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[177209_at]

Description Y73B6BL.U /REP_DB=WormBase Gene ID /WP=CE26293 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23479.1
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 48      4grp_highest_8_8 > 01      4grp_high_8_8 > 44      6grp_highest_nonorm > 01      6grp_total > 48      4grp_total > 51      6grp_highest_sub > Cu      6grp_cgc > 17      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2759
1 2 3 4 range
A 1798.8
(-0.4)
P 		3228.1
(3)
P 		2372
(1)
P 		695.7
(-3)
P 		2532
B 1790.1
(-0.8)
P 		3262.6
(3)
P 		2477.9
(1)
P 		935.6
(-3)
P 		2327
C 1510.8
(-1.3)
P 		3454.5
(3)
P 		2560.6
(1)
P 		747.7
(-3)
P 		2707
avr 1699.9
(-0.8) 		3315.1
(3) 		2470.2
(1) 		793
(-3) 		2522

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3089
1 2 3 4 5 6 range
A 2612
(0.8)
P 		1970.9
(-0.5)
P 		781.1
(-3)
P 		1109.7
(-2.3)
P 		977
(-2.6)
P 		3642.5
(3)
P 		2861
B 2995.3
(3)
P 		1285
(-1.6)
P 		929.9
(-2.5)
P 		744.6
(-3)
P 		1095
(-2.1)
P 		2362.3
(1.3)
P 		2251
C 3833.8
(3)
P 		1301
(-2)
P 		1361.8
(-1.9)
P 		824
(-3)
P 		1525.9
(-1.6)
P 		2339.3
(0)
P 		3010
avr 3147
(3) 		1519
(-1.3) 		1024.3
(-2.6) 		892.8
(-3) 		1199.3
(-2.2) 		2781.4
(2) 		2254
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 891
1 2 3 4 range
A 654.9
(2.5)
P 		700.7
(3)
P 		620.1
(2.2)
P 		101.8
(-3)
P 		599
B 570.9
(0.7)
P 		805.8
(3)
P 		574.2
(0.7)
P 		199.4
(-3)
P 		606
C 500.1
(-0.4)
P 		993.2
(3)
P 		892.4
(2.3)
P 		126.1
(-3)
P 		867
avr 575.3
(0.8) 		833.2
(3) 		695.6
(1.8) 		142.4
(-3) 		691

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1255
1 2 3 4 5 6 range
A 481.5
(-0.4)
P 		701
(1.4)
P 		170.1
(-3)
P 		281.4
(-2.1)
P 		294.8
(-2)
P 		886.8
(3)
P 		717
B 1042.2
(3)
P 		427.7
(-1.4)
P 		225.8
(-2.8)
P 		197.2
(-3)
P 		444.9
(-1.2)
P 		880.9
(1.9)
P 		845
C 1421
(3)
P 		397
(-1.9)
P 		346.6
(-2.1)
P 		165.7
(-3)
P 		493.8
(-1.4)
P 		783.1
(-0)
P 		1255
avr 981.6
(3) 		508.6
(-0.7) 		247.5
(-2.7) 		214.8
(-3) 		411.2
(-1.5) 		850.3
(2) 		767
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177209_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19124
Sequence Type Exemplar sequence
Representative Public ID Y73B6BL.U NCBI
Target Description Y73B6BL.U /REP_DB=WormBase Gene ID /WP=CE26293 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23479.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171356(11), AF017811(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171356 NCBI Caenorhabditis elegans HIstone H1 Like family member (hil-2) (hil-2) mRNA, complete cds. 11/11 None
AF017811 NCBI Caenorhabditis elegans histone H1.2 mRNA, complete cds. 9/11 None
SNAP00000032026 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6294110:6295057:1 11/11 None
Y73B6BL.9a ENSEMBL cdna:known chromosome:CEL140:IV:6294110:6295193:1 gene:Y73B6BL.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171909 4/11 Cross Hyb Matching Probes None
GENEFINDER00000032088 8/11 Cross Hyb Matching Probes None
Y73B6BL.9b 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177390 2 1 NM_171356 177209_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6294115-6295063(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y73B6BL.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM44399 Histone h1 like protein 2, isoform a [Caenorhabditis elegans] ref|NP_741417.3| HIstone H1 Like family member (hil-2) [Caenorhabditis elegans] 3.0E-67
blast AAM44399 Histone h1 like protein 2, isoform a [Caenorhabditis elegans] ref|NP_741417.3| HIstone H1 Like family member (hil-2) [Caenorhabditis elegans] 2.0E-36
blast P15796 Histone H1.2 (Histone H1-like protein 2) gb|AAB70666.1| histone H1.2 [Caenorhabditis elegans] gb|AAB70559.1| histone H1.2 [Caenorhabditis elegans] 2.0E-36
blast P15796 Histone H1.2 (Histone H1-like protein 2) gb|AAB70666.1| histone H1.2 [Caenorhabditis elegans] gb|AAB70559.1| histone H1.2 [Caenorhabditis elegans] 4.0E-36
blast AAN63427 Histone h1 like protein 2, isoform b [Caenorhabditis elegans] ref|NP_741418.1| HIstone H1 Like family member (hil-2) [Caenorhabditis elegans] 3.0E-32
Protein Domains
Database ID Description E-Value
scop a.4.5.Histone H1/H5 All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Histone H1/H5 5.00000001813747E-16
scop a.4.5.Histone H1/H5 All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Histone H1/H5 7.00000013127157E-15
Pfam IPR005818 EMBL-EBI Histone H1/H5 6.9E-35
Pfam IPR005818 EMBL-EBI Histone H1/H5 6.9E-35
Sequence
Target Sequence 
>C. ELEGANS:177209_AT
accatcactttccaagtgtccacgcatcgttctacaatgtctgacgtcaccgttgccgag
actccagcagtcaagaccccaaccaaggctccaaaagctccaaagtccaagactaccaaa
gagccaaaggctaaggtcgctgctgctcatccaccattcatcaacatggttaccgctgcc
atcagcagcctcaaggagcgcaagggatcctccaagatcgccatcttgaagtacatcacc
gccaactacaaggtcggagatcagctcaccaagatcaactctcgcctccgcgccgcactc
aacaagggagtcgccagcaaggcactcgtccaatccgtcggaaatggagccagtggacga
ttccgtgttgctgagaaggctgccgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCATCACTTTCCAAGTGTCCACGC 93 87 127 Antisense
TGTCCACGCATCGTTCTACAATGTC 515 557 143 Antisense
CTACAATGTCTGACGTCACCGTTGC 415 227 158 Antisense
GCCAAAGGCTAAGGTCGCTGCTGCT 405 277 249 Antisense
ATCAACATGGTTACCGCTGCCATCA 285 27 286 Antisense
CGCCATCTTGAAGTACATCACCGCC 683 255 345 Antisense
CAAGGTCGGAGATCAGCTCACCAAG 629 183 375 Antisense
GCGCCGCACTCAACAAGGGAGTCGC 15 289 416 Antisense
CACTCGTCCAATCCGTCGGAAATGG 429 197 449 Antisense
AATGGAGCCAGTGGACGATTCCGTG 170 163 469 Antisense
TCCGTGTTGCTGAGAAGGCTGCCGC 377 595 488 Antisense
Sequence Source Affymetrix Proprietary Database