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[177103_at]

Description Y61A9LA.M /REP_DB=WormBase Gene ID /WP=CE22688 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60845.1
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 49      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 82      6grp_total > 94      4grp_total > 24      6grp_high1_sub > Cu      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 204.8
(-3)
P 		212.8
(-2.5)
P 		302.2
(3)
P 		296
(2.6)
P 		97
B 221.6
(-2.7)
P 		217.1
(-3)
P 		239.8
(-1.7)
P 		321.5
(3)
P 		104
C 216
(-3)
P 		246.1
(0.8)
P 		253.5
(1.7)
P 		264.1
(3)
P 		48
avr 214.1
(-3) 		225.3
(-2.2) 		265.2
(0.8) 		293.9
(3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 315
1 2 3 4 5 6 range
A 480.5
(3)
P 		317.1
(-0.4)
P 		189.4
(-3)
P 		419.4
(1.7)
P 		446.4
(2.3)
P 		478
(2.9)
P 		291
B 318.2
(-1.6)
P 		306.2
(-2)
P 		275.3
(-3)
P 		453
(3)
P 		436.9
(2.5)
P 		345.2
(-0.6)
P 		178
C 459.4
(1.9)
P 		336.4
(-1.2)
P 		262.9
(-3)
P 		388.5
(0.1)
P 		504.6
(3)
P 		324.8
(-1.5)
P 		242
avr 419.4
(1.8) 		319.9
(-0.9) 		242.5
(-3) 		420.3
(1.8) 		462.6
(3) 		382.7
(0.8) 		220
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 74.6
(2.3)
P 		46.2
(-2.5)
P 		79
(3)
P 		43.3
(-3)
P 		36
B 70.7
(3)
P 		53.6
(-3)
P 		55.6
(-2.3)
P 		68.5
(2.2)
P 		17
C 71.5
(0.7)
P 		70.8
(0.6)
P 		88.4
(3)
P 		44.5
(-3)
P 		44
avr 72.3
(2.5) 		56.9
(-1.7) 		74.3
(3) 		52.1
(-3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 136
1 2 3 4 5 6 range
A 88.6
(0)
P 		112.8
(1.6)
P 		41.3
(-3)
P 		106.4
(1.2)
P 		134.7
(3)
P 		116.4
(1.8)
P 		93
B 110.7
(-0.6)
P 		101.9
(-1.1)
P 		66.9
(-3)
P 		120
(-0.1)
P 		177.5
(3)
P 		128.7
(0.4)
P 		111
C 170.3
(3)
P 		102.6
(-0.9)
P 		66.9
(-3)
P 		78.1
(-2.4)
P 		163.3
(2.6)
P 		108.7
(-0.6)
P 		103
avr 123.2
(0.9) 		105.8
(-0.2) 		58.4
(-3) 		101.5
(-0.4) 		158.5
(3) 		117.9
(0.6) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177103_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19496
Sequence Type Exemplar sequence
Representative Public ID Y61A9LA.M NCBI
Target Description Y61A9LA.M /REP_DB=WormBase Gene ID /WP=CE22688 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60845.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071842(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071842 NCBI Caenorhabditis elegans Y61A9LA.1 (Y61A9LA.1) mRNA, complete cds. 11/11 None
SNAP00000010103 ENSEMBL cdna:SNAP chromosome:CEL140:V:4598009:4601416:1 11/11 None
GENEFINDER00000010117 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4598009:4601416:1 11/11 None
Y61A9LA.1 ENSEMBL cdna:known chromosome:CEL140:V:4592652:4601828:1 gene:Y61A9LA.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4598008-4601416(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y61A9LA.1
Entrez Gene 178849 Entrez gene
SwissProt Q9N309 EMBL-EBI
WormBase CE29895 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4182 carboxypeptidase A activity inferred from electronic annotation QuickGO AmiGO
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60845 Hypothetical protein Y61A9LA.1 [Caenorhabditis elegans] ref|NP_504243.2| Y61A9LA.1 [Caenorhabditis elegans] 0.0
blast CAE64152 Hypothetical protein CBG08770 [Caenorhabditis briggsae] 0.0
blast CAG11830 unnamed protein product [Tetraodon nigroviridis] 2.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 1.2E-28
Pfam IPR005828 EMBL-EBI General substrate transporter 1.2E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504243.2 12 35-57,90-112,124-146,151-173,186-208,212-234,314-336,356-378,385-407,417-439,446-465,475-497
Y61A9LA.1 12 35-57,90-112,124-146,151-173,186-208,212-234,314-336,356-378,385-407,417-439,446-465,475-497
SNAP00000010103 11 58-80,92-114,119-141,154-176,180-202,282-304,324-346,353-375,385-407,414-433,443-465
GENEFINDER00000010117 11 58-80,92-114,119-141,154-176,180-202,282-304,324-346,353-375,385-407,414-433,443-465
Sequence
Target Sequence 
>C. ELEGANS:177103_AT
aaattgcgtacgtcttcactggcgcccgatcccttcgcggctacagcacattcattctct
acactctgtcacacttcacctactctcaagcgacgtggctctcgtttgccaccggtctcc
tgcgtctcccgttcaccctggttccagtcttcctggtcgatcgactcggtcgtcgtccac
ttattattgtttcgatgctcgtctcctttgtgtccatcttggtgatgatcattgccatca
atattaatggagagttgaagtatgccacattcatcggccttaccgtactccttctcgtga
acacttgtggaatcggatcagtttcaaggttctacgcagctgagcttgttccaaggaatc
tcctattgtcgtcagtttccacgttgacaatgttcgaagcgttgacgaagattggagtgg
aattcgcattttatccaaccgccaatgtgatcggcgctcaatcgcttctcctattcctca
ttccaaccggagttttcaccgtaatgtgctacctgatgtgcccggaaacgagccgaatga
ctgtcaacgaggtgctcaataatgtggccgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATTGCGTACGTCTTCACTGGCGC 606 115 893 Antisense
GCTACAGCACATTCATTCTCTACAC 22 303 932 Antisense
GTCGTCGTCCACTTATTATTGTTTC 340 473 1061 Antisense
TCTCCTTTGTGTCCATCTTGGTGAT 278 611 1094 Antisense
AGTATGCCACATTCATCGGCCTTAC 334 63 1151 Antisense
GGTTCTACGCAGCTGAGCTTGTTCC 110 507 1220 Antisense
GAATCTCCTATTGTCGTCAGTTTCC 459 327 1248 Antisense
GGAATTCGCATTTTATCCAACCGCC 641 533 1311 Antisense
AATGTGATCGGCGCTCAATCGCTTC 707 163 1336 Antisense
CGTAATGTGCTACCTGATGTGCCCG 216 247 1392 Antisense
GAGGTGCTCAATAATGTGGCCGCCA 143 403 1441 Antisense
Sequence Source Affymetrix Proprietary Database