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[177175_at]

Description Y61A9LA.D /REP_DB=WormBase Gene ID /WP=CE22673 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60836.1
Cluster 6grp_total > 70      4grp_total > 42      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 64.1
(3)
A 		37.5
(-0.4)
A 		21.4
(-2.5)
A 		17.2
(-3)
A 		47
B 5.7
(-3)
A 		27.9
(-0.2)
A 		54
(3)
A 		13.1
(-2.1)
A 		48
C 7.2
(-3)
A 		70.6
(3)
A 		19.6
(-1.8)
A 		52.9
(1.3)
A 		63
avr 25.7
(-3) 		45.3
(3) 		31.7
(-1.2) 		27.7
(-2.4) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 18.4
(-1.9)
A 		18.9
(-1.9)
A 		18.8
(-1.9)
A 		69.2
(3)
A 		7.5
(-3)
A 		10.6
(-2.7)
A 		62
B 48.5
(3)
A 		32.7
(0.9)
A 		16.8
(-1.2)
A 		45
(2.5)
A 		2.9
(-3)
A 		37.1
(1.5)
A 		46
C 16.2
(1.5)
A 		4.2
(-3)
A 		20.3
(3)
A 		16.8
(1.7)
A 		11.4
(-0.3)
A 		17.9
(2.1)
A 		16
avr 27.7
(0.4) 		18.6
(-1.1) 		18.6
(-1.1) 		43.7
(3) 		7.3
(-3) 		21.9
(-0.6) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 23.3
(3)
A 		8.1
(-1.4)
A 		5.6
(-2.1)
A 		2.5
(-3)
A 		21
B 1.8
(-3)
A 		6.9
(-0.1)
A 		12.5
(3)
A 		2.8
(-2.4)
A 		11
C 2.4
(-3)
A 		20.3
(3)
A 		6.8
(-1.5)
A 		8.9
(-0.8)
A 		18
avr 9.2
(0.8) 		11.8
(3) 		8.3
(0) 		4.7
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 3.4
(-2.6)
A 		6.7
(-1.3)
A 		4.1
(-2.3)
A 		17.6
(3)
A 		2.3
(-3)
A 		2.6
(-2.9)
A 		15
B 16.9
(3)
A 		10.9
(0.7)
A 		4.1
(-1.9)
A 		11.9
(1.1)
A 		1.2
(-3)
A 		13.8
(1.8)
A 		16
C 6
(3)
A 		1.3
(-3)
A 		5.2
(2)
A 		3.4
(-0.3)
A 		3.7
(0.1)
A 		6
(3)
A 		5
avr 8.8
(1.5) 		6.3
(-0.3) 		4.5
(-1.5) 		11
(3) 		2.4
(-3) 		7.5
(0.6) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177175_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19528
Sequence Type Exemplar sequence
Representative Public ID Y61A9LA.D NCBI
Target Description Y61A9LA.D /REP_DB=WormBase Gene ID /WP=CE22673 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60836.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071841(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071841 NCBI Caenorhabditis elegans Y61A9LA.9 (Y61A9LA.9) mRNA, complete cds. 11/11 A 
SNAP00000010098 ENSEMBL cdna:SNAP chromosome:CEL140:V:4572947:4579544:-1 11/11 None
GENEFINDER00000010109 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4572947:4573738:-1 11/11 None
Y61A9LA.9 ENSEMBL cdna:known chromosome:CEL140:V:4572947:4573738:-1 gene:Y61A9LA.9 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000005511 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1586038-1586731(+) 86.87 86.87
chrV:4572946-4573738(-) 80.68 100.0
Public Domain and Genome References
Gene Title
Ensembl Y61A9LA.9
Entrez Gene 190447 Entrez gene
SwissProt Q9N312 EMBL-EBI
WormBase CE22673 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60836 Hypothetical protein Y61A9LA.9 [Caenorhabditis elegans] ref|NP_504242.1| Y61A9LA.9 [Caenorhabditis elegans] 1.0E-115
blast NP_508294 H02F09.4 [Caenorhabditis elegans] 1.0E-17
blast AAG23472 Hypothetical protein Y73B6BL.16 [Caenorhabditis elegans] ref|NP_500972.1| Y73B6BL.16 [Caenorhabditis elegans] 1.0E-13
Sequence
Target Sequence 
>C. ELEGANS:177175_AT
ttgctcactacccgtgttgaagaattggaaaaggaaaattcgagcttgagaaccttccgt
accgccgctgaaagcagatgtcgtgccctggagagcaaacaatcggaactcttccaaacc
gtcgagcagctcaaggtagttgttgccaagctcacgaacgaatgctctgcccgtgttacc
tccaatttgcccgctccacgcctgttcgccgatgttctcaagggacaatcagccgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCTCACTACCCGTGTTGAAGAAT 79 703 175 Antisense
ATTCGAGCTTGAGAACCTTCCGTAC 532 5 212 Antisense
CCGTACCGCCGCTGAAAGCAGATGT 275 265 231 Antisense
TGAAAGCAGATGTCGTGCCCTGGAG 559 569 243 Antisense
GCCCTGGAGAGCAAACAATCGGAAC 110 285 259 Antisense
CAATCGGAACTCTTCCAAACCGTCG 453 179 274 Antisense
TCCAAACCGTCGAGCAGCTCAAGGT 571 587 287 Antisense
GCAGCTCAAGGTAGTTGTTGCCAAG 292 313 300 Antisense
GTTGTTGCCAAGCTCACGAACGAAT 78 437 313 Antisense
CTGTTCGCCGATGTTCTCAAGGGAC 358 239 376 Antisense
TGTTCTCAAGGGACAATCAGCCGAG 192 563 387 Antisense
Sequence Source Affymetrix Proprietary Database