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[177144_at]

Description Y45G5AM.K /REP_DB=WormBase Gene ID /WP=CE26408 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23368.1
Cluster 6grp_total > 22      4grp_total > 58      6grp_cgc > 61      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 262
1 2 3 4 range
A 148.4
(-0.2)
A 		227
(3)
A 		219.2
(2.7)
A 		81.6
(-3)
A 		145
B 166
(0)
A 		195.1
(0.7)
A 		295.7
(3)
A 		33.8
(-3)
A 		262
C 152.4
(-0.2)
A 		261.8
(3)
A 		251.7
(2.7)
P 		57.2
(-3)
A 		205
avr 155.6
(-0) 		228
(2.2) 		255.5
(3) 		57.5
(-3) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 346
1 2 3 4 5 6 range
A 389.5
(1.7)
A 		454.5
(3)
P 		267
(-0.6)
A 		310.9
(0.2)
P 		292.7
(-0.1)
A 		144.8
(-3)
A 		310
B 271.2
(1.5)
A 		325.1
(3)
P 		294.2
(2.1)
P 		302.8
(2.4)
M 		248.8
(0.9)
A 		108.1
(-3)
A 		217
C 356.5
(1.6)
P 		350.3
(1.5)
P 		430.6
(3)
P 		258.5
(-0.2)
A 		215.7
(-1)
M 		110.9
(-3)
A 		320
avr 339.1
(2.1) 		376.6
(3) 		330.6
(1.9) 		290.7
(1) 		252.4
(0.1) 		121.3
(-3) 		255
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 54
(2.6)
A 		49.3
(1.9)
A 		57.3
(3)
A 		11.9
(-3)
A 		45
B 52.9
(1.5)
A 		48.2
(1)
A 		68.5
(3)
A 		7.2
(-3)
A 		61
C 50.5
(0.1)
A 		75.3
(2)
A 		87.7
(3)
P 		9.7
(-3)
A 		78
avr 52.5
(1.2) 		57.6
(1.7) 		71.2
(3) 		9.6
(-3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 71.8
(-1.3)
A 		161.6
(3)
P 		58.1
(-1.9)
A 		78.8
(-0.9)
P 		88.3
(-0.5)
A 		35.3
(-3)
A 		126
B 94.4
(1.8)
A 		108.2
(3)
P 		71.4
(-0.3)
P 		80.2
(0.5)
M 		101.1
(2.4)
A 		40.3
(-3)
A 		68
C 132.1
(3)
P 		106.9
(1.4)
P 		109.6
(1.6)
P 		52
(-2.1)
A 		69.8
(-0.9)
M 		37.1
(-3)
A 		95
avr 99.4
(1.2) 		125.6
(3) 		79.7
(-0.1) 		70.3
(-0.8) 		86.4
(0.3) 		37.6
(-3) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177144_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19543
Sequence Type Exemplar sequence
Representative Public ID Y45G5AM.K NCBI
Target Description Y45G5AM.K /REP_DB=WormBase Gene ID /WP=CE26408 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23368.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029071(11), NM_001029072(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029071 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-114) (nhr-114) mRNA, complete cds. 11/11 None
NM_001029072 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-114) (nhr-114) mRNA, complete cds. 11/11 None
Y45G5AM.1a.2 ENSEMBL cdna:known chromosome:CEL140:V:4186999:4189820:1 gene:Y45G5AM.1 11/11 None
Y45G5AM.1a.1 ENSEMBL cdna:known chromosome:CEL140:V:4187017:4189820:1 gene:Y45G5AM.1 11/11 None
Y45G5AM.1b ENSEMBL cdna:known chromosome:CEL140:V:4187017:4189824:1 gene:Y45G5AM.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4187022-4188350(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y45G5AM.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024242 Nuclear Hormone Receptor family member (nhr-114) [Caenorhabditis elegans] gb|AAG23368.3| Nuclear hormone receptor family protein 114, isoform a [Caenorhabditis elegans] gb|AAO27833.1| nuclear receptor protein [Caenorhabditis elegans] 0.0
blast AAO39202 nuclear receptor NHR-114 [Caenorhabditis elegans] 0.0
blast CAE58326 Hypothetical protein CBG01439 [Caenorhabditis briggsae] 0.0
blast NP_001024243 Nuclear Hormone Receptor family member (nhr-114) [Caenorhabditis elegans] gb|AAO39201.1| nuclear receptor NHR-114 [Caenorhabditis elegans] gb|AAQ91893.1| Nuclear hormone receptor family protein 114, isoform b [Caenorhabditis elegans] 1.0E-169
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 3.4E-32
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 5.1E-46
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 6.6E-13
Sequence
Target Sequence 
>C. ELEGANS:177144_AT
aaccgtgaccctatcggatacacaaaacgtaccaggcgtccaaaaaaagagctgaagact
acctcggactgttcaagtgacgaaggagcaagcaccccaccatcagtgtctccattacag
ctatcgcctccaccaatctcgccactccttttccaagctgcaccactgaaacctcgtcgc
tgcatcctacaaactcttgccgaacgcgaaaagtgtgcaaatgatctccgactctccgaa
tatcttccaatccgatctctccacgaggctctatgctcaaaggctctgctgaacgacacg
gcttttttggaaaagtggggacagccgagtgagagacatcagatcttcgatctacgattc
gtcaatcacgacgactaccactattggcacgagagagactggttcttgttgacagagtat
gccaaaacattcgatgtttttgaggcgctcgattatcaagataaggccgaacttgttcgc
catgccgcaataacagtcccagtcctggttcaagtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCGTGACCCTATCGGATACACAA 609 145 274 Antisense
GACTACCTCGGACTGTTCAAGTGAC 367 371 330 Antisense
TCAGTGTCTCCATTACAGCTATCGC 427 623 376 Antisense
TTTCCAAGCTGCACCACTGAAACCT 26 669 423 Antisense
AGTGTGCAAATGATCTCCGACTCTC 60 59 485 Antisense
TCCGAATATCTTCCAATCCGATCTC 559 595 508 Antisense
AGGCTCTATGCTCAAAGGCTCTGCT 192 55 539 Antisense
CTCTGCTGAACGACACGGCTTTTTT 573 229 557 Antisense
ATCACGACGACTACCACTATTGGCA 261 29 638 Antisense
CTTGTTCGCCATGCCGCAATAACAG 703 217 745 Antisense
AACAGTCCCAGTCCTGGTTCAAGTC 699 135 765 Antisense
Sequence Source Affymetrix Proprietary Database