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[177147_at]

Description AC3.4 /REP_DB=WormBase Gene ID /WP=CE05134 /TR=Q17401 /GB=CAA94868.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 84      4grp_total > 04      6grp_cgc > 77      6grp_cgc_sub > 04_05_06      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 12.4
(-3)
A 		13.6
(-2.9)
A 		15.9
(-2.7)
A 		87.2
(3)
P 		75
B 43.1
(-0.9)
A 		14.8
(-3)
A 		18.9
(-2.7)
A 		95.4
(3)
P 		81
C 34.9
(-1.1)
A 		11.6
(-2.6)
A 		4.8
(-3)
A 		98.2
(3)
P 		93
avr 30.1
(-1.7) 		13.3
(-3) 		13.2
(-3) 		93.6
(3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 148
1 2 3 4 5 6 range
A 32.9
(-3)
A 		51.8
(-2.1)
A 		125.2
(1.4)
P 		141
(2.2)
P 		131.9
(1.7)
P 		158.4
(3)
P 		126
B 23.9
(-3)
A 		41.8
(-2.1)
A 		68
(-0.8)
P 		135.2
(2.6)
P 		142.1
(3)
P 		89.7
(0.3)
P 		118
C 10.9
(-3)
A 		108.9
(1.4)
P 		58.1
(-0.9)
A 		108.7
(1.4)
P 		145.7
(3)
P 		92.4
(0.6)
P 		135
avr 22.6
(-3) 		67.5
(-0.7) 		83.8
(0.1) 		128.3
(2.4) 		139.9
(3) 		113.5
(1.6) 		117
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 4.5
(-2.1)
A 		3
(-3)
A 		4.1
(-2.3)
A 		12.8
(3)
P 		10
B 13.8
(0.7)
A 		3.7
(-3)
A 		4.4
(-2.7)
A 		20.3
(3)
P 		17
C 11.6
(1)
A 		3.3
(-2.4)
A 		1.7
(-3)
A 		16.6
(3)
P 		15
avr 10
(0) 		3.3
(-3) 		3.4
(-3) 		16.6
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 6.1
(-3)
A 		18.4
(-0.8)
A 		27.3
(0.8)
P 		35.8
(2.3)
P 		39.8
(3)
P 		38.6
(2.8)
P 		34
B 8.3
(-3)
A 		13.9
(-2.3)
A 		16.5
(-2)
P 		35.8
(0.3)
P 		57.7
(3)
P 		33.4
(0)
P 		49
C 4
(-3)
A 		33.2
(1.1)
P 		14.8
(-1.5)
A 		21.9
(-0.5)
P 		47.1
(3)
P 		30.9
(0.7)
P 		43
avr 6.1
(-3) 		21.8
(-0.8) 		19.5
(-1.1) 		31.2
(0.6) 		48.2
(3) 		34.3
(1) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177147_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11471
Sequence Type Exemplar sequence
Representative Public ID AC3.4 NCBI
Target Description AC3.4 /REP_DB=WormBase Gene ID /WP=CE05134 /TR=Q17401 /GB=CAA94868.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026168(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026168 NCBI Caenorhabditis elegans AC3.10 (AC3.10) mRNA, complete cds. 11/11 None
SNAP00000028836 ENSEMBL cdna:SNAP chromosome:CEL140:V:10387114:10388266:1 11/11 None
GENEFINDER00000028849 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10386930:10388276:1 11/11 None
AC3.10 ENSEMBL cdna:known chromosome:CEL140:V:10386930:10388266:1 gene:AC3.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10385679-10388266(+) 98.17 100.0
chrV:10383011-10383886(-) 46.64 48.36
Public Domain and Genome References
Gene Title
Ensembl AC3.10
Entrez Gene 3565514 Entrez gene
SwissProt Q5FC64 EMBL-EBI
WormBase CE37870 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
46872 metal ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAI46554 Hypothetical protein AC3.10 [Caenorhabditis elegans] ref|NP_001021339.1| AC3.10 [Caenorhabditis elegans] 0.0
blast CAI46554 Hypothetical protein AC3.10 [Caenorhabditis elegans] ref|NP_001021339.1| AC3.10 [Caenorhabditis elegans] 1.0E-177
blast CAE64826 Hypothetical protein CBG09622 [Caenorhabditis briggsae] 1.0E-171
blast CAE64826 Hypothetical protein CBG09622 [Caenorhabditis briggsae] 1.0E-164
blast CAE64826 Hypothetical protein CBG09622 [Caenorhabditis briggsae] 1.0E-156
blast EAA10350 ENSANGP00000022226 [Anopheles gambiae str. PEST] ref|XP_315027.2| ENSANGP00000022226 [Anopheles gambiae str. PEST] 2.0E-38
blast EAA10350 ENSANGP00000022226 [Anopheles gambiae str. PEST] ref|XP_315027.2| ENSANGP00000022226 [Anopheles gambiae str. PEST] 3.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR001594 EMBL-EBI Zn-finger, DHHC type 4.7E-36
Pfam IPR001594 EMBL-EBI Zn-finger, DHHC type 4.7E-36
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021339.1 4 21-43,58-80,198-215,239-261
AC3.10 4 21-43,58-80,198-215,239-261
SNAP00000028836 3 13-35,153-170,194-216
GENEFINDER00000028849 4 21-43,58-80,198-215,239-261
Sequence
Target Sequence 
>C. ELEGANS:177147_AT
atatcaaaccggatcgtgcccgacattgtagctcatgtgggaagtgttgcattaagtatg
atcatcactgtccatggataaacatgtgtgttacacacgtcaattacaagtactttctac
tctacattatctacaccagttttcttgtttattggtatttgctcacttcactagaaggtg
ctgttcgatattttatcaatcaacaatggaccgatgaattgggaaagtttttgttctact
tgttcagttttattgtcggcggagtgtttggttactatccactcggtgaacttatcatat
tccattatcaattgatctcattgaacgagacaactgtcgaacaaacaaaaccagctcttc
taagatttgataatgcagctgactacaatatggggaaatataataattttcaatcagtgt
ttggatggggattatggctgtgtccaattgattccagcacccaggatggtctgcatttcg
acataagatacgtcaacacccagcaacggaatcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATCAAACCGGATCGTGCCCGACA 160 11 1241 Antisense
GTGCCCGACATTGTAGCTCATGTGG 51 479 1256 Antisense
TACTTTCTACTCTACATTATCTACA 384 645 1351 Antisense
TTATCTACACCAGTTTTCTTGTTTA 687 679 1367 Antisense
ATTGGTATTTGCTCACTTCACTAGA 396 3 1391 Antisense
ACTTGTTCAGTTTTATTGTCGGCGG 223 103 1478 Antisense
GTCGGCGGAGTGTTTGGTTACTATC 284 439 1495 Antisense
GGTTACTATCCACTCGGTGAACTTA 293 503 1510 Antisense
TGTGTCCAATTGATTCCAGCACCCA 692 555 1679 Antisense
GCACCCAGGATGGTCTGCATTTCGA 644 313 1697 Antisense
GTCAACACCCAGCAACGGAATCGAT 196 463 1732 Antisense
Sequence Source Affymetrix Proprietary Database