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[177179_at]

Description Y61A9LA.G /REP_DB=WormBase Gene ID /WP=CE25523 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60839.1
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 10      4grp_high_8_8 > 27      6grp_highest_nonorm > 90      6grp_total > 40      4grp_total > 29      6grp_cgc > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 82.8
(3)
P 		29.3
(-3)
A 		53.7
(-0.3)
P 		76.9
(2.3)
A 		54
B 68.6
(0.9)
P 		88.1
(3)
P 		55.4
(-0.4)
P 		31.1
(-3)
P 		57
C 101.6
(0.7)
P 		81.6
(-0.8)
P 		52.8
(-3)
P 		131.5
(3)
P 		79
avr 84.3
(3) 		66.3
(-0.6) 		54
(-3) 		79.8
(2.1) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 100.7
(3)
P 		80.5
(0.3)
P 		66.8
(-1.5)
P 		68.7
(-1.2)
P 		55.5
(-3)
P 		64.3
(-1.8)
P 		45
B 55
(-1.9)
P 		66.3
(0.3)
P 		79.6
(3)
P 		78.7
(2.8)
P 		54.7
(-2)
P 		49.6
(-3)
P 		30
C 95.6
(2)
P 		89
(1.3)
P 		86.4
(1.1)
P 		105.8
(3)
P 		68.6
(-0.7)
P 		45.4
(-3)
P 		60
avr 83.8
(2.9) 		78.6
(1.9) 		77.6
(1.7) 		84.4
(3) 		59.6
(-1.8) 		53.1
(-3) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 30.2
(3)
P 		6.4
(-3)
A 		14
(-1.1)
P 		11.3
(-1.8)
A 		24
B 21.9
(3)
P 		21.8
(3)
P 		12.8
(-0.6)
P 		6.6
(-3)
P 		15
C 33.6
(3)
P 		23.5
(-1)
P 		18.4
(-3)
P 		22.2
(-1.5)
P 		15
avr 28.6
(3) 		17.2
(-1.5) 		15.1
(-2.3) 		13.4
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 18.6
(-1.3)
P 		28.6
(3)
P 		14.5
(-3)
P 		17.4
(-1.8)
P 		16.7
(-2.1)
P 		15.6
(-2.5)
P 		14
B 19.1
(-2)
P 		22.1
(2.8)
P 		19.3
(-1.7)
P 		20.8
(0.7)
P 		22.2
(3)
P 		18.5
(-3)
P 		4
C 35.5
(3)
P 		27.2
(0.5)
P 		22
(-1)
P 		21.3
(-1.2)
P 		22.2
(-0.9)
P 		15.2
(-3)
P 		20
avr 24.4
(2) 		26
(3) 		18.6
(-1.6) 		19.8
(-0.9) 		20.4
(-0.5) 		16.4
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177179_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19534
Sequence Type Exemplar sequence
Representative Public ID Y61A9LA.G NCBI
Target Description Y61A9LA.G /REP_DB=WormBase Gene ID /WP=CE25523 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60839.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071834(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071834 NCBI Caenorhabditis elegans Drosophila SOS homolog family member (sos-1) (sos-1) mRNA, complete cds. 10/11 None
SNAP00000010090 ENSEMBL cdna:SNAP chromosome:CEL140:V:4536006:4548884:-1 10/11 None
GENEFINDER00000010113 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4536618:4545194:-1 10/11 None
T28F12.3 ENSEMBL cdna:known chromosome:CEL140:V:4517496:4549105:-1 gene:T28F12.3 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4536867-4549084(-) 97.1 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sos-1
Ensembl T28F12.3
Entrez Gene 178846 Entrez gene
SwissProt Q9N5D3 EMBL-EBI
WormBase CE36328 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1600356_AT similar to son of sevenless homolog 1 cfa
CANINE_2:CFA.13872.1.A1_AT similar to son of sevenless homolog 1 cfa
CANINE_2:CFAAFFX.10491.1.S1_S_AT similar to son of sevenless homolog 1 cfa
CANINE_2:CFAAFFX.10496.1.S1_S_AT similar to son of sevenless homolog 1 cfa
CHICKEN:GGAAFFX.8419.1.S1_S_AT similar to son of sevenless homolog 1; gingival fibromatosis, hereditary, 1; gingival fibromatosis gga
CHICKEN:GGAAFFX.8419.3.S1_S_AT similar to son of sevenless homolog 1; gingival fibromatosis, hereditary, 1; gingival fibromatosis gga
CHICKEN:GGAAFFX.8419.5.S1_S_AT similar to son of sevenless homolog 1; gingival fibromatosis, hereditary, 1; gingival fibromatosis gga
HG-U133_PLUS_2:212777_AT son of sevenless homolog 1 (Drosophila) hs
HG-U133A_2:212777_AT son of sevenless homolog 1 (Drosophila) hs
