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[177211_at]

Description Y45G12B.2 /REP_DB=WormBase Gene ID /WP=CE21934 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60574.1
Cluster 6grp_total > 58      4grp_total > 51      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 204.3
(1.5)
A 		180.5
(0.6)
A 		244.5
(3)
A 		82.4
(-3)
A 		162
B 167.9
(-0.8)
A 		247
(3)
A 		186.4
(0.1)
A 		122
(-3)
A 		125
C 157.4
(-2.2)
A 		277.4
(3)
A 		196.1
(-0.5)
A 		138.7
(-3)
A 		139
avr 176.5
(0.1) 		235
(3) 		209
(1.7) 		114.4
(-3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 168.7
(-0.6)
A 		180.9
(-0.2)
A 		93.4
(-3)
A 		280
(3)
A 		119
(-2.2)
A 		255.8
(2.2)
A 		187
B 106.8
(2.4)
A 		90.3
(-1.6)
A 		84.3
(-3)
A 		109.3
(3)
A 		106.4
(2.3)
A 		93.6
(-0.8)
A 		25
C 235
(2.3)
P 		87.4
(-3)
A 		254.6
(3)
A 		89.3
(-2.9)
A 		209.6
(1.4)
A 		150.4
(-0.7)
A 		167
avr 170.2
(3) 		119.5
(-3) 		144.1
(-0.1) 		159.5
(1.7) 		145
(0) 		166.6
(2.6) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 74.4
(3)
A 		39.2
(-0.4)
A 		63.9
(2)
A 		12.1
(-3)
A 		62
B 53.5
(1.7)
A 		61
(3)
A 		43.2
(-0.1)
A 		26
(-3)
A 		35
C 52.1
(0.1)
A 		79.8
(3)
A 		68.3
(1.8)
A 		23.4
(-3)
A 		56
avr 60
(3) 		60
(3) 		58.5
(2.8) 		20.5
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 31.1
(-1.7)
A 		64.3
(2.2)
A 		20.3
(-3)
A 		71
(3)
A 		35.9
(-1.2)
A 		62.3
(2)
A 		51
B 37.1
(1.4)
A 		30.1
(-0.5)
A 		20.5
(-3)
A 		28.9
(-0.8)
A 		43.2
(3)
A 		34.9
(0.8)
A 		23
C 87.1
(3)
P 		26.7
(-2.2)
A 		64.8
(1.1)
A 		17.9
(-3)
A 		67.8
(1.3)
A 		50.3
(-0.2)
A 		69
avr 51.8
(3) 		40.4
(-1.1) 		35.2
(-3) 		39.3
(-1.5) 		49
(2) 		49.2
(2.1) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177211_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15172
Sequence Type Exemplar sequence
Representative Public ID Y45G12B.2 NCBI
Target Description Y45G12B.2 /REP_DB=WormBase Gene ID /WP=CE21934 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60574.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071330(11), NM_071331(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071330 NCBI Caenorhabditis elegans Y45G12B.2a (Y45G12B.2) mRNA, complete cds. 11/11 None
NM_071331 NCBI Caenorhabditis elegans Y45G12B.2b (Y45G12B.2) mRNA, complete cds. 11/11 None
GENEFINDER00000025517 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2694601:2698407:1 11/11 None
Y45G12B.2a ENSEMBL cdna:known chromosome:CEL140:V:2694595:2698665:1 gene:Y45G12B.2 11/11 None
Y45G12B.2b ENSEMBL cdna:known chromosome:CEL140:V:2695552:2698407:1 gene:Y45G12B.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2694600-2698407(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y45G12B.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60574 Hypothetical protein Y45G12B.2a [Caenorhabditis elegans] ref|NP_503731.1| Y45G12B.2a [Caenorhabditis elegans] 1.0E-178
blast CAE62535 Hypothetical protein CBG06644 [Caenorhabditis briggsae] 1.0E-150
blast AAK70665 Hypothetical protein Y45G12B.2b [Caenorhabditis elegans] ref|NP_503732.1| Y45G12B.2b [Caenorhabditis elegans] 1.0E-135
blast AAK70665 Hypothetical protein Y45G12B.2b [Caenorhabditis elegans] ref|NP_503732.1| Y45G12B.2b [Caenorhabditis elegans] 1.0E-129
blast AAF60574 Hypothetical protein Y45G12B.2a [Caenorhabditis elegans] ref|NP_503731.1| Y45G12B.2a [Caenorhabditis elegans] 1.0E-129
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503731.1 1 255-274
NP_503732.1 1 192-211
Y45G12B.2a 1 255-274
Y45G12B.2b 1 192-211
GENEFINDER00000025517 2 104-126,330-349
Sequence
Target Sequence 
>C. ELEGANS:177211_AT
tcccaccgatcaatgaagtctctccactcatcgagaagctccgcgagcaactgaaacagt
ccaactgggcaagagcagcgctcgggctcccgactcttccggaactcaatcggccagtgc
cttctccggcgccgccacagctgaaaaatgctccagttatgcataaagaggtcccggtgc
acaataatagatcctccacccccgcaacccatctggaaatggaagacaccgcttcctact
cggtctccaacaatgacgtcacttttgcgcgaaaaaagaactacggagccgaatcttcaa
gcgatactcgtccacttttacagctccgtgacgccgataacgaggagaataaatataaac
ggcgcccgacgatggattggatgcgcgggctctggagagcaaaacatggcggctccgggg
tcccacaggaacgggcttccgcgaaaaaaatcgcgcttttcgtgattttcctggccgttt
tggctttgattacgattataatggtgatgaagcgtgccggatacagcggcgagcactcat
cggacccccttttcgatccaatggccaatccgaatattcgcgtcgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCACCGATCAATGAAGTCTCTCC 65 599 326 Antisense
AAGTCTCTCCACTCATCGAGAAGCT 592 155 341 Antisense
CCAACTGGGCAAGAGCAGCGCTCGG 282 271 386 Antisense
CCGACTCTTCCGGAACTCAATCGGC 623 263 415 Antisense
GGAGCCGAATCTTCAAGCGATACTC 374 521 610 Antisense
CGTCCACTTTTACAGCTCCGTGACG 172 245 634 Antisense
TAAACGGCGCCCGACGATGGATTGG 126 637 681 Antisense
TTTTCCTGGCCGTTTTGGCTTTGAT 427 677 791 Antisense
GCGTGCCGGATACAGCGGCGAGCAC 578 291 837 Antisense
CCCTTTTCGATCCAATGGCCAATCC 352 255 872 Antisense
GGCCAATCCGAATATTCGCGTCGCC 111 543 888 Antisense
Sequence Source Affymetrix Proprietary Database