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[177205_at]

Description M88.4 /REP_DB=WormBase Gene ID /WP=CE23885 /TR=Q21602 /GB=CAA84335.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 46      4grp_total > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 98.4
(-3)
A 		103
(-1)
A 		103.9
(-0.7)
A 		112.5
(3)
A 		14
B 113.9
(3)
A 		55.9
(-1.2)
A 		102.4
(2.2)
A 		30.2
(-3)
A 		84
C 77.1
(-2.6)
A 		93.3
(-1.3)
A 		147.8
(3)
A 		72.3
(-3)
A 		76
avr 96.5
(0.2) 		84.1
(-1.4) 		118
(3) 		71.7
(-3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 109
(2.7)
A 		87.3
(0.6)
A 		112.2
(3)
A 		83
(0.2)
A 		49.1
(-3)
A 		57.3
(-2.2)
A 		63
B 74.2
(0.8)
A 		100.1
(2.6)
A 		17.2
(-3)
A 		54
(-0.5)
A 		76.5
(1)
A 		106.4
(3)
A 		89
C 37.7
(-2.2)
A 		146
(3)
A 		20
(-3)
A 		57.4
(-1.2)
A 		50.8
(-1.5)
A 		96.9
(0.7)
A 		126
avr 73.6
(-0.7) 		111.1
(3) 		49.8
(-3) 		64.8
(-1.5) 		58.8
(-2.1) 		86.9
(0.6) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 35.8
(3)
A 		22.4
(-1.2)
A 		27.2
(0.3)
A 		16.5
(-3)
A 		19
B 36.3
(3)
A 		13.8
(-1.5)
A 		23.7
(0.5)
A 		6.4
(-3)
A 		30
C 25.5
(-1)
A 		26.8
(-0.8)
A 		51.5
(3)
A 		12.2
(-3)
A 		39
avr 32.5
(2.6) 		21
(-0.5) 		34.1
(3) 		11.7
(-3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 20.1
(-0.8)
A 		31
(3)
A 		24.4
(0.7)
A 		21
(-0.5)
A 		14.8
(-2.7)
A 		13.9
(-3)
A 		17
B 25.8
(0.7)
A 		33.3
(1.9)
A 		4.2
(-3)
A 		14.3
(-1.3)
A 		31.1
(1.5)
A 		39.7
(3)
A 		36
C 14
(-1.6)
A 		44.5
(3)
A 		5.1
(-3)
A 		11.5
(-2)
A 		16.4
(-1.3)
A 		32.4
(1.2)
A 		39
avr 20
(-0.9) 		36.3
(3) 		11.2
(-3) 		15.6
(-1.9) 		20.8
(-0.7) 		28.7
(1.2) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177205_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6538
Sequence Type Exemplar sequence
Representative Public ID M88.4 NCBI
Target Description M88.4 /REP_DB=WormBase Gene ID /WP=CE23885 /TR=Q21602 /GB=CAA84335.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065521(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065521 NCBI Caenorhabditis elegans M88.4 (M88.4) mRNA, complete cds. 9/11 None
GENEFINDER00000031351 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4549060:4551439:1 11/11 None
SNAP00000031341 ENSEMBL cdna:SNAP chromosome:CEL140:III:4549216:4551439:1 9/11 None
M88.4 ENSEMBL cdna:known chromosome:CEL140:III:4549028:4551470:1 gene:M88.4 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4549059-4551439(+) 99.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M88.4
Entrez Gene 175594 Entrez gene
SwissProt Q21602 EMBL-EBI
WormBase CE34207 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84335 Hypothetical protein M88.4 [Caenorhabditis elegans] ref|NP_497922.2| M88.4 [Caenorhabditis elegans] 0.0
blast CAA84335 Hypothetical protein M88.4 [Caenorhabditis elegans] ref|NP_497922.2| M88.4 [Caenorhabditis elegans] 1.0E-165
blast CAE71187 Hypothetical protein CBG18044 [Caenorhabditis briggsae] 1.0E-160
blast CAE71187 Hypothetical protein CBG18044 [Caenorhabditis briggsae] 1.0E-156
blast CAE71187 Hypothetical protein CBG18044 [Caenorhabditis briggsae] 1.0E-136
blast AAS65293 CG12075-PB, isoform B [Drosophila melanogaster] gb|AAF46414.3| CG12075-PA, isoform A [Drosophila melanogaster] ref|NP_572506.2| CG12075-PA, isoform A [Drosophila melanogaster] ref|NP_996386.1| CG12075-PB, isoform B [Drosophila melanogaster] 5.0E-23
Sequence
Target Sequence 
>C. ELEGANS:177205_AT
tgctctctcgacctgatgtacaaagatctattaacgatttggagcatcggttattcttgt
caggactattagttccaagaccacccagacggctggcgccaacgatttcaaggagagtcg
tcgatgaattgaaaaacaaaataatcggagaatcacgaatcggaaatatgagtcggagtc
gagaggtgagtctgggtcttgccacgaacaaagttgttttccgaacagagctcgtcgcgc
aagaaggtagctctttgaatcgatatgagtcagaaaaacgagcatcattatcagagctcc
caattgaaacagactcttcacgatatagaatgtttggagacacaatgctacgagatacaa
aaaacaaatcgagaagtttggacgatttggcagctgatccacttgtttccacaaatcttc
gcggcagtgatctggcaccaccaccaccgcgccgattcccacgatctgaatatgtggctg
acccttttgggcacaactcctacgaacgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCTCTCGACCTGATGTACAAAG 552 581 437 Antisense
GGAGCATCGGTTATTCTTGTCAGGA 335 521 477 Antisense
TCGAGAGGTGAGTCTGGGTCTTGCC 289 603 615 Antisense
AACAGAGCTCGTCGCGCAAGAAGGT 659 135 660 Antisense
GCATCATTATCAGAGCTCCCAATTG 238 309 718 Antisense
GGACGATTTGGCAGCTGATCCACTT 324 521 816 Antisense
GATCCACTTGTTTCCACAAATCTTC 526 415 832 Antisense
AATCTTCGCGGCAGTGATCTGGCAC 255 169 850 Antisense
GCCGATTCCCACGATCTGAATATGT 44 285 887 Antisense
GAATATGTGGCTGACCCTTTTGGGC 577 325 904 Antisense
TTTGGGCACAACTCCTACGAACGGA 2 663 922 Antisense
Sequence Source Affymetrix Proprietary Database