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[177105_s_at]

Description F54B11.3 /REP_DB=WormBase Gene ID /WP=CE05927 /CHR=X /FEA=Sanger Annotation /DEF=locus:unc-84 (HINXTON) TR:Q20745
Cluster 6grp_highest_10_10 > 53      6grp_high1_10_10 > 35      6grp_highest_nonorm > 60      6grp_total > 35      4grp_total > 52      6grp_highest_sub > 02      6grp_cgc > 43      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 447
1 2 3 4 range
A 391.4
(2.3)
P 		337.7
(1.2)
P 		426.8
(3)
P 		124.2
(-3)
A 		303
B 281.1
(0.8)
P 		404.7
(3)
P 		320.7
(1.5)
P 		69
(-3)
A 		336
C 409.7
(1.5)
P 		515.8
(3)
P 		367
(0.9)
P 		85.5
(-3)
A 		430
avr 360.7
(1.9) 		419.4
(3) 		371.5
(2.1) 		92.9
(-3) 		327

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 313
1 2 3 4 5 6 range
A 402.6
(0.5)
P 		529.2
(3)
P 		298.8
(-1.6)
P 		283.5
(-1.9)
P 		228.8
(-3)
P 		327.3
(-1)
P 		300
B 334.5
(-0.4)
P 		488.5
(3)
P 		311.5
(-0.9)
P 		300.3
(-1.2)
P 		216.7
(-3)
P 		286.1
(-1.5)
P 		272
C 482.9
(2.6)
P 		503.2
(3)
P 		323.5
(-0.9)
P 		226.3
(-3)
P 		312.2
(-1.1)
P 		335.3
(-0.6)
P 		277
avr 406.7
(0.6) 		507
(3) 		311.3
(-1.6) 		270
(-2.6) 		252.6
(-3) 		316.2
(-1.5) 		254
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 134
1 2 3 4 range
A 142.5
(3)
P 		73.3
(-0.3)
P 		111.6
(1.5)
P 		18.2
(-3)
A 		124
B 89.6
(2.3)
P 		100
(3)
P 		74.3
(1.2)
P 		14.7
(-3)
A 		85
C 135.6
(2.4)
P 		148.3
(3)
P 		127.9
(2.1)
P 		14.4
(-3)
A 		134
avr 122.6
(3) 		107.2
(2.1) 		104.6
(2) 		15.8
(-3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 74.2
(-2.6)
P 		188.2
(3)
P 		65.1
(-3)
P 		71.9
(-2.7)
P 		69
(-2.8)
P 		79.7
(-2.3)
P 		123
B 116.4
(-0.2)
P 		162.6
(3)
P 		75.6
(-3)
P 		79.5
(-2.7)
P 		88
(-2.1)
P 		106.7
(-0.9)
P 		87
C 179
(3)
P 		153.5
(1.9)
P 		82.3
(-1.3)
P 		45.5
(-3)
P 		101
(-0.5)
P 		112.2
(-0)
P 		134
avr 123.2
(0.4) 		168.1
(3) 		74.3
(-2.5) 		65.6
(-3) 		86
(-1.8) 		99.5
(-1) 		103
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177105_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17373
Sequence Type Exemplar sequence
Representative Public ID F54B11.3 NCBI
Target Description F54B11.3 /REP_DB=WormBase Gene ID /WP=CE05927 /CHR=X /FEA=Sanger Annotation /DEF=locus:unc-84 (HINXTON) TR:Q20745
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029536(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029536 NCBI Caenorhabditis elegans UNCoordinated family member (unc-84) (unc-84) mRNA, complete cds. 11/11 None
SNAP00000008286 ENSEMBL cdna:SNAP chromosome:CEL140:X:13584699:13589415:1 11/11 None
GENEFINDER00000008301 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13584699:13589415:1 11/11 None
F54B11.3a ENSEMBL cdna:known chromosome:CEL140:X:13584694:13589742:1 gene:F54B11.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181480 2 1 NM_001029536 175676_at (11), 177105_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13584698-13589415(+) 100.0 100.0
Public Domain and Genome References
Ensembl F54B11.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94142 Hypothetical protein F54B11.3a [Caenorhabditis elegans] ref|NP_001024707.1| UNCoordinated family member (unc-84) [Caenorhabditis elegans] gb|AAF15883.1| UNC-84A [Caenorhabditis elegans] sp|Q20745|UNC84_CAEEL Nuclear migration and anchoring protein unc-84 (Uncoordinated protein 84) 0.0
blast T22608 hypothetical protein F54B11.3 - Caenorhabditis elegans 0.0
blast CAC42306 Hypothetical protein F54B11.3b [Caenorhabditis elegans] ref|NP_001024708.1| UNCoordinated family member (unc-84) [Caenorhabditis elegans] gb|AAF15884.1| UNC-84B [Caenorhabditis elegans] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024707.1 7 117-134,138-157,390-412,508-530,678-700,715-737,757-779
F54B11.3a 7 117-134,138-157,390-412,508-530,678-700,715-737,757-779
SNAP00000008286 5 117-134,138-157,390-412,478-500,513-532
GENEFINDER00000008301 7 117-134,138-157,390-412,508-530,690-712,727-749,769-791
Sequence
Target Sequence 
>C. ELEGANS:177105_S_AT
aagttgactatgccctggagagctcaggtggagctgttgtgtcaacaagatgctcggaga
cgtacaaaagctacacaaggctggaaaagttttgggatatcccaatctactatttccatt
actctccaagagttgtcattcagagaaattccaaatccctgtttcctggggaatgctggt
gcttcaaagaatcccgtggctacattgctgtcgagctgtctcatttcattgatgtttcta
gcatcagctatgagcacattggatcagaagttgctccagaagggaaccggtcgagtgctc
caaagggagtcctcgtttgggcttacaagcagattgacgacctgaactcgagagttttga
ttggcgactacacttatgatcttgatggcccgccacttcaattcttccttgccaagcaca
aacccgattttcctgtcaagtttgtggagctcgaggtgacaagcaattacggagctccgt
tcacatgtctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTTGACTATGCCCTGGAGAGCTC 219 155 2855 Antisense
TGGGATATCCCAATCTACTATTTCC 4 555 2947 Antisense
TACTATTTCCATTACTCTCCAAGAG 695 645 2962 Antisense
AGAAATTCCAAATCCCTGTTTCCTG 240 71 2998 Antisense
TTTCCTGGGGAATGCTGGTGCTTCA 142 667 3016 Antisense
CAAAGAATCCCGTGGCTACATTGCT 249 189 3039 Antisense
GCTGTCGAGCTGTCTCATTTCATTG 460 295 3061 Antisense
GTCCTCGTTTGGGCTTACAAGCAGA 526 475 3163 Antisense
TTCTTCCTTGCCAAGCACAAACCCG 409 687 3256 Antisense
CACAAACCCGATTTTCCTGTCAAGT 155 195 3271 Antisense
TTACGGAGCTCCGTTCACATGTCTC 607 681 3321 Antisense
Sequence Source Affymetrix Proprietary Database