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[177068_at]

Description Y94H6A.E /REP_DB=WormBase Gene ID /WP=CE25632 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60891.1
Cluster 6grp_total > 62      4grp_total > 34      6grp_cgc > 73      6grp_cgc_sub > 02_03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 67.5
(-3)
A 		132.9
(1.3)
A 		82.7
(-2)
A 		159.5
(3)
A 		92
B 100.5
(0.3)
A 		112.2
(1.4)
A 		129
(3)
A 		64.6
(-3)
A 		64
C 52.6
(-3)
A 		131.7
(3)
A 		92.9
(0.1)
A 		109.7
(1.3)
A 		79
avr 73.5
(-3) 		125.6
(3) 		101.5
(0.2) 		111.3
(1.4) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 105.8
(0.3)
A 		101.9
(-1.6)
A 		99.7
(-2.7)
A 		111.2
(3)
A 		100.9
(-2.1)
A 		99
(-3)
A 		12
B 80.6
(-0.1)
A 		107.8
(2.8)
A 		109.9
(3)
A 		105.8
(2.6)
A 		99.4
(1.9)
A 		53
(-3)
A 		57
C 58.9
(-3)
A 		96.2
(-0.1)
A 		136.1
(3)
A 		87.9
(-0.7)
A 		82.6
(-1.2)
A 		86.1
(-0.9)
A 		77
avr 81.8
(-2.6) 		102
(0.8) 		115.2
(3) 		101.6
(0.7) 		94.3
(-0.5) 		79.4
(-3) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 24.6
(-0.5)
A 		28.9
(3)
A 		21.6
(-3)
A 		23.4
(-1.5)
A 		7
B 32
(3)
A 		27.7
(1.6)
A 		29.9
(2.3)
A 		13.8
(-3)
A 		18
C 17.4
(-3)
A 		37.9
(3)
A 		32.4
(1.4)
A 		18.5
(-2.7)
A 		21
avr 24.7
(-0.2) 		31.5
(3) 		28
(1.4) 		18.6
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 19.5
(-3)
A 		36.2
(3)
A 		21.7
(-2.2)
A 		28.2
(0.1)
A 		30.4
(0.9)
A 		24.1
(-1.3)
A 		17
B 28
(-0.6)
A 		35.9
(1.7)
A 		26.7
(-1)
A 		28
(-0.6)
A 		40.4
(3)
A 		19.8
(-3)
A 		21
C 21.8
(-1.5)
A 		29.4
(1.2)
A 		34.6
(3)
A 		17.7
(-3)
A 		26.7
(0.2)
A 		28.8
(0.9)
A 		17
avr 23.1
(-3) 		33.8
(3) 		27.7
(-0.4) 		24.6
(-2.2) 		32.5
(2.3) 		24.2
(-2.4) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177068_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19299
Sequence Type Exemplar sequence
Representative Public ID Y94H6A.E NCBI
Target Description Y94H6A.E /REP_DB=WormBase Gene ID /WP=CE25632 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60891.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067844(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067844 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-8) (ubc-8) mRNA, complete cds. 9/11 None
GENEFINDER00000003293 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2704913:2707228:-1 11/11 None
Y94H6A.6 ENSEMBL cdna:known chromosome:CEL140:IV:2704690:2707228:-1 gene:Y94H6A.6 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000003280 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2704695-2707234(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ubc-8
Ensembl Y94H6A.6
Entrez Gene 190802 Entrez gene
SwissProt Q9N2W9 EMBL-EBI
WormBase CE34621 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17427_AT ubiquitin-conjugating enzyme 4 (UBC4) at
ATH1-121501:249299_AT ubiquitin-conjugating enzyme 4 (UBC4) at
CANINE_2:CFA.2460.1.A1_AT similar to ubiquitin-conjugating enzyme E2H isoform 2 cfa
CANINE_2:CFAAFFX.3148.1.S1_AT similar to ubiquitin-conjugating enzyme E2H isoform 2 cfa
CANINE_2:CFAAFFX.3149.1.S1_S_AT similar to ubiquitin-conjugating enzyme E2H isoform 2 cfa
DROSOPHILA_2:1629843_S_AT dm
DROSGENOME1:153913_AT dm
CHICKEN:GGAAFFX.11850.1.S1_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) gga
CHICKEN:GGA.1365.1.S1_AT Similar to Ubiquitin-conjugating enzyme E2 H (Ubiquitin-protein ligase H) (Ubiquitin carrier protein H) (UBCH2) (E2-20K) gga
HG-U95AV2:369_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HC-G110:369_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133A:217799_X_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:217799_X_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133A_2:217799_X_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:222421_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:222421_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:222419_X_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:222419_X_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:222420_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:222420_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HU35KSUBA:AA095473_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HU35KSUBD:RC_N90224_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HUGENEFL:Z29331_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:G4507782_3P_A_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.28505.2.S1_3P_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.28505.0.S4_3P_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.28505.0.S3_3P_A_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.28505.0.S1_3P_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133A_2:221962_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-FOCUS:221962_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:221962_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133A:221962_S_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U95E:91147_AT ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:229483_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:229483_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HU35KSUBD:RC_AA485121_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.101340.0.A1_3P_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U95B:49883_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:226637_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:226637_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:226681_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:226681_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:227950_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:227950_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:237232_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:237232_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133_PLUS_2:239721_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U133B:239721_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U95B:58777_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U95D:72445_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U95E:81364_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HG-U95D:82691_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HU35KSUBA:AA043601_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HU35KSUBC:RC_R11047_S_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
HU35KSUBD:RC_AA215685_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.111219.0.A1_3P_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.119275.0.A1_3P_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.28505.1.A1_3P_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.28505.1.A2_3P_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
U133_X3P:HS.28505.2.A1_3P_AT Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) hs
MG-U74AV2:100528_AT ubiquitin-conjugating enzyme E2H mm
MU11KSUBA:AA396357_S_AT ubiquitin-conjugating enzyme E2H mm
MU11KSUBA:U19854_S_AT ubiquitin-conjugating enzyme E2H mm
MG-U74AV2:100529_AT ubiquitin-conjugating enzyme E2H mm
MOE430A:1418631_AT ubiquitin-conjugating enzyme E2H mm
MOUSE430A_2:1418631_AT ubiquitin-conjugating enzyme E2H mm
MOUSE430_2:1418631_AT ubiquitin-conjugating enzyme E2H mm
MOE430A:1418632_AT ubiquitin-conjugating enzyme E2H mm
MOUSE430A_2:1418632_AT ubiquitin-conjugating enzyme E2H mm
MOUSE430_2:1418632_AT ubiquitin-conjugating enzyme E2H mm
MOE430B:1428791_AT ubiquitin-conjugating enzyme E2H mm
MOUSE430_2:1428791_AT ubiquitin-conjugating enzyme E2H mm
MOE430B:1438971_X_AT ubiquitin-conjugating enzyme E2H mm
MOUSE430_2:1438971_X_AT ubiquitin-conjugating enzyme E2H mm
MOUSE430_2:1447374_AT Ubiquitin-conjugating enzyme E2H, mRNA (cDNA clone MGC:6029 IMAGE:3600233) mm
MOE430B:1447374_AT Ubiquitin-conjugating enzyme E2H, mRNA (cDNA clone MGC:6029 IMAGE:3600233) mm
MU19KSUBA:TC24065_AT Ubiquitin-conjugating enzyme E2H, mRNA (cDNA clone MGC:6029 IMAGE:3600233) mm
YEAST_2:1780036_AT Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60891 Ubiquitin conjugating enzyme protein 8 [Caenorhabditis elegans] ref|NP_500245.2| UBiquitin Conjugating enzyme family member (ubc-8) [Caenorhabditis elegans] 1.0E-109
blast AAF60891 Ubiquitin conjugating enzyme protein 8 [Caenorhabditis elegans] ref|NP_500245.2| UBiquitin Conjugating enzyme family member (ubc-8) [Caenorhabditis elegans] 2.0E-82
blast NP_958897 ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) [Danio rerio] gb|AAH49139.1| Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) [Danio rerio] emb|CAI20751.1| ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) [Danio rerio] emb|CAH68834.1| ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) [Danio rerio] ref|XP_702512.1| PREDICTED: hypothetical protein XP_697420 [Danio rerio] 1.0E-61
blast AAH44038 Ube2h-prov protein [Xenopus laevis] ref|NP_001004909.1| MGC89025 protein [Xenopus tropicalis] gb|AAH75340.1| MGC89025 protein [Xenopus tropicalis] 1.0E-61
blast CAE68237 Hypothetical protein CBG13911 [Caenorhabditis briggsae] 1.0E-47
blast NP_958897 ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) [Danio rerio] gb|AAH49139.1| Ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) [Danio rerio] emb|CAI20751.1| ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) [Danio rerio] emb|CAH68834.1| ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) [Danio rerio] ref|XP_702512.1| PREDICTED: hypothetical protein XP_697420 [Danio rerio] 1.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 9.3E-49
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 1.1E-39
Sequence
Target Sequence 
>C. ELEGANS:177068_AT
aaaattcttgcagctgcctacgagaatggtgtgtggagaattcgtgtcgatatgccggac
aaatacccgttcaaatcgccgtccatcgggtttttgaacaagattttccacccgaatatc
gatgaagcttcgggcaccgtatgccttgatgtcataaatcaggtaggaattggtggccga
agtgtgtggaaagcctggacagccctctacgatctcacaaacattttcgataccttcctg
cctcaacttctcacctacccgaatgcagcagaccccttgaacggagaagccgcccggtta
tacattcacaagccagaggaatacagaagaacgtgtagagaatacgtaatgcgatttgca
agtgaacaccgtgccatagaaggatgctccccgggaaacgcctattccatatcctcaaca
ccctcggagcactacgatggtcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAATTCTTGCAGCTGCCTACGAGA 625 133 202 Antisense
GACAAATACCCGTTCAAATCGCCGT 120 367 259 Antisense
TCAAATCGCCGTCCATCGGGTTTTT 183 629 272 Antisense
AAGATTTTCCACCCGAATATCGATG 372 153 301 Antisense
ATGAAGCTTCGGGCACCGTATGCCT 602 43 323 Antisense
TGTGTGGAAAGCCTGGACAGCCCTC 599 555 384 Antisense
ACAAACATTTTCGATACCTTCCTGC 23 113 418 Antisense
AGCAGACCCCTTGAACGGAGAAGCC 8 73 468 Antisense
AGAAGCCGCCCGGTTATACATTCAC 170 71 486 Antisense
CCGGGAAACGCCTATTCCATATCCT 265 265 592 Antisense
ACCCTCGGAGCACTACGATGGTCAC 479 85 621 Antisense
Sequence Source Affymetrix Proprietary Database