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[177193_s_at]

Description AC3.3 /REP_DB=WormBase Gene ID /WP=CE05133 /TR=Q17400 /GB=CAA94867.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 76      6grp_total > 79      4grp_total > 49      6grp_cgc > 58      6grp_high1_sub > Da      6grp_cgc_sub > In      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2341
1 2 3 4 range
A 96.5
(-3)
A 		2401.5
(3)
P 		2102.9
(2.2)
P 		110.3
(-3)
P 		2305
B 95.4
(-2.9)
A 		2253
(2.9)
P 		2299.7
(3)
P 		60.9
(-3)
A 		2239
C 91.2
(-3)
A 		2194.4
(3)
P 		2049.7
(2.6)
P 		211.3
(-2.7)
P 		2103
avr 94.4
(-3) 		2283
(3) 		2150.8
(2.6) 		127.5
(-2.9) 		2189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2958
1 2 3 4 5 6 range
A 163.8
(-3)
M 		1731.6
(0.2)
P 		652
(-2)
P 		669.4
(-2)
P 		1454.5
(-0.3)
P 		3078.2
(3)
P 		2914
B 157.2
(-3)
P 		1599.2
(0.3)
P 		933.1
(-1.2)
P 		719.6
(-1.7)
P 		1268
(-0.4)
P 		2752.3
(3)
P 		2595
C 120
(-3)
P 		1631.3
(0.4)
P 		782.7
(-1.5)
P 		682.3
(-1.7)
P 		1685.1
(0.5)
P 		2817.5
(3)
P 		2698
avr 147
(-3) 		1654
(0.3) 		789.3
(-1.6) 		690.4
(-1.8) 		1469.2
(-0.1) 		2882.7
(3) 		2736
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 701
1 2 3 4 range
A 35.1
(-2.8)
A 		521.3
(2.7)
P 		549.7
(3)
P 		16.1
(-3)
P 		534
B 30.4
(-2.8)
A 		556.4
(3)
P 		532.9
(2.7)
P 		13
(-3)
A 		543
C 30.2
(-3)
A 		630.9
(2.3)
P 		714.4
(3)
P 		35.6
(-3)
P 		684
avr 31.9
(-2.9) 		569.5
(2.7) 		599
(3) 		21.6
(-3) 		577

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 996
1 2 3 4 5 6 range
A 30.2
(-3)
M 		615.8
(1.9)
P 		142
(-2.1)
P 		169.8
(-1.8)
P 		438.9
(0.4)
P 		749.4
(3)
P 		719
B 54.7
(-3)
P 		532.3
(-0.1)
P 		226.6
(-1.9)
P 		190.6
(-2.2)
P 		515.2
(-0.2)
P 		1026.3
(3)
P 		972
C 44.5
(-3)
P 		497.7
(0)
P 		199.2
(-2)
P 		137.2
(-2.4)
P 		545.3
(0.3)
P 		943.2
(3)
P 		899
avr 43.1
(-3) 		548.6
(0.5) 		189.3
(-2) 		165.9
(-2.1) 		499.8
(0.2) 		906.3
(3) 		863
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177193_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11348
Sequence Type Exemplar sequence
Representative Public ID AC3.3 NCBI
Target Description AC3.3 /REP_DB=WormBase Gene ID /WP=CE05133 /TR=Q17400 /GB=CAA94867.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073266(11), NM_073267(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073266 NCBI Caenorhabditis elegans Activated in Blocked Unfolded protein response family member (abu-1) (abu-1) mRNA, complete cds. 11/11 None
NM_073267 NCBI Caenorhabditis elegans Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-2) (pqn-2) mRNA, complete cds. 11/11 A 
SNAP00000028834 ENSEMBL cdna:SNAP chromosome:CEL140:V:10383008:10384367:-1 11/11 None
SNAP00000028835 ENSEMBL cdna:SNAP chromosome:CEL140:V:10385060:10386558:1 11/11 None
GENEFINDER00000028848 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10384562:10386558:1 11/11 None
GENEFINDER00000028852 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10383008:10384367:-1 11/11 None
AC3.3 ENSEMBL cdna:known chromosome:CEL140:V:10382921:10384405:-1 gene:AC3.3 11/11 None
AC3.4 ENSEMBL cdna:known chromosome:CEL140:V:10385060:10386558:1 gene:AC3.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070442 1/11 Cross Hyb Matching Probes A 
NM_070443 1/11 Cross Hyb Matching Probes A 
SNAP00000030809 1/11 Cross Hyb Matching Probes None
GENEFINDER00000030899 1/11 Cross Hyb Matching Probes None
Y73F8A.8 1/11 Cross Hyb Matching Probes A 
Y73F8A.9 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10385059-10386558(+) 94.91 97.5
chrV:10383007-10384367(-) 97.57 100.0
Public Domain and Genome References
Gene Title

