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[177214_at]

Description Y45G5AM.B /REP_DB=WormBase Gene ID /WP=CE26399 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23359.1
Cluster 6grp_total > 87      4grp_total > 09      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 42.9
(-2.7)
A 		38.7
(-3)
A 		42
(-2.8)
A 		123.4
(3)
P 		85
B 29.6
(-3)
A 		35.1
(-1.7)
A 		45.8
(0.8)
A 		55.4
(3)
P 		26
C 26.2
(-3)
A 		54.5
(-0.7)
A 		50.1
(-1.1)
A 		100.3
(3)
P 		74
avr 32.9
(-3) 		42.8
(-2) 		46
(-1.7) 		93
(3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 57.2
(1.1)
A 		19.9
(-3)
A 		58.2
(1.2)
A 		45.5
(-0.2)
A 		71.1
(2.6)
A 		74.3
(3)
M 		54
B 36.8
(-3)
A 		55.6
(-2)
A 		67.4
(-1.4)
A 		99.5
(0.3)
A 		95.5
(0.1)
P 		151.6
(3)
P 		115
C 73.9
(-0.6)
A 		34.2
(-2.5)
A 		53.7
(-1.6)
A 		22.6
(-3)
A 		81.5
(-0.3)
P 		151.9
(3)
P 		129
avr 56
(-1.7) 		36.6
(-3) 		59.8
(-1.4) 		55.9
(-1.7) 		82.7
(0.1) 		125.9
(3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 15.6
(1.5)
A 		8.4
(-3)
A 		11
(-1.4)
A 		18.1
(3)
P 		10
B 9.4
(-1.6)
A 		8.7
(-3)
A 		10.6
(0.7)
A 		11.8
(3)
P 		3
C 8.7
(-3)
A 		15.7
(1.8)
A 		17.4
(3)
A 		16.9
(2.7)
P 		9
avr 11.2
(-2.6) 		10.9
(-3) 		13
(-0.3) 		15.6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 10.5
(-1.6)
A 		7.1
(-3)
A 		12.7
(-0.7)
A 		11.5
(-1.2)
A 		21.4
(3)
A 		18.1
(1.6)
M 		14
B 12.8
(-3)
A 		18.5
(-2.2)
A 		16.4
(-2.5)
A 		26.3
(-1.1)
A 		38.8
(0.6)
P 		56.5
(3)
P 		44
C 27.4
(-0)
A 		10.4
(-2.2)
A 		13.7
(-1.8)
A 		4.6
(-3)
A 		26.4
(-0.2)
P 		50.9
(3)
P 		46
avr 16.9
(-2) 		12
(-3) 		14.3
(-2.5) 		14.1
(-2.6) 		28.9
(0.4) 		41.8
(3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177214_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19533
Sequence Type Exemplar sequence
Representative Public ID Y45G5AM.B NCBI
Target Description Y45G5AM.B /REP_DB=WormBase Gene ID /WP=CE26399 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23359.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071759(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071759 NCBI Caenorhabditis elegans Y45G5AM.7 (Y45G5AM.7) mRNA, complete cds. 10/11 A 
SNAP00000024444 ENSEMBL cdna:SNAP chromosome:CEL140:V:4166667:4169564:-1 10/11 None
GENEFINDER00000024463 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4166667:4169564:-1 10/11 None
Y45G5AM.7 ENSEMBL cdna:known chromosome:CEL140:V:4166653:4169649:-1 gene:Y45G5AM.7 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4166666-4175439(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y45G5AM.7
Entrez Gene 178817 Entrez gene
SwissProt Q95XJ5 EMBL-EBI
WormBase CE27408 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504160 Y45G5AM.7 [Caenorhabditis elegans] gb|AAK84606.1| Hypothetical protein Y45G5AM.7 [Caenorhabditis elegans] 0.0
blast CAE58329 Hypothetical protein CBG01442 [Caenorhabditis briggsae] 8.0E-86
blast CAE58329 Hypothetical protein CBG01442 [Caenorhabditis briggsae] 7.0E-76
blast XP_221489 PREDICTED: similar to KIAA1000 protein [Rattus norvegicus] 8.0E-16
blast XP_221489 PREDICTED: similar to KIAA1000 protein [Rattus norvegicus] 9.0E-16
Sequence
Target Sequence 
>C. ELEGANS:177214_AT
gaaaaaatcgagctaatccagaaagaagctgaggattacagaaaacgtatcgaagaagac
atcagaaatcgacaacaacgccacaaaaaagaagtcgatgctcatcaggagaaattcagc
gctactgtaaatcatcaaaatgctgtaattgaagaactcacgcaaaccgtcaagcagaaa
caatcagaaatcgccgaattgaaggatcagctggaaaagcaggaatctcggattaaagga
gatctgaaaagaggtctcgctgcggattatcgtcaaaagctcggcatggtatcatcactc
gacacacatccgatccgcacgccgctttatgaaaatccattttatgaatggaatcaggaa
aataggaaaagaaatggtaataatgccaaaaaaatcgataattttgaattaaaaatcgag
aatttcgaacgtgctcagtgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAAAATCGAGCTAATCCAGAAAG 155 355 2539 Antisense
CAGAAATCGACAACAACGCCACAAA 379 205 2601 Antisense
CAAAAAAGAAGTCGATGCTCATCAG 339 191 2622 Antisense
TCATCAGGAGAAATTCAGCGCTACT 368 621 2640 Antisense
TCAGCGCTACTGTAAATCATCAAAA 525 625 2654 Antisense
AATGCTGTAATTGAAGAACTCACGC 271 167 2677 Antisense
GAACTCACGCAAACCGTCAAGCAGA 429 349 2692 Antisense
GCAAACCGTCAAGCAGAAACAATCA 576 317 2700 Antisense
ATCGCCGAATTGAAGGATCAGCTGG 490 37 2728 Antisense
CTGGAAAAGCAGGAATCTCGGATTA 545 241 2749 Antisense
CGAGAATTTCGAACGTGCTCAGTGT 685 249 2955 Antisense
Sequence Source Affymetrix Proprietary Database