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[177111_at]

Description Y73B6BL.J /REP_DB=WormBase Gene ID /WP=CE26283 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23469.1
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 45      4grp_high_8_8 > 08      6grp_highest_nonorm > 83      6grp_total > 76      4grp_total > 23      6grp_highest_sub > Da      6grp_cgc > 47      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 446
1 2 3 4 range
A 617
(3)
P 		300.6
(-2.7)
P 		284.2
(-3)
P 		536.2
(1.5)
P 		333
B 636
(3)
P 		374.9
(-3)
P 		410.9
(-2.2)
P 		609
(2.4)
P 		261
C 703.2
(3)
P 		257.1
(-3)
P 		425.9
(-0.7)
P 		556.5
(1)
P 		446
avr 652.1
(3) 		310.9
(-3) 		373.7
(-1.9) 		567.2
(1.5) 		341

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 342
1 2 3 4 5 6 range
A 369.1
(-1.9)
P 		552.8
(1.3)
P 		305.3
(-3)
P 		570.9
(1.7)
P 		423.4
(-0.9)
P 		647.4
(3)
P 		342
B 432.5
(-0.4)
P 		334.1
(-3)
P 		459.7
(0.3)
P 		400
(-1.2)
P 		399.3
(-1.3)
P 		559.2
(3)
P 		225
C 522.2
(1)
P 		368.8
(-3)
P 		566.8
(2.2)
P 		502.6
(0.5)
P 		450.7
(-0.9)
P 		597.4
(3)
P 		229
avr 441.3
(-2.3) 		418.6
(-3) 		443.9
(-2.2) 		491.2
(-0.6) 		424.5
(-2.8) 		601.3
(3) 		183
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 224.7
(3)
P 		65.2
(-3)
P 		74.3
(-2.7)
P 		78.5
(-2.5)
P 		160
B 202.8
(3)
P 		92.6
(-3)
P 		95.2
(-2.9)
P 		129.8
(-1)
P 		110
C 232.8
(3)
P 		73.9
(-3)
P 		148.4
(-0.2)
P 		93.8
(-2.2)
P 		159
avr 220.1
(3) 		77.2
(-3) 		106
(-1.8) 		100.7
(-2) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 68
(-2.9)
P 		196.6
(3)
P 		66.5
(-3)
P 		144.8
(0.6)
P 		127.8
(-0.2)
P 		157.6
(1.2)
P 		130
B 150.5
(-0.4)
P 		111.2
(-2.7)
P 		111.6
(-2.7)
P 		105.9
(-3)
P 		162.2
(0.3)
P 		208.5
(3)
P 		103
C 193.6
(2.6)
P 		112.5
(-2.3)
P 		144.3
(-0.4)
P 		101.1
(-3)
P 		145.8
(-0.3)
P 		200
(3)
P 		99
avr 137.4
(-0.8) 		140.1
(-0.6) 		107.5
(-3) 		117.3
(-2.3) 		145.3
(-0.2) 		188.7
(3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177111_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19164
Sequence Type Exemplar sequence
Representative Public ID Y73B6BL.J NCBI
Target Description Y73B6BL.J /REP_DB=WormBase Gene ID /WP=CE26283 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23469.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068573(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068573 NCBI Caenorhabditis elegans Suppressor with Morphological effect on Genitalia family member (smg-3) (smg-3) mRNA, complete cds. 11/11 A 
SNAP00000032049 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6367920:6373071:-1 11/11 None
GENEFINDER00000032091 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6364863:6373071:-1 11/11 None
Y73B6BL.18.1 ENSEMBL cdna:known chromosome:CEL140:IV:6362420:6373071:-1 gene:Y73B6BL.18 11/11 A 
Y73B6BL.18.2 ENSEMBL cdna:known chromosome:CEL140:IV:6362472:6373071:-1 gene:Y73B6BL.18 11/11 A 
Y73B6BL.18.3 ENSEMBL cdna:known chromosome:CEL140:IV:6362477:6373071:-1 gene:Y73B6BL.18 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6368042-6383972(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol smg-3
Ensembl Y73B6BL.18
Entrez Gene 177397 Entrez gene
SwissProt Q95XD2 EMBL-EBI
WormBase CE29923 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68590 Suppressor with morphological effect on genitalia protein 3 [Caenorhabditis elegans] ref|NP_500974.2| Suppressor with Morphological effect on Genitalia family member (smg-3) [Caenorhabditis elegans] 0.0
blast CAE61554 Hypothetical protein CBG05463 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003890 EMBL-EBI Initiation factor eIF-4 gamma, middle 4.4E-5
Pfam IPR003890 EMBL-EBI Initiation factor eIF-4 gamma, middle 4.3E-9
Pfam IPR003890 EMBL-EBI Initiation factor eIF-4 gamma, middle 8.2E-5
Sequence
Target Sequence 
>C. ELEGANS:177111_AT
aaaatcctctcgatgttcagacaagtttcccgtatgaagtcgaacttgcgtatactgaat
tgtgccgtcaattccgccaaaagaagtccaattcgcttcgatggccgaaaaatttgaaag
aagctcaagatatagtcacaaaaattgagaagaaattcaagggagaggtagacaaactat
tgaaagagtttgccggagaacacaaagacattgaaggtgatggtttgaatgaactgaatg
ttattgaagaagatgatgaagaagacgaagatgagaatcgagactctgaactggatgaag
atgattctgaagaagatcgacagaaaattgagaatttctcgaatgccgtgacaaaagaag
aagatgacgagttccagagagaactcgatcgaatgatgggagaaggattcagacaatcag
cgatgcctgtagctgctgctcaatacgatgtcactctaccagctgctgtaaaagatcggt
tca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAATCCTCTCGATGTTCAGACAAG 506 131 6047 Antisense
GATGTTCAGACAAGTTTCCCGTATG 498 409 6058 Antisense
GTTTCCCGTATGAAGTCGAACTTGC 581 449 6071 Antisense
CTTGCGTATACTGAATTGTGCCGTC 407 217 6091 Antisense
GCCGTCAATTCCGCCAAAAGAAGTC 46 283 6110 Antisense
AAAGAAGTCCAATTCGCTTCGATGG 579 123 6126 Antisense
GAATTTCTCGAATGCCGTGACAAAA 521 325 6378 Antisense
ATCAGCGATGCCTGTAGCTGCTGCT 641 27 6462 Antisense
AGCTGCTGCTCAATACGATGTCACT 158 81 6477 Antisense
TCAATACGATGTCACTCTACCAGCT 198 633 6486 Antisense
CCAGCTGCTGTAAAAGATCGGTTCA 423 267 6505 Antisense
Sequence Source Affymetrix Proprietary Database