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[177124_at]

Description Y19D10B.B /REP_DB=WormBase Gene ID /WP=CE25225 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59425.1
Cluster 6grp_total > 64      4grp_total > 24      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 2.8
(-3)
A 		11.3
(-1.3)
A 		30.6
(2.6)
A 		32.6
(3)
A 		30
B 1.6
(-3)
A 		3.4
(-2.5)
A 		22.2
(3)
A 		6.2
(-1.7)
A 		21
C 0.7
(-3)
A 		11.8
(-1.9)
A 		7
(-2.3)
A 		58.8
(3)
M 		58
avr 1.7
(-3) 		8.8
(-1.6) 		19.9
(0.5) 		32.5
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 2
(-3)
A 		3.1
(-2.8)
A 		21
(0.9)
A 		9.7
(-1.4)
A 		10.3
(-1.3)
A 		31.1
(3)
A 		29
B 0.6
(-3)
A 		1.4
(-2.8)
A 		28.8
(3)
P 		23.8
(1.9)
P 		3.9
(-2.3)
A 		21.3
(1.4)
A 		28
C 0.3
(-3)
A 		21.5
(3)
A 		10.4
(-0.1)
A 		16.5
(1.6)
A 		14.4
(1)
A 		15.8
(1.4)
A 		21
avr 1
(-3) 		8.7
(-0.9) 		20.1
(2.3) 		16.7
(1.3) 		9.5
(-0.6) 		22.7
(3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 1
(-3)
A 		2.5
(-1.7)
A 		8
(3)
A 		4.8
(0.3)
A 		7
B 0.5
(-3)
A 		0.9
(-2.5)
A 		5.1
(3)
A 		1.3
(-2)
A 		5
C 0.2
(-3)
A 		3.4
(-1)
A 		2.5
(-1.6)
A 		9.9
(3)
M 		10
avr 0.6
(-3) 		2.3
(-0.8) 		5.2
(2.9) 		5.3
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.4
(-3)
A 		1.1
(-2.4)
A 		4.6
(0.5)
A 		2.5
(-1.3)
A 		3.1
(-0.8)
A 		7.6
(3)
A 		7
B 0.2
(-3)
A 		0.5
(-2.8)
A 		7
(2.3)
P 		6.3
(1.8)
P 		1.6
(-1.9)
A 		7.9
(3)
A 		8
C 0.1
(-3)
A 		6.6
(3)
A 		2.6
(-0.7)
A 		3.3
(-0)
A 		4.7
(1.2)
A 		5.3
(1.8)
A 		7
avr 0.2
(-3) 		2.7
(-0.8) 		4.7
(1) 		4
(0.4) 		3.1
(-0.4) 		6.9
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177124_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19468
Sequence Type Exemplar sequence
Representative Public ID Y19D10B.B NCBI
Target Description Y19D10B.B /REP_DB=WormBase Gene ID /WP=CE25225 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59425.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071235(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071235 NCBI Caenorhabditis elegans Y19D10B.6 (Y19D10B.6) mRNA, complete cds. 11/11 None
SNAP00000023997 ENSEMBL cdna:SNAP chromosome:CEL140:V:2318521:2320514:-1 11/11 None
GENEFINDER00000024001 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2318521:2322367:-1 11/11 None
Y19D10B.6 ENSEMBL cdna:known chromosome:CEL140:V:2318353:2320880:-1 gene:Y19D10B.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2318520-2320880(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y19D10B.6
Entrez Gene 189497 Entrez gene
SwissProt Q9N566 EMBL-EBI
WormBase CE25225 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF59425 Hypothetical protein Y19D10B.6 [Caenorhabditis elegans] ref|NP_503636.1| Y19D10B.6 [Caenorhabditis elegans] 0.0
blast NP_503898 T28A11.17 [Caenorhabditis elegans] gb|AAC48126.2| Hypothetical protein T28A11.17 [Caenorhabditis elegans] 0.0
blast AAB37589 Hypothetical protein T20D4.8 [Caenorhabditis elegans] ref|NP_503953.1| T20D4.8 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 6.7E-84
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 6.7E-84
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 6.7E-84
Sequence
Target Sequence 
>C. ELEGANS:177124_AT
cccgaagtgatttacaacaacctatgttagcgatagccgacaccgtttccgtgcttgccc
atgagcttatgcactttgtctatccaagcggaacagagttactgactcctaatgttacaa
aagcagcaaaaaagtgtacacaagatgaagtgaagaggatgggtgactctgatgtcgtga
agccaatagatggatggtttagtaaaaaagtcactcatgaggatcttgcaaatataatgg
gattaaggatggttatgaagatggtggcaaggaaatcggcaagtgaagagcagctgaaaa
atgcgcttgaaacacttttgagtggattatgcatccaaggcaaaccaaaaaactcaattt
taccacaccatcatccatttgaaatatccatcaacaccgcagttcgccagtatccattgt
tcagctcactctatggatgtcgccttggtgatcgaatgtttgctgaagcggaacaatttt
gcaaaccacttggaggcgaagtgaatttggaggactataaaatcaagaatgatactgaag
atattggtggattctttacttacattatggataccgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCGAAGTGATTTACAACAACCTAT 598 259 884 Antisense
ACAACAACCTATGTTAGCGATAGCC 158 111 897 Antisense
TGGTGGCAAGGAAATCGGCAAGTGA 445 553 1145 Antisense
AATGCGCTTGAAACACTTTTGAGTG 19 167 1183 Antisense
ACACTTTTGAGTGGATTATGCATCC 65 111 1195 Antisense
AAACTCAATTTTACCACACCATCAT 130 129 1233 Antisense
TTTACCACACCATCATCCATTTGAA 385 673 1242 Antisense
GAAGCGGAACAATTTTGCAAACCAC 176 343 1348 Antisense
TTTGCAAACCACTTGGAGGCGAAGT 245 563 1361 Antisense
TTGGTGGATTCTTTACTTACATTAT 361 701 1427 Antisense
CTTTACTTACATTATGGATACCGCA 248 223 1437 Antisense
Sequence Source Affymetrix Proprietary Database