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[177157_at]

Description R53.8 /REP_DB=WormBase Gene ID /WP=CE03578 /TR=Q22026 /GB=CAA88572.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 83      4grp_total > 21      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 3.6
(-2.9)
A 		3.3
(-3)
A 		4.4
(-2.8)
A 		29.7
(3)
A 		26
B 34.4
(3)
A 		7.8
(-2.3)
A 		4.5
(-3)
A 		13.9
(-1.1)
A 		30
C 27.8
(-0.7)
A 		31.3
(-0.1)
A 		14.2
(-3)
A 		50.1
(3)
A 		36
avr 21.9
(0.6) 		14.1
(-1.4) 		7.7
(-3) 		31.2
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 12.1
(-1.4)
A 		3.1
(-3)
A 		17.9
(-0.4)
A 		5.8
(-2.5)
A 		37.6
(3)
A 		26.3
(1)
A 		35
B 10.6
(-2.1)
A 		8.2
(-3)
A 		24.7
(3)
A 		13
(-1.3)
A 		17.8
(0.5)
A 		23.3
(2.5)
A 		17
C 3.9
(-3)
A 		23.8
(0.4)
A 		20.7
(-0.1)
A 		38.9
(3)
A 		19.4
(-0.3)
A 		4.3
(-2.9)
A 		35
avr 8.9
(-3) 		11.7
(-2) 		21.1
(1.6) 		19.2
(0.9) 		24.9
(3) 		18
(0.4) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 1.3
(-2)
A 		0.7
(-3)
A 		1.2
(-2.2)
A 		4.3
(3)
A 		4
B 11
(3)
A 		1.9
(-2.5)
A 		1
(-3)
A 		3
(-1.8)
A 		10
C 9.2
(3)
A 		9
(2.7)
A 		4.9
(-3)
A 		8.4
(1.9)
A 		4
avr 7.2
(3) 		3.9
(-1.1) 		2.4
(-3) 		5.2
(0.5) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 2.2
(-2.4)
A 		1.1
(-3)
A 		3.9
(-1.4)
A 		1.5
(-2.8)
A 		11.3
(3)
A 		6.4
(0.1)
A 		10
B 3.7
(-2)
A 		2.7
(-3)
A 		6
(0.3)
A 		3.4
(-2.3)
A 		7.2
(1.5)
A 		8.7
(3)
A 		6
C 1.4
(-3)
A 		7.3
(2.5)
A 		5.3
(0.7)
A 		7.8
(3)
A 		6.3
(1.6)
A 		1.4
(-3)
A 		6
avr 2.4
(-3) 		3.7
(-1.7) 		5.1
(-0.3) 		4.2
(-1.2) 		8.3
(3) 		5.5
(0.2) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177157_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3734
Sequence Type Exemplar sequence
Representative Public ID R53.8 NCBI
Target Description R53.8 /REP_DB=WormBase Gene ID /WP=CE03578 /TR=Q22026 /GB=CAA88572.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063755(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063755 NCBI Caenorhabditis elegans R53.8 (R53.8) mRNA, complete cds. 11/11 None
R53.8 ENSEMBL cdna:known chromosome:CEL140:II:9976616:9977743:1 gene:R53.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9976612-9977740(+) 85.77 100.0
Public Domain and Genome References
Gene Title
Ensembl R53.8
Entrez Gene 187886 Entrez gene
SwissProt Q22026 EMBL-EBI
WormBase CE03578 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88572 Hypothetical protein R53.8 [Caenorhabditis elegans] emb|CAA91355.1| Hypothetical protein R53.8 [Caenorhabditis elegans] ref|NP_496156.1| R53.8 [Caenorhabditis elegans] 3.0E-73
blast CAE57934 Hypothetical protein CBG00988 [Caenorhabditis briggsae] 5.0E-33
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496156.1 1 13-35
R53.8 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:177157_AT
gatgttcattattttactctccgtagccttatgttcaacatcactagccgctcctctcaa
tgtggaatatgttcttgcacccgtcccactgaagcaaagcacatcagacaaatcaaaaat
gatagcaattgtgcctcaaaacacgacaaaaatcgtcataaagtttgacaaaccgatttt
ggccgaaatcgagaagtcatcggatttcgaagcaaattttgaggcgaaagtttttgaaaa
ggagtccaaaattgtgaagttgaaagcgaaaggcgaactgaaaaagccggttatcgttag
tattaaagacaagaaaaatggaaattcggttaaagttatggtcaaggtggaagatcaaga
atcagcggatcagcagaagaaggatcaggagaagaaggaacaagatggagacaagaaaga
caaggagaaggttagcgagaaaccgaaggaacaaccggaggatcaaccgaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTTCATTATTTTACTCTCCGTA 85 411 48 Antisense
TCTCCGTAGCCTTATGTTCAACATC 305 609 65 Antisense
TTATGTTCAACATCACTAGCCGCTC 355 679 76 Antisense
GCCGCTCCTCTCAATGTGGAATATG 651 285 94 Antisense
TGTGGAATATGTTCTTGCACCCGTC 678 553 108 Antisense
CCGTCCCACTGAAGCAAAGCACATC 190 265 128 Antisense
AGCAATTGTGCCTCAAAACACGACA 60 77 171 Antisense
GAAGTCATCGGATTTCGAAGCAAAT 287 337 240 Antisense
GGCGAACTGAAAAAGCCGGTTATCG 446 537 319 Antisense
GAATCAGCGGATCAGCAGAAGAAGG 709 325 406 Antisense
GAACAACCGGAGGATCAACCGAAAG 82 353 496 Antisense
Sequence Source Affymetrix Proprietary Database