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[177184_at]

Description M28.2 /REP_DB=WormBase Gene ID /WP=CE02281 /TR=Q21572 /GB=CAA90130.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 62      4grp_total > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 3.7
(-3)
A 		4.2
(-3)
A 		6.2
(-2.8)
A 		71.8
(3)
A 		68
B 4.6
(-3)
A 		11.5
(1.1)
A 		14.8
(3)
A 		6.1
(-2.1)
A 		10
C 5.7
(-2.9)
A 		3.4
(-3)
A 		3.5
(-3)
A 		155.5
(3)
P 		152
avr 4.7
(-3) 		6.4
(-2.9) 		8.2
(-2.7) 		77.8
(3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 5.7
(-2.3)
A 		1.2
(-3)
A 		39.5
(3)
A 		7.8
(-2)
A 		12.4
(-1.2)
A 		5.6
(-2.3)
A 		38
B 4.8
(-3)
A 		6.8
(-2.6)
A 		14.6
(-1.2)
A 		37.4
(3)
A 		13
(-1.5)
A 		7.1
(-2.6)
A 		33
C 4.3
(-2.8)
A 		11.3
(3)
A 		4
(-3)
A 		6.9
(-0.6)
A 		7.8
(0.1)
A 		4.7
(-2.4)
A 		7
avr 4.9
(-3) 		6.4
(-2.4) 		19.4
(3) 		17.4
(2.2) 		11.1
(-0.4) 		5.8
(-2.6) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 1.4
(-2.7)
A 		0.9
(-3)
A 		1.6
(-2.6)
A 		10.5
(3)
A 		10
B 1.5
(-2.4)
A 		2.8
(1.3)
A 		3.4
(3)
A 		1.3
(-3)
A 		2
C 1.9
(-2.8)
A 		1
(-3)
A 		1.2
(-3)
A 		26.2
(3)
P 		25
avr 1.6
(-3) 		1.6
(-3) 		2.1
(-2.7) 		12.7
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 1.1
(-2.5)
A 		0.4
(-3)
A 		8.6
(3)
A 		2
(-1.8)
A 		3.7
(-0.6)
A 		1.4
(-2.3)
A 		8
B 1.7
(-3)
A 		2.3
(-2.6)
A 		3.5
(-1.7)
A 		9.9
(3)
A 		5.3
(-0.4)
A 		2.6
(-2.3)
A 		8
C 1.6
(-1.6)
A 		3.5
(3)
A 		1
(-3)
A 		1.4
(-2)
A 		2.5
(0.6)
A 		1.6
(-1.6)
A 		3
avr 1.5
(-3) 		2.1
(-1.8) 		4.4
(3) 		4.4
(3) 		3.8
(1.8) 		1.9
(-2.2) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177184_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3372
Sequence Type Exemplar sequence
Representative Public ID M28.2 NCBI
Target Description M28.2 /REP_DB=WormBase Gene ID /WP=CE02281 /TR=Q21572 /GB=CAA90130.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063894(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063894 NCBI Caenorhabditis elegans M28.2 (M28.2) mRNA, complete cds. 11/11 None
GENEFINDER00000007413 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10638131:10640396:1 9/11 None
M28.2 ENSEMBL cdna:known chromosome:CEL140:II:10633387:10639023:1 gene:M28.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000007402 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10633383-10639020(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M28.2
Entrez Gene 187462 Entrez gene
SwissProt Q21572 EMBL-EBI
WormBase CE02281 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90130 Hypothetical protein M28.2 [Caenorhabditis elegans] ref|NP_496295.1| M28.2 [Caenorhabditis elegans] 0.0
blast CAA90129 Hypothetical protein M28.9 [Caenorhabditis elegans] ref|NP_496296.1| M28.9 [Caenorhabditis elegans] 1.0E-165
blast CAA90132 Hypothetical protein M28.8 [Caenorhabditis elegans] ref|NP_496294.1| M28.8 [Caenorhabditis elegans] 1.0E-109
blast CAA90129 Hypothetical protein M28.9 [Caenorhabditis elegans] ref|NP_496296.1| M28.9 [Caenorhabditis elegans] 3.0E-84
blast CAA90130 Hypothetical protein M28.2 [Caenorhabditis elegans] ref|NP_496295.1| M28.2 [Caenorhabditis elegans] 7.0E-68
blast CAA90132 Hypothetical protein M28.8 [Caenorhabditis elegans] ref|NP_496294.1| M28.8 [Caenorhabditis elegans] 6.0E-33
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496295.1 5 98-120,141-163,387-409,421-443,609-631
M28.2 5 98-120,141-163,387-409,421-443,609-631
GENEFINDER00000007413 4 85-107,215-232,316-338,359-381
Sequence
Target Sequence 
>C. ELEGANS:177184_AT
atctgcgaatctggctatctgtgacaacaataatttagatcaaacgtttattgcatactc
ggaatctctttccatgtcccacttactttcccagcaatttgtcaaaaaattagagactct
ggcagaaaatgctccgaaaacgccatcgattgcaaaagatctgaataaaacttatttcac
taaaacggaagcaattttgaaacaggctgttaccaagctttttaaagccctgcaagatga
tattttcaagtgtcgcccgttagtagatgtctacaataatgcagggatttcagtttgcga
gcaatttggattaccagttcatggaatgtgggcatcggttggcttgggtggtgttgtgct
attcttccaaataattttattgcttcttacttttcgttggttgagaactcatagcaagaa
tactgttggcaagaatggaaaaagtgataagacggggacatcaggctcaatggaaaaggc
tggctcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCTGCGAATCTGGCTATCTGTGAC 232 33 1512 Antisense
GTTTATTGCATACTCGGAATCTCTT 367 447 1557 Antisense
CACTTACTTTCCCAGCAATTTGTCA 370 197 1591 Antisense
GAAACAGGCTGTTACCAAGCTTTTT 207 357 1710 Antisense
GTGTCGCCCGTTAGTAGATGTCTAC 308 487 1761 Antisense
GCAGGGATTTCAGTTTGCGAGCAAT 149 311 1792 Antisense
GAATGTGGGCATCGGTTGGCTTGGG 350 331 1835 Antisense
GTGGTGTTGTGCTATTCTTCCAAAT 359 489 1859 Antisense
ATTGCTTCTTACTTTTCGTTGGTTG 471 3 1890 Antisense
ATAAGACGGGGACATCAGGCTCAAT 573 5 1958 Antisense
GGCTCAATGGAAAAGGCTGGCTCGA 609 537 1975 Antisense
Sequence Source Affymetrix Proprietary Database