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[177212_at]

Description Y53G8AM.A /REP_DB=WormBase Gene ID /WP=CE22426 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59530.1
Cluster 6grp_total > 23      4grp_total > 26      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 6.9
(-3)
A 		16.9
(-1.7)
A 		48.3
(2.5)
A 		52.4
(3)
A 		46
B 37.5
(-0.6)
A 		85.1
(3)
A 		19.4
(-2)
A 		5.6
(-3)
A 		80
C 44.9
(0.1)
A 		10.6
(-3)
A 		29.4
(-1.3)
A 		77.9
(3)
A 		67
avr 29.8
(-3) 		37.5
(-0) 		32.4
(-2) 		45.3
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 51.7
(2.1)
A 		30.8
(-0.6)
A 		32.3
(-0.4)
A 		46
(1.3)
A 		59
(3)
A 		12.5
(-3)
A 		47
B 67.2
(3)
A 		46.5
(-0.3)
A 		38.3
(-1.5)
A 		43.3
(-0.8)
A 		34.6
(-2.1)
A 		29
(-3)
A 		38
C 66.5
(2.1)
A 		55.8
(1.3)
A 		77
(3)
A 		44.8
(0.4)
A 		3.6
(-3)
A 		40.5
(0)
A 		73
avr 61.8
(3) 		44.4
(-0) 		49.2
(0.8) 		44.7
(0) 		32.4
(-2.1) 		27.3
(-3) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 2.5
(-3)
A 		3.7
(-2.3)
A 		12.6
(3)
A 		7.7
(0.1)
A 		10
B 12
(0.3)
A 		21
(3)
A 		4.5
(-2)
A 		1.2
(-3)
A 		20
C 14.9
(3)
A 		3
(-3)
A 		10.2
(0.6)
A 		13.1
(2.1)
A 		12
avr 9.8
(3) 		9.2
(1.6) 		9.1
(1.3) 		7.3
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 9.5
(-0.4)
A 		11
(0.2)
A 		7
(-1.4)
A 		11.7
(0.5)
A 		17.8
(3)
A 		3
(-3)
A 		15
B 23.4
(3)
A 		15.5
(-0.4)
A 		9.3
(-3)
A 		11.5
(-2.1)
A 		14.1
(-1)
A 		10.8
(-2.4)
A 		14
C 24.6
(3)
A 		17
(1.1)
A 		19.6
(1.7)
A 		9
(-1)
A 		1.2
(-3)
A 		13.6
(0.2)
A 		23
avr 19.2
(3) 		14.5
(0.2) 		12
(-1.3) 		10.7
(-2) 		11
(-1.9) 		9.1
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177212_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18956
Sequence Type Exemplar sequence
Representative Public ID Y53G8AM.A NCBI
Target Description Y53G8AM.A /REP_DB=WormBase Gene ID /WP=CE22426 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59530.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065281
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065281 NCBI Caenorhabditis elegans Y53G8AM.8 (Y53G8AM.8) mRNA, complete cds. A 
Y53G8AM.8.2 ENSEMBL cdna:known chromosome:CEL140:III:3267167:3278687:-1 gene:Y53G8AM.8 A 
GENEFINDER00000032329 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3278291:3278699:-1 None
Y53G8AM.8.1 ENSEMBL cdna:known chromosome:CEL140:III:3267185:3278687:-1 gene:Y53G8AM.8 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3278290-3278648(-) 72.41 72.41
Public Domain and Genome References
Gene Title
Ensembl Y53G8AM.8
Entrez Gene 175430 Entrez gene
SwissProt Q9BL70 EMBL-EBI
WormBase CE27282 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29931 Hypothetical protein Y53G8AM.8 [Caenorhabditis elegans] ref|NP_497682.1| Y53G8AM.8 [Caenorhabditis elegans] 0.0
blast CAE69478 Hypothetical protein CBG15679 [Caenorhabditis briggsae] 1.0E-129
blast AAK29931 Hypothetical protein Y53G8AM.8 [Caenorhabditis elegans] ref|NP_497682.1| Y53G8AM.8 [Caenorhabditis elegans] 4.0E-42
Protein Domains
Database ID Description E-Value
scop a.4.1.Centromere-binding All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Centromere-binding 2.00000002337219E-7
Sequence
Target Sequence 
>C. ELEGANS:177212_AT
gtaccaacaactccatttaccattgaaaatacacctgtaggtagaggaaattctggtgct
agaagtcataaggagccaagcgcaaaacgtaaaaaacctaccagtacccctaccgtaaga
tgcggtaccgtaccccaagagccatccacttcagcagctacagtaacccaaaaaccagcg
ggatccaccatggccactgcaaacatcacggaaaccaggagaattgatataagcagttgt
agtgatcggatctctccagtagtatcgattgccatctggaccatccaggttgaatttctt
ctcgccggagaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTACCAACAACTCCATTTACCATTG 538 457 16 Antisense
GAAGTCATAAGGAGCCAAGCGCAAA 358 335 77 Antisense
CCAGTACCCCTACCGTAAGATGCGG 571 269 116 Antisense
TAAGATGCGGTACCGTACCCCAAGA 373 637 131 Antisense
CCAAAAACCAGCGGGATCCACCATG 254 275 183 Antisense
TGGCCACTGCAAACATCACGGAAAC 669 543 206 Antisense
AGCAGTTGTAGTGATCGGATCTCTC 3 79 247 Antisense
GGATCTCTCCAGTAGTATCGATTGC 2 513 263 Antisense
TATCGATTGCCATCTGGACCATCCA 361 657 278 Antisense
GACCATCCAGGTTGAATTTCTTCTC 475 383 294 Antisense
GTTGAATTTCTTCTCGCCGGAGAAA 68 437 304 Antisense
Sequence Source Affymetrix Proprietary Database