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[177164_s_at]

Description Y45G5AM.D /REP_DB=WormBase Gene ID /WP=CE26401 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23361.1
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 51      4grp_highest_8_8 > 02      4grp_high_8_8 > 54      6grp_highest_nonorm > 62      6grp_total > 72      4grp_total > 56      6grp_highest_sub > 04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 324
1 2 3 4 range
A 485.9
(-2.8)
P 		729.1
(3)
P 		669.8
(1.6)
P 		478.1
(-3)
P 		251
B 460.6
(-3)
P 		482.2
(-1.8)
P 		539.8
(1.3)
P 		570
(3)
P 		109
C 508.8
(-0.5)
P 		584.9
(1.4)
P 		650.4
(3)
P 		405.2
(-3)
P 		245
avr 485.1
(-3) 		598.7
(2.1) 		620
(3) 		484.4
(-3) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 500
1 2 3 4 5 6 range
A 365.8
(-0.4)
P 		602.2
(3)
P 		556.7
(2.3)
P 		603.9
(3)
P 		526.2
(1.9)
P 		184.6
(-3)
P 		419
B 401.5
(-1.3)
P 		598.6
(1.7)
P 		684.1
(3)
P 		620
(2)
P 		514.2
(0.4)
P 		292.6
(-3)
P 		392
C 280.4
(-2.6)
P 		539.8
(1.2)
P 		662.8
(3)
P 		558.9
(1.5)
P 		420.9
(-0.5)
P 		250.8
(-3)
P 		412
avr 349.2
(-1.4) 		580.2
(2.2) 		634.5
(3) 		594.3
(2.4) 		487.1
(0.7) 		242.7
(-3) 		392
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 176.9
(3)
P 		158.3
(2)
P 		175.1
(2.9)
P 		70
(-3)
P 		107
B 146.9
(3)
P 		119.1
(-3)
P 		125.1
(-1.7)
P 		121.5
(-2.5)
P 		28
C 168.4
(0.8)
P 		168.1
(0.8)
P 		226.7
(3)
P 		68.3
(-3)
P 		158
avr 164.1
(2.2) 		148.5
(1.2) 		175.6
(3) 		86.6
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 67.4
(-2.2)
P 		214.2
(3)
P 		121.2
(-0.3)
P 		153.2
(0.8)
P 		158.8
(1)
P 		45
(-3)
P 		169
B 139.7
(-1.2)
P 		199.3
(2.4)
P 		166.1
(0.4)
P 		164.2
(0.3)
P 		208.9
(3)
P 		109.1
(-3)
P 		100
C 103.9
(-1.6)
P 		164.7
(2.7)
P 		168.7
(3)
P 		112.4
(-1)
P 		136.2
(0.7)
P 		83.9
(-3)
P 		85
avr 103.7
(-1.7) 		192.7
(3) 		152
(0.8) 		143.3
(0.4) 		168
(1.7) 		79.3
(-3) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177164_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19470
Sequence Type Exemplar sequence
Representative Public ID Y45G5AM.D NCBI
Target Description Y45G5AM.D /REP_DB=WormBase Gene ID /WP=CE26401 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23361.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071755(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071755 NCBI Caenorhabditis elegans Y45G5AM.6 (Y45G5AM.6) mRNA, complete cds. 11/11 A 
SNAP00000024438 ENSEMBL cdna:SNAP chromosome:CEL140:V:4147520:4153765:-1 11/11 None
GENEFINDER00000024461 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4147520:4150798:-1 11/11 None
Y45G5AM.6 ENSEMBL cdna:known chromosome:CEL140:V:4147426:4152085:-1 gene:Y45G5AM.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4147519-4152036(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y45G5AM.6
Entrez Gene 178815 Entrez gene
SwissProt Q9GUJ2 EMBL-EBI
WormBase CE27407 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504156 Y45G5AM.6 [Caenorhabditis elegans] gb|AAG23361.2| Hypothetical protein Y45G5AM.6 [Caenorhabditis elegans] 0.0
blast NP_504156 Y45G5AM.6 [Caenorhabditis elegans] gb|AAG23361.2| Hypothetical protein Y45G5AM.6 [Caenorhabditis elegans] 1.0E-176
blast CAE58333 Hypothetical protein CBG01446 [Caenorhabditis briggsae] 1.0E-172
blast CAE58333 Hypothetical protein CBG01446 [Caenorhabditis briggsae] 1.0E-156
blast NP_504156 Y45G5AM.6 [Caenorhabditis elegans] gb|AAG23361.2| Hypothetical protein Y45G5AM.6 [Caenorhabditis elegans] 1.0E-153
blast CAE58333 Hypothetical protein CBG01446 [Caenorhabditis briggsae] 1.0E-138
Sequence
Target Sequence 
>C. ELEGANS:177164_S_AT
agggttgcatgaatgtttccgacaagacacatatccaaatcggttgctccaaaaaatgga
tatccgacgaatacgaagaaattcactgtgcatgtcgtggaaatctttgcaatagcgact
ccttgactgccacgtcaccaaaattgctccaaatcgttcacgtgttgctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGGTTGCATGAATGTTTCCGACAA 588 57 911 Antisense
GCATGAATGTTTCCGACAAGACACA 491 309 917 Antisense
GTTTCCGACAAGACACATATCCAAA 267 451 925 Antisense
GACACATATCCAAATCGGTTGCTCC 124 367 936 Antisense
AAATGGATATCCGACGAATACGAAG 508 119 964 Antisense
GGATATCCGACGAATACGAAGAAAT 225 511 968 Antisense
GAAGAAATTCACTGTGCATGTCGTG 316 339 985 Antisense
AATTCACTGTGCATGTCGTGGAAAT 58 177 990 Antisense
CCACGTCACCAAAATTGCTCCAAAT 345 275 1040 Antisense
ATTGCTCCAAATCGTTCACGTGTTG 192 7 1053 Antisense
CTCCAAATCGTTCACGTGTTGCTGC 696 233 1057 Antisense
Sequence Source Affymetrix Proprietary Database