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[177195_s_at]

Description Y38F2AL.D /REP_DB=WormBase Gene ID /WP=CE21620 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59474.1
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 60      6grp_total > 06      4grp_total > 60      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 750
1 2 3 4 range
A 681.3
(1.6)
P 		843.9
(3)
P 		732.9
(2)
P 		146.7
(-3)
P 		697
B 706.6
(2.7)
P 		728
(3)
P 		726
(3)
P 		243.5
(-3)
P 		485
C 801.5
(2.2)
P 		772.3
(2)
P 		896.4
(3)
P 		166.4
(-3)
P 		730
avr 729.8
(2.4) 		781.4
(3) 		785.1
(3) 		185.5
(-3) 		600

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1259
1 2 3 4 5 6 range
A 324.6
(-1.3)
P 		748.3
(3)
P 		316.2
(-1.4)
P 		396.3
(-0.6)
P 		156.8
(-3)
P 		259.7
(-2)
P 		592
B 374.1
(2)
P 		418.7
(3)
P 		356.3
(1.6)
P 		142.8
(-3)
P 		235.5
(-1)
P 		148.6
(-2.9)
P 		276
C 1401.3
(3)
P 		296.2
(-2.3)
P 		568.3
(-1)
P 		150.6
(-3)
P 		434.9
(-1.6)
P 		208.2
(-2.7)
P 		1251
avr 700
(3) 		487.7
(0.4) 		413.6
(-0.5) 		229.9
(-2.7) 		275.7
(-2.1) 		205.5
(-3) 		495
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 291
1 2 3 4 range
A 248.1
(3)
P 		183.2
(1.3)
P 		191.6
(1.5)
P 		21.5
(-3)
P 		227
B 225.3
(3)
P 		179.8
(1.4)
P 		168.2
(1)
P 		51.9
(-3)
P 		173
C 265.3
(2)
P 		222
(1.1)
P 		312.4
(3)
P 		28.1
(-3)
P 		284
avr 246.2
(3) 		195
(1.6) 		224.1
(2.4) 		33.8
(-3) 		212

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 489
1 2 3 4 5 6 range
A 59.8
(-2.7)
P 		266.1
(3)
P 		68.9
(-2.4)
P 		100.5
(-1.5)
P 		47.3
(-3)
P 		63.2
(-2.6)
P 		219
B 130.2
(2.5)
P 		139.4
(3)
P 		86.5
(-0.1)
P 		37.8
(-3)
P 		95.7
(0.4)
P 		55.4
(-2)
P 		102
C 519.4
(3)
P 		90.4
(-2.3)
P 		144.6
(-1.6)
P 		30.3
(-3)
P 		140.7
(-1.6)
P 		69.7
(-2.5)
P 		489
avr 236.5
(3) 		165.3
(0.6) 		100
(-1.5) 		56.2
(-3) 		94.6
(-1.7) 		62.8
(-2.8) 		180
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177195_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19388
Sequence Type Exemplar sequence
Representative Public ID Y38F2AL.D NCBI
Target Description Y38F2AL.D /REP_DB=WormBase Gene ID /WP=CE21620 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59474.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028280(11), NM_001028281(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028280 NCBI Caenorhabditis elegans Vacuolar H ATPase family member (vha-11) (vha-11) mRNA, complete cds. 11/11 None
NM_001028281 NCBI Caenorhabditis elegans Vacuolar H ATPase family member (vha-11) (vha-11) mRNA, complete cds. 11/11 None
SNAP00000023928 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2308245:2315944:-1 11/11 None
GENEFINDER00000023935 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2308245:2314660:-1 11/11 None
Y38F2AL.3a ENSEMBL cdna:known chromosome:CEL140:IV:2307816:2314566:-1 gene:Y38F2AL.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Y38F2AL.3b 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2313519-2314539(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y38F2AL.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023451 Vacuolar H ATPase family member (vha-11) [Caenorhabditis elegans] gb|AAF59474.3| Vacuolar h atpase protein 11, isoform a [Caenorhabditis elegans] sp|Q9XXU9|VATC_CAEEL Vacuolar ATP synthase subunit C (V-ATPase C subunit) (Vacuolar proton pump C subunit) dbj|BAA75067.1| Vha11 protein [Caenorhabditis elegans] 0.0
blast CAE70305 Hypothetical protein CBG16826 [Caenorhabditis briggsae] 0.0
blast AAH83532 Atp6v1c1l protein [Danio rerio] ref|XP_686493.1| PREDICTED: similar to Atp6v1c1l protein [Danio rerio] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR004907 EMBL-EBI V-ATPase subunit C 1.0E-126
Pfam IPR004907 EMBL-EBI V-ATPase subunit C 2.8E-54
Pfam IPR004907 EMBL-EBI V-ATPase subunit C 1.0E-126
Pfam IPR002379 EMBL-EBI H+-transporting two-sector ATPase, C subunit 2.7E-16
Pfam IPR002379 EMBL-EBI H+-transporting two-sector ATPase, C subunit 5.5E-22
Pfam IPR004907 EMBL-EBI V-ATPase subunit C 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000023928 4 15-37,58-78,98-120,133-155
Sequence
Target Sequence 
>C. ELEGANS:177195_S_AT
cgacgtccggcgaatattggcttatttcggtgcccggtgagaagggagcaaatgatgcgt
gggataagctcaatcgatccactggaaatacgtcgaccaactcgaaatatttgattcctg
atttgaaggtcggaaccttggatcaactcgtcggattatcggatgatttgagcaagttgg
acacctctgctgaggcagttattcgcaaacttgtgcaatatttcaccgaagttctcgagg
aagataagagcaaaattgccgagaatttggtcattggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACGTCCGGCGAATATTGGCTTAT 290 251 23 Antisense
TATTGGCTTATTTCGGTGCCCGGTG 616 665 37 Antisense
TTCGGTGCCCGGTGAGAAGGGAGCA 117 1 48 Antisense
ATAAGCTCAATCGATCCACTGGAAA 533 25 86 Antisense
ACTGGAAATACGTCGACCAACTCGA 411 97 103 Antisense
AGGTCGGAACCTTGGATCAACTCGT 98 57 149 Antisense
GATCAACTCGTCGGATTATCGGATG 61 423 163 Antisense
AGCAAGTTGGACACCTCTGCTGAGG 389 75 193 Antisense
AGTTATTCGCAAACTTGTGCAATAT 444 63 219 Antisense
TGCAATATTTCACCGAAGTTCTCGA 598 575 236 Antisense
ATTGCCGAGAATTTGGTCATTGGAA 144 7 277 Antisense
Sequence Source Affymetrix Proprietary Database