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[177116_at]

Description M7.12 /REP_DB=WormBase Gene ID /WP=CE12222 /TR=Q21596 /GB=CAA92749.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 16      6grp_total > 15      4grp_total > 47      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 499
1 2 3 4 range
A 504.8
(3)
P 		142.7
(-1.4)
P 		67.2
(-2.3)
P 		5.6
(-3)
A 		499
B 469.5
(3)
P 		100.7
(-1.8)
P 		60.1
(-2.3)
P 		7.5
(-3)
A 		462
C 462.3
(3)
P 		96.7
(-2.4)
P 		106.2
(-2.2)
P 		52.4
(-3)
A 		410
avr 478.9
(3) 		113.4
(-1.8) 		77.8
(-2.3) 		21.8
(-3) 		457

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 318
1 2 3 4 5 6 range
A 271.9
(3)
P 		167
(0.5)
P 		129.4
(-0.3)
P 		98.6
(-1.1)
P 		53.3
(-2.1)
P 		16.4
(-3)
A 		256
B 334.4
(3)
P 		185
(-0.2)
P 		167.9
(-0.5)
P 		70.7
(-2.6)
P 		88.2
(-2.2)
P 		52.9
(-3)
P 		282
C 256.1
(3)
P 		230.8
(2.3)
P 		103.6
(-1.2)
P 		63.1
(-2.3)
P 		36.6
(-3)
P 		60.7
(-2.3)
P 		220
avr 287.5
(3) 		194.3
(0.7) 		133.6
(-0.8) 		77.5
(-2.2) 		59.4
(-2.6) 		43.3
(-3) 		244
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 183.8
(3)
P 		31
(-2)
P 		17.6
(-2.4)
P 		0.8
(-3)
A 		183
B 149.7
(3)
P 		24.9
(-2.1)
P 		13.9
(-2.5)
P 		1.6
(-3)
A 		148
C 153
(3)
P 		27.8
(-2.2)
P 		37
(-1.8)
P 		8.8
(-3)
A 		144
avr 162.2
(3) 		27.9
(-2.1) 		22.8
(-2.3) 		3.7
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 50.1
(2)
P 		59.4
(3)
P 		28.2
(-0.4)
P 		25
(-0.7)
P 		16.1
(-1.7)
P 		4
(-3)
A 		55
B 116.4
(3)
P 		61.6
(-0.4)
P 		40.8
(-1.6)
P 		18.7
(-3)
P 		35.8
(-1.9)
P 		19.7
(-2.9)
P 		98
C 94.9
(3)
P 		70.4
(1.2)
P 		26.4
(-1.9)
P 		12.7
(-2.9)
P 		11.8
(-3)
P 		20.3
(-2.4)
P 		83
avr 87.1
(3) 		63.8
(1.1) 		31.8
(-1.6) 		18.8
(-2.7) 		21.2
(-2.5) 		14.7
(-3) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177116_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8872
Sequence Type Exemplar sequence
Representative Public ID M7.12 NCBI
Target Description M7.12 /REP_DB=WormBase Gene ID /WP=CE12222 /TR=Q21596 /GB=CAA92749.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069669(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069669 NCBI Caenorhabditis elegans M7.12 (M7.12) mRNA, complete cds. 11/11 None
SNAP00000010386 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11096750:11097812:-1 11/11 None
GENEFINDER00000010395 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11096750:11100343:-1 11/11 None
M7.12 ENSEMBL cdna:known chromosome:CEL140:IV:11096750:11097812:-1 gene:M7.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11096755-11097818(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M7.12
Entrez Gene 187458 Entrez gene
SwissProt Q21596 EMBL-EBI
WormBase CE37394 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92751 Hypothetical protein M7.13 [Caenorhabditis elegans] ref|NP_502071.1| Seven TM Receptor family member (str-3) [Caenorhabditis elegans] 1.0E-175
blast CAA92749 Hypothetical protein M7.12 [Caenorhabditis elegans] ref|NP_502070.2| M7.12 [Caenorhabditis elegans] 1.0E-117
blast NP_504817 Seven TM Receptor family member (str-204) [Caenorhabditis elegans] gb|AAC48232.1| Seven tm receptor protein 204 [Caenorhabditis elegans] 2.0E-73
blast CAA92743 Hypothetical protein M7.8 [Caenorhabditis elegans] ref|NP_502069.1| M7.8 [Caenorhabditis elegans] 1.0E-62
blast CAE61997 Hypothetical protein CBG06005 [Caenorhabditis briggsae] 2.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 5.1E-32
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000010395 6 15-37,58-80,95-117,137-159,207-229,253-275
Sequence
Target Sequence 
>C. ELEGANS:177116_AT
gaaccattgagcaaggccgctggaatgactgaatctgacatacaatcatgttttgatgga
gtatttgaaagagtattgaagaatgaagtttccattcttgcaagatcaaaacagtccgat
gaaattgttggatgtatgctgaattctgtttggaagagaaatgatccaaaaaagaatgaa
gatgaggcagaggattttgagttcggaggggatcgaaaaggcgtaatgacaattggagaa
atattaaatgaacttcatgaatccttctggaaactacgtccagatcagcatacagtcttg
cattttgaaatttcgtctgttaataagaatcatcagagacaaggattggcatcaaaattc
atgaattggacggagaaaaaggaacttttgaaggtaattgaagcaagcagcattgtcgct
gaggcttcatcattggctaatcaaattctgttaagcaaaagaggatatgagactgttgct
tcaactctcttgagttctcgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCATTGAGCAAGGCCGCTGGAA 112 347 157 Antisense
GAATGACTGAATCTGACATACAATC 236 331 179 Antisense
GAGGCAGAGGATTTTGAGTTCGGAG 573 405 340 Antisense
ATGAACTTCATGAATCCTTCTGGAA 84 45 404 Antisense
GAAACTACGTCCAGATCAGCATACA 488 357 426 Antisense
CCAGATCAGCATACAGTCTTGCATT 531 271 436 Antisense
GCAAGCAGCATTGTCGCTGAGGCTT 307 321 559 Antisense
GCTGAGGCTTCATCATTGGCTAATC 36 297 574 Antisense
TTGGCTAATCAAATTCTGTTAAGCA 29 709 589 Antisense
GGATATGAGACTGTTGCTTCAACTC 574 509 619 Antisense
GCTTCAACTCTCTTGAGTTCTCGTA 571 305 634 Antisense
Sequence Source Affymetrix Proprietary Database