HG-U133A:212777_AT son of sevenless homolog 1 (Drosophila) hs
HU35KSUBA:N25110_AT son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS.326392.1.A2_3P_AT son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS.326392.1.A1_3P_AT son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS.285655.0.A1_3P_AT son of sevenless homolog 1 (Drosophila) hs
HG-U133A_2:212780_AT son of sevenless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:212780_AT son of sevenless homolog 1 (Drosophila) hs
HG-U133A:212780_AT son of sevenless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:230337_AT son of sevenless homolog 1 (Drosophila) hs
HG-U133B:230337_AT son of sevenless homolog 1 (Drosophila) hs
HG-U95AV2:33260_AT son of sevenless homolog 1 (Drosophila) hs
HG-U95E:85839_AT son of sevenless homolog 1 (Drosophila) hs
HU35KSUBC:RC_N66706_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBD:RC_N26906_AT Son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS2.175444.1.A1_3P_AT Son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS.270247.0.S1_3P_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:1557354_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:232883_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133B:232883_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95B:50604_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95C:56196_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95E:71624_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:227426_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133B:227426_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:229261_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133B:229261_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:242018_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133B:242018_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:242682_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U133B:242682_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95B:45917_R_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95B:46678_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95C:50375_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95B:58423_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95C:58618_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95C:60439_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95C:61750_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95E:67035_R_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95D:69068_AT Son of sevenless homolog 1 (Drosophila) hs
HG-U95D:84509_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBB:RC_H91313_S_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBB:RC_R87650_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBB:RC_T23860_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBC:RC_AA287008_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBD:RC_AA292478_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBD:RC_N32963_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBD:RC_T54672_AT Son of sevenless homolog 1 (Drosophila) hs
HU35KSUBD:RC_W74256_AT Son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS.109080.0.A1_3P_AT Son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS.285655.1.A1_3P_AT Son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS.293345.0.S1_3P_AT Son of sevenless homolog 1 (Drosophila) hs
U133_X3P:HS.7312.0.A1_3P_AT Son of sevenless homolog 1 (Drosophila) hs
MU11KSUBB:Z11574_S_AT Son of sevenless homolog 1 (Drosophila) mm
MG-U74AV2:160911_AT Son of sevenless homolog 1 (Drosophila) mm
