-3) 		238.1
(-2.9) 		290.5
(-2.3) 		288.1
(-2.4) 		392.8
(-1.2) 		754.5
(3) 		522
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 99.4
(3)
P 		39.9
(-3)
M 		56.7
(-1.3)
M 		69.3
(-0)
P 		60
B 78.8
(0.5)
P 		43.7
(-3)
M 		45.7
(-2.8)
A 		104.4
(3)
P 		61
C 114.1
(3)
P 		55.5
(-3)
A 		71.2
(-1.4)
P 		78.8
(-0.6)
P 		59
avr 97.4
(3) 		46.4
(-3) 		57.9
(-1.6) 		84.2
(1.4) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 26.7
(-3)
P 		77.8
(-0.8)
P 		62.1
(-1.5)
P 		69.6
(-1.2)
P 		113.2
(0.7)
P 		168.2
(3)
P 		142
B 92.9
(-2.2)
P 		79.8
(-2.6)
P 		64.7
(-3)
P 		71.5
(-2.8)
P 		163.8
(-0.2)
P 		273.7
(3)
P 		209
C 106
(-1.8)
P 		78.1
(-2.6)
P 		81.4
(-2.5)
P 		64.3
(-3)
P 		129.4
(-1.2)
P 		280.7
(3)
P 		216
avr 75.2
(-2.8) 		78.6
(-2.6) 		69.4
(-3) 		68.5
(-3) 		135.5
(-0.7) 		240.9
(3) 		172
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177215_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19533
Sequence Type Exemplar sequence
Representative Public ID Y45G5AM.B NCBI
Target Description Y45G5AM.B /REP_DB=WormBase Gene ID /WP=CE26399 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23359.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071759(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071759 NCBI Caenorhabditis elegans Y45G5AM.7 (Y45G5AM.7) mRNA, complete cds. 11/11 A 
SNAP00000024444 ENSEMBL cdna:SNAP chromosome:CEL140:V:4166667:4169564:-1 11/11 None
GENEFINDER00000024463 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4166667:4169564:-1 11/11 None
Y45G5AM.7 ENSEMBL cdna:known chromosome:CEL140:V:4166653:4169649:-1 gene:Y45G5AM.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4166666-4175439(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y45G5AM.7
Entrez Gene 178817 Entrez gene
SwissProt Q95XJ5 EMBL-EBI
WormBase CE27408 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504160 Y45G5AM.7 [Caenorhabditis elegans] gb|AAK84606.1| Hypothetical protein Y45G5AM.7 [Caenorhabditis elegans] 0.0
blast CAE58329 Hypothetical protein CBG01442 [Caenorhabditis briggsae] 8.0E-86
blast CAE58329 Hypothetical protein CBG01442 [Caenorhabditis briggsae] 7.0E-76
blast XP_221489 PREDICTED: similar to KIAA1000 protein [Rattus norvegicus] 8.0E-16
blast XP_221489 PREDICTED: similar to KIAA1000 protein [Rattus norvegicus] 9.0E-16
Sequence
Target Sequence 
>C. ELEGANS:177215_S_AT
gatctgaaaagaggtctcgctgcggattatcgtcaaaagctcggcatggtatcatcactc
gacacacatccgatccgcacgccgctttatgaaaatccattttatgaatggaatcaggaa
aataggaaaagaaatggtaataatgccaaaaaaatcgataattttgaattaaaaatcgag
aatttcgaacgtgctcagtgtccaccatctcctcagcgaacgctcgggattcaaacgtct
gcagatcttgctcccgcagcagctccagtcacatctacaaaacctcgtcaactcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTGAAAAGAGGTCTCGCTGCGG 619 417 2779 Antisense
GAGGTCTCGCTGCGGATTATCGTCA 336 403 2789 Antisense
ATTATCGTCAAAAGCTCGGCATGGT 660 13 2804 Antisense
GCTCGGCATGGTATCATCACTCGAC 706 299 2817 Antisense
TATCATCACTCGACACACATCCGAT 52 661 2828 Antisense
ATCCGCACGCCGCTTTATGAAAATC 247 39 2851 Antisense
ATCTCCTCAGCGAACGCTCGGGATT 266 33 2985 Antisense
GAACGCTCGGGATTCAAACGTCTGC 656 345 2996 Antisense
TCAAACGTCTGCAGATCTTGCTCCC 607 629 3009 Antisense
AGCTCCAGTCACATCTACAAAACCT 119 81 3039 Antisense
ATCTACAAAACCTCGTCAACTCGGA 375 7 3051 Antisense
Sequence Source Affymetrix Proprietary Database