Gene Symbol abu-1
pqn-2
Ensembl AC3.3
AC3.4
Entrez Gene 181800 Entrez gene
181801 Entrez gene
SwissProt Q17400 EMBL-EBI
Q17401 EMBL-EBI
WormBase CE05133 Wormbase
CE37872 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.22268.1.S1_X_AT similar to keratin associated protein 10-8 cfa
CANINE_2:CFAAFFX.22273.1.S1_X_AT similar to keratin associated protein 10-8 cfa
CANINE_2:CFAAFFX.23307.1.S1_AT similar to keratin associated protein 10-8 cfa
Protein Similarities
Method ID Description E-Value
blast CAA94868 Hypothetical protein AC3.4 [Caenorhabditis elegans] ref|NP_505668.2| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-2) [Caenorhabditis elegans] 3.0E-51
blast CAA94867 Hypothetical protein AC3.3 [Caenorhabditis elegans] ref|NP_505667.1| Activated in Blocked Unfolded protein response family member (abu-1) [Caenorhabditis elegans] 2.0E-47
blast CAB54985 Hypothetical protein Y105C5A.6 [Caenorhabditis elegans] emb|CAB54982.1| Hypothetical protein Y105C5A.3 [Caenorhabditis elegans] ref|NP_502875.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-79) [Caenorhabditis elegans] ref|NP_502872.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-76) [Caenorhabditis elegans] 2.0E-43
blast CAA94868 Hypothetical protein AC3.4 [Caenorhabditis elegans] ref|NP_505668.2| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-2) [Caenorhabditis elegans] 2.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR009475 EMBL-EBI Protein of unknown function DUF1096 1.6E-38
Pfam IPR009475 EMBL-EBI Protein of unknown function DUF1096 4.8E-38
Pfam IPR009475 EMBL-EBI Protein of unknown function DUF1096 3.4E-27
Sequence
Target Sequence 
>C. ELEGANS:177193_S_AT
aatcagcaatgcacccagcaatgccagaccacctgccagcaagcagttccacaatgccaa
caacagtgtgctccacaatgccaacaaccatcagctccacagtgccagcagtgccagaat
acctgccaacaagctgctccagtttgtcagcaacagtgtgctccacaatgtcaacagcaa
tctgcaccagcttgccaacaatgtcagacttcgtgccaacaaactcaacagtgccaacaa
caatgtactccacagtgccagcaaccatctgctccacaatgccagcaatgccaatctgca
tgccaagctccagttgccactactgctgctccacaagttgtcacaatcattctggaagct
tcagtctcccaatctgcacaatgtgaaccacaatgccagcaatcatgccaacagcaatgc
gttcagcagcaacaaccaatgcaacaatgtgctccagcttgcacccaatcttgctctcaa
tcctgttctgctgctcaaccagcccagatgccatgccagacacaatcagtcaactcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCAGCAATGCACCCAGCAATGCC 137 171 694 Antisense
GCTCCACAATGTCAACAGCAATCTG 509 297 853 Antisense
CAATGTCAACAGCAATCTGCACCAG 356 183 859 Antisense
AATGTCAGACTTCGTGCCAACAAAC 647 163 893 Antisense
GCCAACAACAATGTACTCCACAGTG 661 277 926 Antisense
CAACAATGTACTCCACAGTGCCAGC 388 189 931 Antisense
CAATCTGCATGCCAAGCTCCAGTTG 638 181 985 Antisense
TGTCACAATCATTCTGGAAGCTTCA 100 559 1032 Antisense
TTCTGGAAGCTTCAGTCTCCCAATC 271 695 1043 Antisense
CCAGATGCCATGCCAGACACAATCA 520 269 1197 Antisense
ATGCCAGACACAATCAGTCAACTCC 291 41 1206 Antisense
Sequence Source Affymetrix Proprietary Database