MOE430A:1421884_AT Son of sevenless homolog 1 (Drosophila) mm
MOUSE430A_2:1421884_AT Son of sevenless homolog 1 (Drosophila) mm
MOUSE430_2:1421884_AT Son of sevenless homolog 1 (Drosophila) mm
MOE430A:1421885_AT Son of sevenless homolog 1 (Drosophila) mm
MOUSE430A_2:1421885_AT Son of sevenless homolog 1 (Drosophila) mm
MOUSE430_2:1421885_AT Son of sevenless homolog 1 (Drosophila) mm
MOE430A:1421886_AT Son of sevenless homolog 1 (Drosophila) mm
MOUSE430A_2:1421886_AT Son of sevenless homolog 1 (Drosophila) mm
MOUSE430_2:1421886_AT Son of sevenless homolog 1 (Drosophila) mm
MG-U74BV2:114094_AT Son of sevenless homolog 1 (Drosophila) (Sos1), mRNA mm
MU19KSUBA:TC17682_AT Son of sevenless homolog 1 (Drosophila) (Sos1), mRNA mm
MU19KSUBB:TC34416_AT Son of sevenless homolog 1 (Drosophila) (Sos1), mRNA mm
MU19KSUBC:TC41718_S_AT Son of sevenless homolog 1 (Drosophila) (Sos1), mRNA mm
RAE230A:1389710_AT Son of sevenless homolog 1 (Drosophila) (predicted) rn
RAT230_2:1389710_AT Son of sevenless homolog 1 (Drosophila) (predicted) rn
RG-U34A:RC_AA892860_AT Son of sevenless homolog 1 (Drosophila) (predicted) rn
RG-U34A:RC_AA892860_G_AT Son of sevenless homolog 1 (Drosophila) (predicted) rn
RG-U34C:RC_AI176851_AT Son of sevenless homolog 1 (Drosophila) (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5085 guanyl-nucleotide exchange factor activity inferred from electronic annotation QuickGO AmiGO
5088 Ras guanyl-nucleotide exchange factor activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF39972 Drosophila sos homolog protein 1 [Caenorhabditis elegans] ref|NP_504235.2| Drosophila SOS homolog family member (sos-1) [Caenorhabditis elegans] 0.0
blast AAF82360 guanine nucleotide exchange factor for RAS [Caenorhabditis elegans] 0.0
blast CAE56769 Hypothetical protein CBG24573 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 8.7E-50
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 1.2E-16
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.2E-12
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 8.7E-50
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 1.2E-16
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.2E-12
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 1.5E-9
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 9.9E-30
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 1.2E-16
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.2E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000010113 1 1119-1138
Sequence
Target Sequence 
>C. ELEGANS:177179_AT
taacctactgggtatcccgatcaatagtggaaacggaatccctcgaagaacgtatggcaa
tgttcaacagagtacttgaagtgatgtcagtattcgaagaacttcataatttcacgggtc
tagtcgccttctactctgcactcaattcttcgtgtattttccgattaaaatggtgttggg
atgggttggataacgagaagaagaagtgtttcgatcgattcaatacactttgtgaacggc
gttggcaagaaatgcagaaacgtctcagttcgattaatcccccgtgtattccgttcttcg
gtcattatctttccaatatttattttttggagcaaggtaacagcacatttgtcaacaagt
caccaccacatggtgctgccggtgcacaaaaacagcagaaggacgatttgaaggcatcgg
taagtggagaggagaaggcaggtgtgcctctttttgagtttccggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAACCTACTGGGTATCCCGATCAAT 666 637 3722 Antisense
GGAATCCCTCGAAGAACGTATGGCA 326 529 3756 Antisense
ATAATTTCACGGGTCTAGTCGCCTT 349 25 3827 Antisense
GCACTCAATTCTTCGTGTATTTTCC 662 315 3859 Antisense
ATACACTTTGTGAACGGCGTTGGCA 490 23 3944 Antisense
GAAACGTCTCAGTTCGATTAATCCC 510 357 3978 Antisense
CGTGTATTCCGTTCTTCGGTCATTA 253 245 4004 Antisense
TTCTTCGGTCATTATCTTTCCAATA 112 691 4015 Antisense
TTTGTCAACAAGTCACCACCACATG 471 665 4069 Antisense
CACATGGTGCTGCCGGTGCACAAAA 116 197 4088 Antisense
TGTGCCTCTTTTTGAGTTTCCGGAC 532 555 4164 Antisense
Sequence Source Affymetrix Proprietary Database