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[177074_at]

Description Y46C8AL.I /REP_DB=WormBase Gene ID /WP=CE25296 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60603.1
Cluster 6grp_total > 37      4grp_total > 32      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 72.3
(3)
A 		10.8
(-3)
A 		35.8
(-0.6)
A 		42
(0)
A 		62
B 20.7
(-3)
A 		27.2
(-1.6)
A 		47.9
(3)
A 		35.9
(0.4)
A 		27
C 9.2
(-3)
A 		81.4
(3)
A 		45.5
(0)
A 		55.9
(0.9)
A 		72
avr 34.1
(-3) 		39.8
(0.3) 		43.1
(2.1) 		44.6
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 80.6
(3)
A 		23.7
(-1.4)
A 		5.4
(-2.8)
A 		16.8
(-1.9)
A 		2.5
(-3)
A 		64.3
(1.7)
P 		78
B 10
(-2.7)
A 		31.8
(3)
A 		8.9
(-3)
A 		9
(-3)
A 		19.5
(-0.2)
A 		29.8
(2.5)
A 		23
C 14.4
(-1.3)
A 		34.7
(2.3)
A 		36.7
(2.7)
A 		38.3
(3)
A 		28.4
(1.2)
A 		5.1
(-3)
A 		33
avr 35
(3) 		30.1
(1.4) 		17
(-2.9) 		21.4
(-1.5) 		16.8
(-3) 		33.1
(2.4) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 26.3
(3)
A 		2.3
(-3)
A 		9.4
(-1.2)
A 		6.1
(-2.1)
A 		24
B 6.6
(-3)
A 		6.7
(-2.9)
A 		11.1
(3)
A 		7.7
(-1.5)
A 		5
C 3.1
(-3)
A 		23.4
(3)
A 		15.8
(0.8)
A 		9.4
(-1.1)
A 		20
avr 12
(2.9) 		10.8
(1.2) 		12.1
(3) 		7.7
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 14.9
(2.7)
A 		8.4
(0.1)
A 		1.2
(-2.8)
A 		4.3
(-1.6)
A 		0.8
(-3)
A 		15.7
(3)
P 		15
B 3.5
(-2.1)
A 		10.6
(2.7)
A 		2.2
(-3)
A 		2.4
(-2.9)
A 		7.9
(0.8)
A 		11.1
(3)
A 		9
C 5.3
(-0.6)
A 		10.6
(3)
A 		9.3
(2.1)
A 		7.7
(1)
A 		9.2
(2.1)
A 		1.7
(-3)
A 		9
avr 7.9
(0.9) 		9.9
(3) 		4.2
(-3) 		4.8
(-2.4) 		6
(-1.1) 		9.5
(2.6) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177074_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19186
Sequence Type Exemplar sequence
Representative Public ID Y46C8AL.I NCBI
Target Description Y46C8AL.I /REP_DB=WormBase Gene ID /WP=CE25296 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60603.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068042(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068042 NCBI Caenorhabditis elegans Y46C8AL.6 (Y46C8AL.6) mRNA, complete cds. 11/11 None
SNAP00000030671 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3951732:3953242:1 10/11 None
GENEFINDER00000030685 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3948423:3953242:1 10/11 None
Y46C8AL.6 ENSEMBL cdna:known chromosome:CEL140:IV:3948423:3952256:1 gene:Y46C8AL.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3948428-3952262(+) 93.75 93.75
Public Domain and Genome References
Ensembl Y46C8AL.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60603 Hypothetical protein Y46C8AL.6 [Caenorhabditis elegans] ref|NP_500443.2| Y46C8AL.6 [Caenorhabditis elegans] 0.0
blast NP_504664 K04A8.7 [Caenorhabditis elegans] 4.0E-92
blast NP_504664 K04A8.7 [Caenorhabditis elegans] 5.0E-92
blast T25782 hypothetical protein F47D2.2 - Caenorhabditis elegans 1.0E-91
blast NP_492844 F49D11.5 [Caenorhabditis elegans] 1.0E-73
blast AAF60603 Hypothetical protein Y46C8AL.6 [Caenorhabditis elegans] ref|NP_500443.2| Y46C8AL.6 [Caenorhabditis elegans] 3.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 2.5E-33
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 2.5E-33
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000030685 3 81-103,113-135,447-469
Sequence
Target Sequence 
>C. ELEGANS:177074_AT
ggaaagatatcccatacacgcttccaacccgagggtatctggtacagggtcccaaacact
tccgtcgatgtctgttcctttcgaaaatgaatatttattaatggtggcacttcaaaatca
cgtgccagtaagcaatgttcaatctgtgacggtttcatggtataatgatgcttcctatcg
ctctggaaattttctaatgcaacctcctggttggacatatatcagtagtaatgatgacgg
tacccgtgagcatcttgaagagtacatctataatgtaactatagattatgactacaccaa
tagtaatgtcgaaactatgcaagtcagattctacagtccattccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAAGATATCCCATACACGCTTCC 541 527 1701 Antisense
ACGCTTCCAACCCGAGGGTATCTGG 205 35 1718 Antisense
GGTATCTGGTACAGGGTCCCAAACA 532 493 1734 Antisense
CAAACACTTCCGTCGATGTCTGTTC 526 191 1753 Antisense
GATGTCTGTTCCTTTCGAAAATGAA 297 407 1767 Antisense
AATGGTGGCACTTCAAAATCACGTG 83 163 1800 Antisense
GTTCAATCTGTGACGGTTTCATGGT 367 445 1837 Antisense
TGATGCTTCCTATCGCTCTGGAAAT 98 567 1866 Antisense
AATGCAACCTCCTGGTTGGACATAT 532 165 1896 Antisense
GATGACGGTACCCGTGAGCATCTTG 567 411 1933 Antisense
AAGTCAGATTCTACAGTCCATTCCC 678 155 2021 Antisense
Sequence Source Affymetrix Proprietary Database