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[177208_at]

Description Y53G8AM.D /REP_DB=WormBase Gene ID /WP=CE25413 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59533.1
Cluster 6grp_total > 90      4grp_total > 29      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 75.8
(-0.8)
A 		54.3
(-1.7)
A 		26.8
(-3)
A 		158.1
(3)
P 		131
B 120.8
(3)
P 		75.8
(-0.9)
A 		51.1
(-3)
A 		98
(1)
A 		70
C 101.5
(3)
A 		73.1
(0.7)
M 		27.3
(-3)
A 		58.8
(-0.5)
P 		74
avr 99.4
(2.5) 		67.7
(-0.2) 		35.1
(-3) 		105
(3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 59.4
(1.6)
A 		66.6
(2.2)
A 		4.1
(-3)
A 		76.1
(3)
A 		73
(2.7)
A 		48.9
(0.7)
M 		72
B 95.6
(3)
A 		69.7
(0.4)
A 		35.9
(-3)
A 		73.1
(0.7)
A 		46.4
(-1.9)
A 		49.1
(-1.7)
A 		60
C 94.4
(3)
P 		62.7
(0.4)
A 		22.5
(-3)
A 		79
(1.7)
A 		72.5
(1.2)
A 		56.2
(-0.2)
A 		72
avr 83.1
(3) 		66.3
(1.4) 		20.8
(-3) 		76.1
(2.3) 		64
(1.2) 		51.4
(-0.1) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 27.6
(3)
A 		11.8
(-1.6)
A 		7
(-3)
A 		23.1
(1.7)
P 		21
B 38.5
(3)
P 		18.7
(-1.4)
A 		11.8
(-3)
A 		20.9
(-1)
A 		27
C 33.6
(3)
A 		21
(-0.1)
M 		9.5
(-3)
A 		9.9
(-2.9)
P 		24
avr 33.2
(3) 		17.2
(-1) 		9.4
(-3) 		18
(-0.8) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 10.9
(-0.4)
A 		23.7
(3)
A 		0.9
(-3)
A 		19.3
(1.8)
A 		22
(2.6)
A 		11.9
(-0.1)
M 		23
B 33.3
(3)
A 		23.2
(0.5)
A 		8.7
(-3)
A 		19.3
(-0.4)
A 		18.8
(-0.5)
A 		18.3
(-0.7)
A 		25
C 35
(3)
P 		19.1
(-0.3)
A 		5.7
(-3)
A 		15.9
(-0.9)
A 		23.5
(0.6)
A 		18.8
(-0.3)
A 		29
avr 26.4
(3) 		22
(1.8) 		5.1
(-3) 		18.2
(0.7) 		21.4
(1.6) 		16.3
(0.2) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177208_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18969
Sequence Type Exemplar sequence
Representative Public ID Y53G8AM.D NCBI
Target Description Y53G8AM.D /REP_DB=WormBase Gene ID /WP=CE25413 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59533.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065280(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065280 NCBI Caenorhabditis elegans Y53G8AM.7 (Y53G8AM.7) mRNA, complete cds. 11/11 None
GENEFINDER00000032328 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3261919:3266408:-1 10/11 None
SNAP00000032322 ENSEMBL cdna:SNAP chromosome:CEL140:III:3263671:3266597:-1 10/11 None
Y53G8AM.7 ENSEMBL cdna:known chromosome:CEL140:III:3263671:3266597:-1 gene:Y53G8AM.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3263670-3266597(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8AM.7
Entrez Gene 190239 Entrez gene
SwissProt Q9N3G8 EMBL-EBI
WormBase CE25413 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29924 Hypothetical protein Y53G8AM.7 [Caenorhabditis elegans] ref|NP_497681.1| Y53G8AM.7 [Caenorhabditis elegans] 1.0E-118
blast AAK29924 Hypothetical protein Y53G8AM.7 [Caenorhabditis elegans] ref|NP_497681.1| Y53G8AM.7 [Caenorhabditis elegans] 6.0E-94
blast AAK29924 Hypothetical protein Y53G8AM.7 [Caenorhabditis elegans] ref|NP_497681.1| Y53G8AM.7 [Caenorhabditis elegans] 9.0E-93
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497681.1 6 13-35,50-72,85-103,118-143,156-178,188-210
Y53G8AM.7 6 13-35,50-72,85-103,118-143,156-178,188-210
GENEFINDER00000032328 4 21-43,58-80,93-115,125-147
SNAP00000032322 5 15-37,46-68,88-110,123-145,155-177
Sequence
Target Sequence 
>C. ELEGANS:177208_AT
gacaatttctcttatcactgggctactgattatgggaaccgcagagttttgcgcggtgtt
caacatgaatccaaggtgccatccggaattgaaggtttgctggtggatctacaacatact
tctagccatagtcacactgttaagatttgtaccccaaattgtcatgctatgtctggatgg
gtttgatgtacagataatctacatcccaatgataatattcaacacctcatccgccctctc
catctcaatttgcttcgggaatctcctcatcgaagacattttccgtttcaaaatccgtct
caccataatcctcgtcaccctaatcaccacaatcactctcaatctcatcgatctcgtcgt
cgccgcctttttcatccaaaaaatcaataaatcaataagcgaatcgacgtggttctacgt
gtcatgcggtctgcttactgtaatcccattgctcttcggatatttcgtcgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAATTTCTCTTATCACTGGGCTA 77 365 171 Antisense
GAATCCAAGGTGCCATCCGGAATTG 68 329 237 Antisense
TACTTCTAGCCATAGTCACACTGTT 494 645 287 Antisense
CTCTCCATCTCAATTTGCTTCGGGA 578 231 406 Antisense
TTGCTTCGGGAATCTCCTCATCGAA 369 703 420 Antisense
TTCAAAATCCGTCTCACCATAATCC 478 671 457 Antisense
CAATCACTCTCAATCTCATCGATCT 341 181 500 Antisense
GACGTGGTTCTACGTGTCATGCGGT 306 377 576 Antisense
GTCATGCGGTCTGCTTACTGTAATC 438 465 591 Antisense
TACTGTAATCCCATTGCTCTTCGGA 132 647 606 Antisense
ATTGCTCTTCGGATATTTCGTCGCC 211 7 618 Antisense
Sequence Source Affymetrix Proprietary Database