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[177154_at]

Description Y73B6BL.G /REP_DB=WormBase Gene ID /WP=CE26280 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23466.1
Cluster 6grp_total > 66      4grp_total > 01      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 9.6
(-2.1)
A 		2.5
(-3)
A 		3.2
(-2.9)
A 		48.7
(3)
A 		46
B 5.6
(-2.7)
A 		4.1
(-3)
A 		26.4
(1.2)
A 		35.7
(3)
A 		32
C 3.7
(-2.7)
A 		2.5
(-2.9)
A 		1.7
(-3)
A 		36.6
(3)
A 		35
avr 6.3
(-2.5) 		3
(-3) 		10.4
(-1.8) 		40.3
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 1.8
(-3)
A 		10.6
(-0.6)
A 		7.8
(-1.3)
A 		1.8
(-3)
A 		3.9
(-2.4)
A 		23.6
(3)
A 		22
B 1.1
(-3)
A 		9.3
(3)
A 		1.7
(-2.6)
A 		2.7
(-1.8)
A 		4.8
(-0.3)
A 		9.1
(2.9)
A 		8
C 7.3
(-0.7)
A 		2.8
(-2.8)
A 		2.4
(-3)
A 		15.4
(3)
A 		5
(-1.8)
A 		4
(-2.3)
A 		13
avr 3.4
(-3) 		7.6
(-0.1) 		4
(-2.6) 		6.6
(-0.8) 		4.6
(-2.2) 		12.2
(3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 3.5
(-0.3)
A 		0.5
(-3)
A 		0.8
(-2.7)
A 		7.1
(3)
A 		7
B 1.8
(-2.3)
A 		1
(-3)
A 		6.1
(1.6)
A 		7.6
(3)
A 		7
C 1.2
(-2.4)
A 		0.7
(-2.9)
A 		0.6
(-3)
A 		6.2
(3)
A 		6
avr 2.2
(-1.6) 		0.7
(-3) 		2.5
(-1.3) 		7
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 0.3
(-3)
A 		3.8
(0.9)
A 		1.7
(-1.4)
A 		0.5
(-2.8)
A 		1.2
(-2)
A 		5.7
(3)
A 		5
B 0.4
(-3)
A 		3.1
(2.4)
A 		0.4
(-3)
A 		0.7
(-2.4)
A 		2
(0.2)
A 		3.4
(3)
A 		3
C 2.7
(2)
A 		0.9
(-2.3)
A 		0.6
(-3)
A 		3.1
(3)
A 		1.6
(-0.6)
A 		1.3
(-1.3)
A 		3
avr 1.1
(-2.5) 		2.6
(0.9) 		0.9
(-3) 		1.4
(-1.8) 		1.6
(-1.4) 		3.5
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177154_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19153
Sequence Type Exemplar sequence
Representative Public ID Y73B6BL.G NCBI
Target Description Y73B6BL.G /REP_DB=WormBase Gene ID /WP=CE26280 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23466.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068578(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068578 NCBI Caenorhabditis elegans Y73B6BL.22 (Y73B6BL.22) mRNA, complete cds. 11/11 None
SNAP00000032056 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6416237:6416601:-1 11/11 None
Y73B6BL.22 ENSEMBL cdna:known chromosome:CEL140:IV:6416237:6416601:-1 gene:Y73B6BL.22 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6416242-6416607(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y73B6BL.22
Entrez Gene 190655 Entrez gene
SwissProt Q9GUF4 EMBL-EBI
WormBase CE26280 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG23466 Hypothetical protein Y73B6BL.22 [Caenorhabditis elegans] ref|NP_500979.1| Y73B6BL.22 [Caenorhabditis elegans] 6.0E-55
blast CAE61544 Hypothetical protein CBG05450 [Caenorhabditis briggsae] 1.0E-26
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500979.1 1 47-69
Y73B6BL.22 1 47-69
SNAP00000032056 1 47-69
Sequence
Target Sequence 
>C. ELEGANS:177154_AT
aaggcggaggatattcgtacaaaagtgctcgattcaatgatctaccgactcgatgaacgt
gcaaatatggttatcacggagagcccaattcccgaatggcgtgcttatacacaatggatt
ctcattgctgcgttgcttgtaattttttgtattcttcgttatggcgtcgagactgttgaa
ctgcatccaactgcaaagaaggatcaagatcctgtgattgagtac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGCGGAGGATATTCGTACAAAAG 342 1 49 Antisense
GCTCGATTCAATGATCTACCGACTC 319 299 75 Antisense
TCTACCGACTCGATGAACGTGCAAA 701 611 89 Antisense
TATCACGGAGAGCCCAATTCCCGAA 562 659 120 Antisense
AATTCCCGAATGGCGTGCTTATACA 34 179 135 Antisense
GTGCTTATACACAATGGATTCTCAT 543 479 149 Antisense
GCGTTGCTTGTAATTTTTTGTATTC 176 291 178 Antisense
GTATTCTTCGTTATGGCGTCGAGAC 689 451 197 Antisense
TGGCGTCGAGACTGTTGAACTGCAT 206 545 210 Antisense
GACTGTTGAACTGCATCCAACTGCA 580 375 219 Antisense
GGATCAAGATCCTGTGATTGAGTAC 354 511 249 Antisense
Sequence Source Affymetrix Proprietary Database