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[177148_s_at]

Description T01A4.1B /REP_DB=WormBase Gene ID /WP=CE25977 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=guanylyl cyclase
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 03      4grp_high_8_8 > 32      6grp_highest_nonorm > 80      6grp_total > 34      4grp_total > 47      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 258
1 2 3 4 range
A 328.9
(3)
P 		105.2
(-2.2)
P 		70.5
(-3)
P 		98.4
(-2.4)
A 		258
B 271.4
(3)
P 		136.1
(-1.4)
P 		88.6
(-3)
P 		131.1
(-1.6)
P 		183
C 315.8
(3)
P 		126.1
(-2.9)
P 		139.5
(-2.5)
P 		124.4
(-3)
P 		191
avr 305.4
(3) 		122.5
(-2.3) 		99.5
(-3) 		118
(-2.5) 		206

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 177
1 2 3 4 5 6 range
A 188.1
(1.2)
P 		166.9
(0.1)
P 		223.6
(3)
P 		141.2
(-1.3)
P 		133
(-1.7)
P 		108
(-3)
P 		116
B 246.3
(2.8)
P 		252.2
(3)
P 		191.4
(0.6)
P 		99.3
(-3)
P 		117.4
(-2.3)
P 		100.1
(-3)
P 		153
C 274.5
(3)
P 		214.6
(1)
P 		195.6
(0.3)
P 		118.2
(-2.3)
P 		97.9
(-3)
P 		123
(-2.1)
P 		177
avr 236.3
(3) 		211.2
(1.8) 		203.5
(1.4) 		119.6
(-2.6) 		116.1
(-2.7) 		110.4
(-3) 		126
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 119.8
(3)
P 		22.8
(-2.5)
P 		18.4
(-2.8)
P 		14.4
(-3)
A 		105
B 86.6
(3)
P 		33.6
(-1.8)
P 		20.5
(-3)
P 		27.9
(-2.3)
P 		66
C 104.6
(3)
P 		36.3
(-1.9)
P 		48.6
(-1)
P 		21
(-3)
P 		84
avr 103.7
(3) 		30.9
(-2.3) 		29.2
(-2.4) 		21.1
(-3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 34.7
(-1.5)
P 		59.4
(3)
P 		48.7
(1.1)
P 		35.8
(-1.3)
P 		40.1
(-0.5)
P 		26.3
(-3)
P 		33
B 85.7
(3)
P 		84
(2.8)
P 		46.5
(-1)
P 		26.3
(-3)
P 		47.7
(-0.8)
P 		37.3
(-1.9)
P 		59
C 101.7
(3)
P 		65.5
(0.2)
P 		49.8
(-1)
P 		23.8
(-3)
P 		31.7
(-2.4)
P 		41.2
(-1.7)
P 		78
avr 74
(3) 		69.6
(2.4) 		48.3
(-0.4) 		28.6
(-3) 		39.8
(-1.5) 		34.9
(-2.2) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177148_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.744
Sequence Type Exemplar sequence
Representative Public ID T01A4.1B NCBI
Target Description T01A4.1B /REP_DB=WormBase Gene ID /WP=CE25977 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=guanylyl cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026429(11), NM_058978(11), NM_058979(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026429 NCBI Caenorhabditis elegans T01A4.1c (T01A4.1) mRNA, complete cds. 11/11 None
NM_058978 NCBI Caenorhabditis elegans T01A4.1a (T01A4.1) mRNA, complete cds. 11/11 A 
NM_058979 NCBI Caenorhabditis elegans T01A4.1b (T01A4.1) mRNA, complete cds. 11/11 None
SNAP00000012121 ENSEMBL cdna:SNAP chromosome:CEL140:I:4503271:4508208:1 11/11 None
GENEFINDER00000012139 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4503271:4508208:1 11/11 None
T01A4.1c ENSEMBL cdna:known chromosome:CEL140:I:4491702:4508932:1 gene:T01A4.1 11/11 None
T01A4.1a ENSEMBL cdna:known chromosome:CEL140:I:4495393:4508208:1 gene:T01A4.1 11/11 None
T01A4.1b ENSEMBL cdna:known chromosome:CEL140:I:4502610:4507720:1 gene:T01A4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4502609-4507720(+) 97.81 100.0
Public Domain and Genome References
Ensembl T01A4.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO61423 Hypothetical protein T01A4.1c [Caenorhabditis elegans] ref|NP_001021600.1| T01A4.1c [Caenorhabditis elegans] 0.0
blast AAL77521 guanylyl cyclase [Caenorhabditis elegans] 0.0
blast AAO61422 Hypothetical protein T01A4.1a [Caenorhabditis elegans] ref|NP_491379.3| T01A4.1a [Caenorhabditis elegans] 0.0
blast CAE68894 Hypothetical protein CBG14865 [Caenorhabditis briggsae] 0.0
blast AAK71394 Hypothetical protein T01A4.1b [Caenorhabditis elegans] ref|NP_491380.1| T01A4.1b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-19
Pfam IPR000719 EMBL-EBI Protein kinase 2.6E-9
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.4E-97
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 5.4E-35
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-19
Pfam IPR000719 EMBL-EBI Protein kinase 2.6E-9
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.4E-97
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 1.2E-51
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-19
Pfam IPR000719 EMBL-EBI Protein kinase 2.6E-9
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.3E-77
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-19
Pfam IPR000719 EMBL-EBI Protein kinase 2.6E-9
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.4E-97
Pfam IPR000719 EMBL-EBI Protein kinase 0.0034
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-9
Pfam IPR000719 EMBL-EBI Protein kinase 2.6E-9
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.3E-77
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.5E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021600.1 1 514-536
NP_491379.3 1 469-491
T01A4.1c 1 514-536
T01A4.1a 1 469-491
Sequence
Target Sequence 
>C. ELEGANS:177148_S_AT
aatcgccgacactttaattgctggacgagctgttcaagctgaatcgtatgattgtgtaac
tatctacttttctgatattgtcggattcacatcactatcatcacaatcaacaccaatgca
agtggtcacattactcaatgatttatatttggcatttgatggagttgtagacaatttcaa
agtatataaagttgagactattggtgatgcatacatggtagtatctggattaccagaacg
acgagatgatcatgctaatcaaatagctcaaatgtcactatcattattgcataaagttaa
gaattttgtgattcgacatcgacctcatgaacagctcaaacttcgtatcggaatgcattc
aggatccgttgtggcaggagtcgtaggttcaaaaatgcctcgatactgtcttttcggaga
tactgtgaacacttcgagtcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCGCCGACACTTTAATTGCTGGA 195 169 1329 Antisense
AATTGCTGGACGAGCTGTTCAAGCT 408 177 1344 Antisense
GATTGTGTAACTATCTACTTTTCTG 230 435 1378 Antisense
CTACTTTTCTGATATTGTCGGATTC 354 225 1392 Antisense
GTCGGATTCACATCACTATCATCAC 582 471 1408 Antisense
TCGACATCGACCTCATGAACAGCTC 316 601 1641 Antisense
GAACAGCTCAAACTTCGTATCGGAA 71 351 1657 Antisense
GAATGCATTCAGGATCCGTTGTGGC 299 331 1679 Antisense
CGTTGTGGCAGGAGTCGTAGGTTCA 325 237 1695 Antisense
AATGCCTCGATACTGTCTTTTCGGA 435 167 1722 Antisense
GATACTGTGAACACTTCGAGTCGAA 631 423 1747 Antisense
Sequence Source Affymetrix Proprietary Database