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[177120_s_at]

Description CC4.2 /REP_DB=WormBase Gene ID /WP=CE15741 /TR=O17723 /GB=CAB04013.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_highest_8_8 > 47      4grp_high_8_8 > 03      6grp_highest_nonorm > 84      6grp_total > 48      4grp_total > 13      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1641
1 2 3 4 range
A 1125.4
(0.1)
P 		639.6
(-2)
P 		395.1
(-3)
P 		1796.1
(3)
P 		1401
B 1196.9
(-0.1)
P 		482.6
(-2.8)
P 		421.8
(-3)
P 		2035.6
(3)
P 		1614
C 1193.2
(0)
P 		541
(-2.6)
P 		431
(-3)
P 		1947.2
(3)
P 		1516
avr 1171.8
(0) 		554.4
(-2.5) 		416
(-3) 		1926.3
(3) 		1510

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 494
1 2 3 4 5 6 range
A 550.4
(-1.1)
P 		784
(3)
P 		439.5
(-3)
P 		660.1
(0.8)
P 		480.6
(-2.3)
P 		727
(2)
P 		345
B 471.6
(-1.8)
P 		550.6
(0.3)
P 		478.1
(-1.6)
P 		426.3
(-3)
P 		584.5
(1.2)
P 		654.9
(3)
P 		229
C 920.3
(3)
P 		556.5
(-2.1)
P 		547.9
(-2.3)
P 		496.4
(-3)
P 		666.2
(-0.6)
P 		864.7
(2.2)
P 		424
avr 647.4
(0.7) 		630.4
(0.3) 		488.5
(-3) 		527.6
(-2.1) 		577.1
(-1) 		748.9
(3) 		260
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 336
1 2 3 4 range
A 409.8
(3)
P 		138.8
(-2.3)
P 		103.3
(-3)
P 		262.9
(0.1)
P 		307
B 381.7
(2.1)
P 		119.2
(-2.6)
P 		97.7
(-3)
P 		433.8
(3)
P 		336
C 395
(3)
P 		155.5
(-2.9)
P 		150.2
(-3)
P 		328.3
(1.4)
P 		245
avr 395.5
(3) 		137.8
(-2.6) 		117.1
(-3) 		341.7
(1.8) 		278

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 101.5
(-2.8)
P 		278.8
(3)
P 		95.7
(-3)
P 		167.4
(-0.7)
P 		145
(-1.4)
P 		177
(-0.3)
P 		183
B 164.1
(-0.7)
P 		183.3
(0.2)
P 		116.1
(-2.9)
P 		112.9
(-3)
P 		237.5
(2.7)
P 		244.2
(3)
P 		131
C 341.1
(3)
P 		169.8
(-1.3)
P 		139.4
(-2)
P 		99.8
(-3)
P 		215.6
(-0.1)
P 		289.5
(1.7)
P 		241
avr 202.2
(1.3) 		210.6
(1.7) 		117.1
(-3) 		126.7
(-2.5) 		199.4
(1.1) 		236.9
(3) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177120_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.575
Sequence Type Exemplar sequence
Representative Public ID CC4.2 NCBI
Target Description CC4.2 /REP_DB=WormBase Gene ID /WP=CE15741 /TR=O17723 /GB=CAB04013.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060877(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060877 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-15) (nlp-15) mRNA, complete cds. 11/11 None
SNAP00000007717 ENSEMBL cdna:SNAP chromosome:CEL140:I:13002347:13012258:1 11/11 None
GENEFINDER00000007720 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13002347:13012258:1 11/11 None
CC4.2 ENSEMBL cdna:known chromosome:CEL140:I:12996353:13003591:1 gene:CC4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13002277-13003186(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-15
Ensembl CC4.2
Entrez Gene 173174 Entrez gene
SwissProt Q7JK63 EMBL-EBI
WormBase CE36118 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04014 Hypothetical protein CC4.3 [Caenorhabditis elegans] ref|NP_493279.1| Suppressor of Mec and Unc defects family member (smu-1) [Caenorhabditis elegans] gb|AAK00353.1| SMU-1 [Caenorhabditis elegans] 0.0
blast CAE63459 Hypothetical protein CBG07923 [Caenorhabditis briggsae] 0.0
blast AAH45945 Smu-1 suppressor of mec-8 and unc-52 homolog [Danio rerio] ref|NP_956493.1| smu-1 suppressor of mec-8 and unc-52 homolog [Danio rerio] 0.0
blast AAH45945 Smu-1 suppressor of mec-8 and unc-52 homolog [Danio rerio] ref|NP_956493.1| smu-1 suppressor of mec-8 and unc-52 homolog [Danio rerio] 1.0E-174
blast CAE54928 Hypothetical protein CC4.2 [Caenorhabditis elegans] emb|CAB04013.2| Hypothetical protein CC4.2 [Caenorhabditis elegans] ref|NP_493278.2| Neuropeptide-Like Protein family member (nlp-15) [Caenorhabditis elegans] 4.0E-72
blast T20275 hypothetical protein CC4.2 - Caenorhabditis elegans 4.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.7E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.2E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.4E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.7E-12
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.7E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.2E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.6E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.7E-12
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.11
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493278.2 1 7-29
CC4.2 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:177120_S_AT
agtggaaagtgcagctgtccgactacgtccagttggctcattgttcttcctgaatcgacc
tcacgagaaaagagcattcgattcgttagctggaagtggttttgataatggattcaacaa
acgagcattcgacagtcttgctggttcaggtttcggagcattcaataaaagagcatttga
ttcattggcgggaagtggcttcggtgcgttcaacaaacgcgcttttgactcgctagccgg
atccggcttcagcggttttgacaaaagagctttcgattcactggctggacaaggttttac
tggatttgagaaacgcgcctttgatacagtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTGGAAAGTGCAGCTGTCCGACTA 174 59 21 Antisense
GACTACGTCCAGTTGGCTCATTGTT 366 371 41 Antisense
TCATTGTTCTTCCTGAATCGACCTC 386 619 58 Antisense
ACGAGCATTCGACAGTCTTGCTGGT 401 93 141 Antisense
GTCTTGCTGGTTCAGGTTTCGGAGC 102 447 155 Antisense
ATTGGCGGGAAGTGGCTTCGGTGCG 627 1 204 Antisense
CAACAAACGCGCTTTTGACTCGCTA 325 189 231 Antisense
TGACTCGCTAGCCGGATCCGGCTTC 195 571 246 Antisense
GATCCGGCTTCAGCGGTTTTGACAA 361 415 260 Antisense
AGAGCTTTCGATTCACTGGCTGGAC 38 71 286 Antisense
TGAGAAACGCGCCTTTGATACAGTG 131 569 327 Antisense
Sequence Source Affymetrix Proprietary Database